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Development of Nanoscale Plasmonic Devices for Creation of a Next Generation Surf

开发纳米级等离激元器件以创造下一代冲浪

基本信息

批准号：
8071591
负责人：
DAVID H WALDECK
金额：
$ 18.17万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-05-15 至 2013-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8071591
关键词：
Address Area Beryllium Binding Biochemical Phenomena Biological Biological Assay Biological Models Biological Monitoring Biological Process Biological Sciences Biomedical Research Cells Characteristics Chemicals Clinic Coupled Coupling Detection Development Devices Diagnostic Elements Event Film Foundations Generations Glycerol Growth Healthcare Investigation Learning Link Lipids Liquid substance Measurement Measures Medical Membrane Proteins Metabolic Pathway Metabolism Metals Methodology Methods Microfluidic Microchips Microfluidics Miniaturization Modification Monitor Nanoarray Analytical Device Nanotechnology Nature Optics Oxidation-Reduction Pathway interactions Physical Chemistry Play Preparation Process Property Protein Analysis Proteins Regulation Reporting Research Role Side Specificity Spectrum Analysis Structure Surface Surface Plasmon Resonance Technology Thick Time Translations Work analytical tool base biological research charge transfer complex design experience indexing insight instrument light transmission multiplex detection nanometer nanoscale nanostructured new technology next generation photonics plasmonics protein protein interaction public health relevance skills structural biology transmission process

项目摘要

DESCRIPTION (provided by applicant): Recent developments in the design and fabrication of nanometer scale optical devices offer a new technology for the monitoring of biological events. In particular, we propose to exploit these developments to create plasmonic sensing elements and demonstrate their utility for monitoring biological binding events. Because the sensing elements are small, just a few tens of microns on a side, they can be integrated with microfluidic devices to provide a platform for performing parallel and/or multiplex investigations. This technology promises to displace the conventional surface plasmon resonance (SPR) methodology for studying biological interactions, hence its creation and development will have a significant impact on biological and biomedical research. We will demonstrate the capabilities of this new technology by investigating aspects of glycerol metabolism and the specific protein-protein interactions that are essential in its regulation. Through rational design we will prepare specific chemical surface modification and use it to study aspects of this important biological pathway. However, we anticipate that the technology will be broader and by creating plasmonic devices that are easily integrated into microfluidic platforms we will create the fundamental technological foundation that is required to fully exploit plasmonic processes in sensing biological events, leading to a variety of applications in healthcare and life sciences research. This platform will make possible the creation of analytical tools for the preparation, detection, and analysis of proteins that are expressed at low abundance and/or transiently, obtained from biological fluids and cells. PUBLIC HEALTH RELEVANCE (provided by applicant): We are exploiting new developments in photonics and nanotechnology to create a new type of surface plasmon resonance (SPR) spectroscopy. Although conventional SPR is widely used in biomedical research, the proposed technology promises to dramatically grow that use in research and expand its use to medical diagnostics. Because this technology can be integrated with a microfluidic platform we will enable the creation of analytical tools for the preparation, detection, and analysis of proteins that are expressed at low abundance and/or transiently, obtained from biological fluids and cells.

描述（由申请人提供）：纳米级光学器件的设计和制造的最新发展为监测生物事件提供了一种新技术。特别是，我们建议利用这些发展来创建等离子体传感元件，并证明其用于监测生物结合事件的实用性。由于传感元件很小，只有几十微米的一面，它们可以与微流体设备集成，以提供一个平台，用于执行并行和/或多路调查。该技术有望取代传统的表面等离子体共振（SPR）方法来研究生物相互作用，因此它的创建和发展将对生物和生物医学研究产生重大影响。我们将通过研究甘油代谢的各个方面以及在其调节中必不可少的特定蛋白质-蛋白质相互作用来展示这种新技术的能力。通过合理的设计，我们将制备特定的化学表面改性，并使用它来研究这一重要的生物途径的各个方面。然而，我们预计该技术将更广泛，通过创建易于集成到微流体平台中的等离子体设备，我们将创建充分利用等离子体过程所需的基本技术基础，以传感生物事件，从而在医疗保健和生命科学研究中实现各种应用。该平台将使得有可能创建用于制备、检测和分析从生物流体和细胞获得的以低丰度和/或瞬时表达的蛋白质的分析工具。公共卫生相关性（由申请人提供）：我们正在利用光子学和纳米技术的新发展来创建一种新型的表面等离子体共振（SPR）光谱。虽然传统的SPR广泛用于生物医学研究，但所提出的技术有望在研究中显着增加这种用途，并将其应用扩展到医学诊断。由于这项技术可以与微流体平台集成，我们将能够创建用于制备、检测和分析从生物流体和细胞中获得的低丰度和/或瞬时表达的蛋白质的分析工具。