DEVELOPMENT OF SPECIFIC MICROSPHERE-BASED PROTEASE ASSAYS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项合作是为了开发高通量蛋白酶测定法，用于UNM的Hypercyte平台。 在LANL，我们正在开发基于微球的蛋白酶检测，该检测使用附着在微球上的荧光底物，并通过常规流式细胞术进行分析。 在UNM，我们正在将这些检测纳入Hypercyte平台，用于高通量测量和化学文库筛选。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This collaboration is an effort to develop high throughput protease assays for use on the Hypercyte platform at UNM. At LANL we are developing microsphere based protease assays that use fluorescent substrates attached to microspheres and analyzing them by conventional flow cytometry. At UNM we are incorporating those assays into the Hypercyte platform for high throughput measurements and chemical library screening.