Core E: Protein

核心E：蛋白质

• 批准号: 7980200
• 负责人: JOHN A GERLT
• 金额: $ 167.1万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7980200
• 关键词: Area; Arts; Automation; Biotechnology; Calendar; Chromatography; Computers and Advanced Instrumentation; Core Protein; Crystallization; Data; Data Collection; Databases; Deposition; Devices; E protein; Enzymes; Gene Targeting; Generations; Incubators; Intervention; Laboratories; Light; Maintenance; Medicine; Methods; Modeling; National Institute of General Medical Sciences; New York; Pharmacologic Substance; Photons; Production; Protein Structure Initiative; Proteins; Reagent; Refractive Indices; Research; Robot; Robotics; Roentgen Rays; Scientist; Screening procedure; Source; Synchrotrons; System; Temperature; United States National Institutes of Health; X ray diffraction analysis; X-Ray Diffraction; beamline; college; cryogenics; design; detector; light scattering; mass spectrometer; square foot; structural biology; structural genomics; web site

Eli Lilly San Diego Center for Biotechnology in a purpose-built 12,000 square foot laboratory, equipped with all of the necessary instrumentation/computers, including 10 Temperature Controlled Shaker Incubators (capable of fermenting 180 liters per day), four 4-channel AKTAxpress Chromatography Robots plus 13 single channel AKTA Chromatography Systems, Applied Biosystems MALDI-TOF (Voyager DE-RP and Voyager DE-STR) Mass Spectrometers, Applied Biosystems ESI-Quadrupole Mass Spectrometer, ThermoFinnigan LCQ Deca XP Plus ESI-lontrap Mass Spectrometer for MS/MS analysis. Applied Biosystems Qtrap 4000 Mass Spectrometer, five Agilent 1100 HPLCs, Wyatt Minidawn Treso Multiangle Light Scattering and Optlab Refractive Index Detector System, an Aviv Model 215 CD Spectrometer, a Biacore T100, a Robbins Phoenix Crystallization Robot, and a Formulatrix Formulator robot for preparing optimization trays. The remaining 50% of protein crystallization will be conducted at AECOM in a purpose built, similarly equipped crystallization laboratory. X-ray diffraction screening and data collection will be conducted at the APS using Lilly's State-of-the-Art LRL-CAT insertion device beamline, which is equipped with a mosaic CCD area detector and cryogenic crystal handling/mounting robotics. During APS maintenance shutdowns (-25% of the calendar year), access to Beamline X29 at the National Synchrotron Light Source will permit uninterrupted access to X-ray data collection facilities and occasional opportunities for expert intervention for challenging diffraction data collection (as needed).

礼来圣地亚哥生物技术中心位于专门建造的 12,000 平方英尺实验室中，配备了所有必要的仪器/计算机，包括 10 个温控摇床培养箱（每天能够发酵 180 升）、四个 4 通道 AKTAxpress 色谱机器人以及 13 个单通道 AKTA 色谱系统、Applied Biosystems MALDI-TOF（Voyager DE-RP 和 Voyager DE-STR) 质谱仪、Applied Biosystems ESI-四极杆质谱仪、ThermoFinnigan LCQ Deca XP Plus ESI-lontrap 质谱仪用于 MS/MS 分析。 Applied Biosystems Qtrap 4000 质谱仪、五台 Agilent 1100 HPLC、Wyatt Minidawn Treso 多角度光散射和 Optlab 用于准备优化托盘的示差折光检测器系统、Aviv Model 215 CD 光谱仪、Biacore T100、Robbins Phoenix 结晶机器人和 Formulatrix Formulator 机器人。 其余 50% 的蛋白质结晶将在 AECOM 专门建造的实验室中进行，类似地 配备结晶实验室。 X 射线衍射筛选和数据收集将在 APS 中使用礼来公司最先进的 LRL-CAT 插入设备光束线进行，该光束线配备了镶嵌 CCD 区域探测器和低温晶体处理/安装机器人。 在 APS 维护停机期间（日历年的 -25%），可以在以下位置访问 Beamline X29： 国家同步加速器光源将允许不间断地访问 X 射线数据收集设施，并偶尔有机会为具有挑战性的衍射数据收集提供专家干预（根据需要）。