SOLUTION X-RAY SCATTERING STUDIES ON THE CONFORMATIONAL CHANGE OF EUKARYOTIC AND

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 这个建议是为了延长我们早期的解决方案X射线散射研究真核伴侣TRiC更高的结构分辨率，使用改进的仪器和计算技术。该建议的另一个重点是将我们的研究扩展到archael伴侣Mm-cpn，另一个第二组伴侣。Mm-cpn可以大量纯化，因此更适合于时间分辨研究，以观察ATP水解循环期间的溶液结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This proposal is intended to extend our earlier solution x-ray scattering studies on eukaryotic chaperonine TRiC to a higher structural resolution using the improved instrumentation and computational techniques. The other focus of the proposal is to extend our research to the archael chaperonine Mm-cpn, another Group II chaperonine. Mm-cpn can be purified in a larger quantity, thus more suitable for time-resolved studies to look at the solution structure during the ATP hydrolysis cycle.