ELECTROSTATIC PROPERTIES OF CCMV AND CMV

CCMV和CMV的静电特性

• 批准号: 7955247
• 负责人: CHARLES L BROOKS
• 金额: $ 0.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955247
• 关键词: Antiviral Agents; Biological; Biological Models; Biomedical Computing; Bromoviridae; Capsid; Capsid Proteins; Cells; Computer Retrieval of Information on Scientific Projects Database; Cytomegalovirus; Electrostatics; Family; Funding; Genetic Materials; Grant; Institution; Life Cycle Stages; Plant RNA; Process; Property; Proteins; Research; Research Personnel; Resources; Source; Structure; Therapeutic; United States National Institutes of Health; Viral; Virus; base; cowpea chlorotic mottle virus; design; insight; research study; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Assembly and disassembly of a viral capsid, or a protein shell, are essential steps in the life cycle of a virus. Understanding of viral assembly/disassembly can provide additional insights to other oligomerization processes as in protein association in large cellular assemblies, which occurs through a similar mechanism. Additionally, understanding the properties and function of the capsid shell alone is important for inferring all steps involved in host cell entry and release of the enveloped genetic material and can aid in proposing efficient ways of interfering with capsid assembly and stability via structure-based design of antiviral therapeutics. One of the most intensely studied viruses has been the cowpea chlorotic mottle virus (CCMV), a positive strand RNA plant-infecting virus which belongs to the Bromoviridae family. It is composed of a protein capsid and viral RNA which is packed inside. It has been widely used as a model system for understanding of biological assembly because of its icosahedral structure and the limited number of gene products required for its assembly. CCMV offers an accessible model system for examining the processes that regulate viral assembly, disassembly, and stability, both for theoretical and experimental studies.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 病毒衣壳或蛋白质外壳的组装和拆卸是病毒生命周期中的重要步骤。了解病毒组装/拆卸可以提供额外的见解，其他寡聚化过程中的蛋白质协会在大细胞组件，这是通过类似的机制发生。此外，理解衣壳壳的性质和功能对于推断宿主细胞进入和包膜遗传物质释放中涉及的所有步骤是重要的，并且可以帮助提出通过基于结构的抗病毒治疗设计干扰衣壳组装和稳定性的有效方法。 研究最深入的病毒之一是豇豆褪绿斑驳病毒（CCMV），一种正链RNA植物感染病毒，属于溴病毒科。它由蛋白质衣壳和包装在里面的病毒RNA组成。由于其二十面体结构和有限的基因产物数量，它已被广泛用作理解生物组装的模型系统。CCMV为理论和实验研究提供了一个可访问的模型系统，用于检查调节病毒组装、拆卸和稳定性的过程。