CORRELATING LARGE-SCALE SERIAL EM FROM ULTRATHIN ATLUM SECTIONS AND SERIAL E

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这个合作项目的目标是，首先也是最重要的，通过哈佛大学的利希特曼实验室和NCMIR正在开发的方法，整合在重叠尺度上获得的重建数据，使用类似的大脑皮质突触神经束的子体积来建立关于特定大脑区域连接的信息的连续性，以及关于突触前和突触后微结构的3D细节。这将通过将使用原型胶带车床切片机(哈佛大学)生产的薄片的背向散射电子成像获得的信息与来自连续切片电子显微镜(EM)断层摄影术(在NCMIR)的子体积相结合来完成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goals of this collaborative project are, first and foremost, to integrate reconstruction data taken at overlapping scales by methods being developed by the Lichtman laboratory at Harvard and by NCMIR using a similar subvolume of cerebral cortex synaptic neuropil to establish continuity of information about the wiring of a specific brain region¿¿with 3D details about presynaptic and postsynaptic microstructures. This will be done by merging information obtained by backscatter electron imaging of thin sections produced using a prototype taping lathe microtome (at Harvard) with subvolumes from serial section electron microscopic (EM) tomography (at NCMIR).