CORRELATING LARGE-SCALE SERIAL EM FROM ULTRATHIN ATLUM SECTIONS AND SERIAL E

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 该合作项目的目标首先是整合通过哈佛的Lichtman实验室和NCMR开发的方法在重叠尺度上获得的重建数据，使用大脑皮层突触神经元的类似子体积来建立关于特定大脑区域布线的信息的连续性提供关于突触前和突触后微观结构的3D细节。这将通过合并使用原型胶带车床切片机（哈佛）制作的薄切片的背散射电子成像获得的信息与来自连续切片电子显微镜（EM）断层扫描（NCMIR）的子体积来完成。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goals of this collaborative project are, first and foremost, to integrate reconstruction data taken at overlapping scales by methods being developed by the Lichtman laboratory at Harvard and by NCMIR using a similar subvolume of cerebral cortex synaptic neuropil to establish continuity of information about the wiring of a specific brain region¿¿with 3D details about presynaptic and postsynaptic microstructures. This will be done by merging information obtained by backscatter electron imaging of thin sections produced using a prototype taping lathe microtome (at Harvard) with subvolumes from serial section electron microscopic (EM) tomography (at NCMIR).