DEVELOPMENT OF A HAND-HELD FLOW CYTOMETER

手持式流式细胞仪的开发

• 批准号: 8361759
• 负责人: STEVEN W GRAVES
• 金额: $ 4.16万
• 依托单位: LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361759
• 关键词: Area; Biological; Biomedical Research; Detection; Development; Diagnostic; Flow Cytometry; Forensic Medicine; Funding; Genotype; Goals; Grant; HIV diagnosis; Hand; Laboratories; Ligands; Liquid substance; Measurement; Medicine; National Center for Research Resources; Optics; Performance; Pharmacologic Substance; Phase; Physiologic Thermoregulation; Principal Investigator; Research; Research Infrastructure; Research Personnel; Resources; Sampling; Sorting - Cell Movement; Source; System; Techniques; Technology; United States National Institutes of Health; Universities; World Health; cancer diagnosis; cost; data acquisition; high throughput screening; instrument; medical schools; molecular assembly/self assembly; new technology; particle; portability; receptor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to develop low cost portable flow cytometers for use in biomedical applications. Such instruments would have a large impact in many areas including medicine (HIV and cancer diagnosis), homeland defense (biological point detection, bio-surveillance, and forensic analysis) and general biomedical research (ligand-receptor studies, molecular assembly analysis, high throughput screening and genotyping). The many powerful applications have made flow cytometers a fixture in nearly every university, medical school, pharmaceutical company, and diagnostic lab. However, the size, expense and requirement for large amounts of consumables have limited their use to formal laboratory settings. A low cost portable flow cytometer would make these applications available to all clinicians and researchers, which would have a great impact on world health and research. To develop such an instrument we will develop (in the R21 phase) technologies that allow particle focusing without the use of sheath fluid. The elimination of sheath fluid will result in increased portability of the instrument and also reduce consumable requirements. Our novel technology will allow a sheathless portable flow cytometer to achieve conventional particle analysis rates, while using a fraction of the power and consumables of a conventional flow cytometer. We will also develop low power excitation/detection systems (R21 phase) that will not require extensive thermoregulation to achieve optical measurement stability. Such systems, will significantly reduce the size, cost and power requirements of a flow cytometer. Finally, we will integrate the above technologies into a miniature affordable flow cytometer, with advanced sample handling capabilities that has significantly reduced consumable and power requirements (R33 phase). To do this we will combine the above technologies into a microfabricated flow cytometer core that can easily be interfaced with sample handling and optical modules. We will also develop a miniature low power data acquisition system to enable assembly of a complete miniature low power flow cytometer. We will validate instrument performance using quantitative flow cytometry techniques.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的目的是开发用于生物医学应用的低成本便携式流式细胞仪。这些仪器在许多领域都会产生很大的影响，包括医学（HIV和癌症诊断），国土防御（生物学分检测，生物救生和法医分析）和一般生物医学研究（配体受体研究，分子装配分析，高吞吐量筛查和基因分型）。许多强大的应用程序使流式细胞仪几乎是每个大学，医学院，制药公司和诊断实验室的固定装置。 但是，大量消耗品的规模，费用和要求限制了它们对正式实验室环境的用途。 低成本的便携式流式细胞仪将使所有临床医生和研究人员都可以使用这些应用，这将对世界健康和研究产生重大影响。 为了开发这样的仪器，我们将开发（在R21相）的技术（在R21相中），该技术允许在不使用护套流体的情况下进行颗粒聚焦。 消除护套液将导致仪器的可移植性增加，并减少消耗量的要求。我们的新技术将允许无鞘的便携式流式细胞仪达到传统的粒子分析速率，同时使用常规流式细胞仪的功率和消耗量的一部分。 我们还将开发低功率激发/检测系统（R21相），该系统不需要大量的温度调节即可实现光学测量稳定性。这样的系统将大大降低流式细胞仪的大小，成本和功率要求。 最后，我们将上述技术集成到微型负担得起的流式细胞仪中，并具有高级样品处理能力，这些功能大大降低了消耗量和功率要求（R33阶段）。 为此，我们将上述技术将上述技术结合到微型制作的流式细胞仪芯中，该芯可以很容易地与样品处理和光学模块相连。我们还将开发一个微型低功率数据采集系统，以使完整的微型低功率流式细胞仪组装。我们将使用定量流式细胞仪技术验证仪器性能。