Core E: Protein

礼来公司圣地亚哥生物技术中心位于一个专门建造的12,000平方英尺的实验室内，配备了所有必要的仪器/计算机，包括10个温度控制的摇床培养箱(每天可发酵180升)，4个4通道AKT Axpress色谱机器人加上13个单通道Akta色谱系统，应用生物系统MALDI-TOF(Voyager DE-RP和Voyager DE-STR)，应用生物系统ESI-四极杆质谱仪，ThermoFinnigan LCQ LCQ Deca XP Plus用于MS/MS分析的ESI-LontraTrap质谱仪。应用生物系统QTrap 4000质谱仪、5台安捷伦1100 HPLC、Wyatt Minidawn Treso多角度光散射和OptLab 这些设备包括折射率探测器系统、AVIV 215型CD光谱仪、Biacore T100、Robbins Phoenix结晶机器人和用于准备优化托盘的Formulatrix配方机器人。 其余50%的蛋白质结晶将在AECOM进行，类似地，在专门建造的 配备了结晶实验室。 X射线衍射筛选和数据收集将在APS使用礼来公司最先进的LRL-CAT插入设备光束线进行，该设备配备了马赛克CCD区域探测器和低温晶体处理/安装机器人。 在APS维护关闭期间(日历年的-25%)，访问位于 国家同步加速器光源将允许不间断地访问X射线数据收集设施，并偶尔有机会进行专家干预，以挑战衍射数据收集(根据需要)。

Eli Lilly San Diego Center for Biotechnology in a purpose-built 12,000 square foot laboratory, equipped with all of the necessary instrumentation/computers, including 10 Temperature Controlled Shaker Incubators (capable of fermenting 180 liters per day), four 4-channel AKTAxpress Chromatography Robots plus 13 single channel AKTA Chromatography Systems, Applied Biosystems MALDI-TOF (Voyager DE-RP and Voyager DE-STR) Mass Spectrometers, Applied Biosystems ESI-Quadrupole Mass Spectrometer, ThermoFinnigan LCQ Deca XP Plus ESI-lontrap Mass Spectrometer for MS/MS analysis. Applied Biosystems Qtrap 4000 Mass Spectrometer, five Agilent 1100 HPLCs, Wyatt Minidawn Treso Multiangle Light Scattering and Optlab Refractive Index Detector System, an Aviv Model 215 CD Spectrometer, a Biacore T100, a Robbins Phoenix Crystallization Robot, and a Formulatrix Formulator robot for preparing optimization trays. The remaining 50% of protein crystallization will be conducted at AECOM in a purpose built, similarly equipped crystallization laboratory. X-ray diffraction screening and data collection will be conducted at the APS using Lilly's State-of-the-Art LRL-CAT insertion device beamline, which is equipped with a mosaic CCD area detector and cryogenic crystal handling/mounting robotics. During APS maintenance shutdowns (-25% of the calendar year), access to Beamline X29 at the National Synchrotron Light Source will permit uninterrupted access to X-ray data collection facilities and occasional opportunities for expert intervention for challenging diffraction data collection (as needed).