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CCD Camera for Cryo-EM

用于冷冻电镜的 CCD 相机

基本信息

批准号：
7585893
负责人：
Huilin Li
金额：
$ 24.4万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2010-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We request a 4K by 4K CCD camera designed for cryo-EM. The camera will be installed in a JEOL JEM2010 FasTEM/STEM microscope. The cryo-EM facility is dedicated to studying the 3D structures of biological macromolecules. These protein complexes play critical roles in human health and disease. The microscope is located in Brookhaven National Lab and operated by Huilin Li, who holds a joint appointment between BNL and Biochemistry and Cell Biology Department in Stony Brook University. The large format camera and its associated software will enable microscopy automation and cryo-TEM and cryo-STEM bimodal tomography, thus significantly increase the microscopy throughput and research productivity. There are three large institutes on Long Island, New York, that conduct NIH-supported research. These are the so-called Long Island Tri-Institutes: SUNY Stony Brook being in the middle, Brookhaven National Laboratory about 15 miles to the east, and Cold Spring Harbor Laboratory about 15 miles to the west. There are numerous needs for cryo-EM imaging of large biological assemblies from local researchers. However, our cryo-EM group is the only facility available on Long Island. The requested instrument will facilitate existing NIH-funded cryo-EM projects, with the likelihood of accommodating several more projects from local biomedical community. Public Health Relevance: We request a CCD camera designed for recording digital images in an electron microscope. The microscopy facility is dedicated to studying the 3D structures of biological macromolecules. These protein complexes play critical roles in human health and disease. The large format camera and its associated software will enable microscopy automation and significantly increase the microscopy throughput and research productivity.

描述（由申请人提供）：我们要求为冷冻EM设计的4K × 4K CCD相机。摄像机将安装在JEOL JEM 2010 FastTEM/STEM显微镜中。cryo-EM设施致力于研究生物大分子的3D结构。这些蛋白质复合物在人类健康和疾病中起着关键作用。该显微镜位于布鲁克海文国家实验室，由Huilin Li操作，他持有BNL和斯托尼布鲁克大学生物化学和细胞生物学系的联合任命。大画幅相机及其相关软件将实现显微镜自动化和cryo-TEM和cryo-STEM双峰断层扫描，从而显着提高显微镜的吞吐量和研究生产力。在纽约的长岛有三个大型研究所，进行NIH支持的研究。这就是所谓的长岛三大研究所：纽约州立大学斯托尼布鲁克在中间，布鲁克海文国家实验室在东边约15英里，冷泉港实验室在西边约15英里。当地研究人员对大型生物组件的冷冻EM成像有许多需求。然而，我们的冷冻EM组是长岛唯一可用的设施。所要求的仪器将促进现有的NIH资助的冷冻EM项目，并有可能容纳来自当地生物医学界的多个项目。公共卫生相关性：我们要求一个CCD摄像机，用于记录电子显微镜中的数字图像。显微镜设施致力于研究生物大分子的3D结构。这些蛋白质复合物在人类健康和疾病中起着关键作用。大画幅相机及其相关软件将实现显微镜自动化，并显着提高显微镜的吞吐量和研究生产力。