Core E: Protein

Eli Lilly San Diego Center for Biotechnology位于一个专门建造的12，000平方英尺的实验室中，配备了所有必要的仪器/计算机，包括10个温控振荡培养箱（每天发酵180升），四个4通道AKTAxpress色谱机器人加上13个单通道AKTA色谱系统，Applied Biosystems MALDI-TOF（Voyager DE-RP和Voyager DE-STR）质谱仪、Applied Biosystems ESI-四极杆质谱仪、ThermoFinnigan LCQ Deca XP Plus ESI-离子阱质谱仪用于MS/MS分析。Applied Biosystems Qtrap 4000质谱仪、5台Agilent 1100 HPLC、Wyatt Minidawn Treso多角度光散射和Optlab 折射率检测器系统、Aviv 215型CD光谱仪、Biacore T100、Robbins Phoenix结晶机器人和Formulatrix Formulator机器人用于制备优化托盘。 其余50%的蛋白质结晶将在AECOM专门建造的类似设施中进行， 配备结晶实验室。 将在APS使用礼来最先进的LRL-CAT插入装置光束线进行X射线衍射筛选和数据收集，该光束线配备有镶嵌CCD区域探测器和低温晶体处理/安装机器人。 在APS维护停机期间（日历年的-25%）， 国家同步加速器光源将允许不间断地访问X射线数据收集设施，并偶尔有机会为挑战衍射数据收集提供专家干预（如需要）。

Eli Lilly San Diego Center for Biotechnology in a purpose-built 12,000 square foot laboratory, equipped with all of the necessary instrumentation/computers, including 10 Temperature Controlled Shaker Incubators (capable of fermenting 180 liters per day), four 4-channel AKTAxpress Chromatography Robots plus 13 single channel AKTA Chromatography Systems, Applied Biosystems MALDI-TOF (Voyager DE-RP and Voyager DE-STR) Mass Spectrometers, Applied Biosystems ESI-Quadrupole Mass Spectrometer, ThermoFinnigan LCQ Deca XP Plus ESI-lontrap Mass Spectrometer for MS/MS analysis. Applied Biosystems Qtrap 4000 Mass Spectrometer, five Agilent 1100 HPLCs, Wyatt Minidawn Treso Multiangle Light Scattering and Optlab Refractive Index Detector System, an Aviv Model 215 CD Spectrometer, a Biacore T100, a Robbins Phoenix Crystallization Robot, and a Formulatrix Formulator robot for preparing optimization trays. The remaining 50% of protein crystallization will be conducted at AECOM in a purpose built, similarly equipped crystallization laboratory. X-ray diffraction screening and data collection will be conducted at the APS using Lilly's State-of-the-Art LRL-CAT insertion device beamline, which is equipped with a mosaic CCD area detector and cryogenic crystal handling/mounting robotics. During APS maintenance shutdowns (-25% of the calendar year), access to Beamline X29 at the National Synchrotron Light Source will permit uninterrupted access to X-ray data collection facilities and occasional opportunities for expert intervention for challenging diffraction data collection (as needed).