Urethral Microbiome of Adolescent Males

青春期男性的尿道微生物组

• 批准号: 8117351
• 负责人: J. Dennis Fortenberry
• 金额: $ 153.04万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8117351
• 关键词: Acute; Address; Adolescence; Adolescent; Adult; African American; Age; American; Anterior; Antibiotics; Anus; Area; Behavioral; Cellular Phone; Clinic; Clinical; Clinical Data; Data; Development; Enrollment; Epidemiology; Ethnic group; Event; Failure; Female Adolescents; Genital system; Health; High Prevalence; Immunologics; Infection; Infection prevention; Latino; Link; Maintenance; Male Adolescents; Male Circumcision; Male urethral structure; Normal Range; Oral; Organism; Participant; Phylogeny; Puberty; Public Health; Recruitment Activity; Research; Rest; Ribosomal RNA; Risk; Sampling; Schedule; Sex Behavior; Sexually Transmitted Diseases; Symptoms; Technology; Testing; Urethra; Urethritis; Urine; Vagina; Variant; cohort; condoms; design; follow-up; improved; male; men; metagenomic sequencing; microbial; microbial community; microbiome; public health relevance; racial and ethnic; sample collection

DESCRIPTION: The microflora of the anterior male urethra during adolescence is poorly described and no data address the range of "typical" urethral microflora during adolescence as functions of pubertal development or onset of various types of partnered sexual activity. This means that current understanding of the urethral microbiome in adolescent men rests entirely on data drawn from adults, and from epidemiologic and behavioral explanations. Marked ethnic group variation in adolescent sexual behaviors and disparity in conditions such as sexually transmitted infections (STI) justifies exploration of potential ethnic group differences in the male urethral microflora. Some research among adult males suggests important interactions between the male urethral microflora and STI, but this research has not been extended to younger men. Here we propose to characterize the male urethral microbiome in longitudinal urine samples collected from a multi-ethnic cohort of adolescent males. Microbial flora will be assessed as function of pubertal development, sexual activity, and incident STI. Subjects will be Latino (N=24), African-American (N=24), and Euro-American (N=24) males, ages 14 - 17 at enrollment; total N=72). Participants will be recruited from an urban clinic within an area of high prevalence of STI. Socio-demographic data will be collected at enrollment. Daily behavioral and symptomologic data will be collected via cell phone, which will also be used for cohort maintenance and scheduling of sample collections. Urine will be collected at enrollment and up to 36 1-month follow-up samples for characterization of microflora using high-throughput 16S rRNA phylogeny, Q-PCR and metagenomic sequencing. STI testing will be done as well to better understand interactions between the urethral microbiome and infection. Cell phones also will be used to initiate daily sample collection for 10 days following trigger events such as specific sexual acts, antibiotic use, condom failure, or genital symptoms. PUBLIC HEALTH RELEVANCE: The project is designed to describe the range of the normal microbiome of adolescent males, to describe the changes associated with puberty and to link the microbiome to potential health conditions such as sexually transmitted infections.

产品说明：青春期男性前尿道的微生物群落描述不多，没有数据说明青春期“典型”尿道微生物群落的范围与青春期发育或各种类型的伴侣性活动的发生有关。这意味着目前对青少年男性尿道微生物组的理解完全依赖于来自成年人的数据，以及流行病学和行为学解释。青少年性行为的明显种族差异和性传播感染（STI）等疾病的差异证明了探索男性尿道微生物群落的潜在种族差异是合理的。在成年男性中的一些研究表明，男性尿道微生物群落和性传播感染之间存在重要的相互作用，但这项研究尚未扩展到年轻男性。在这里，我们建议从多种族青少年男性队列中收集的纵向尿液样本中描述男性尿道微生物组。微生物植物群将作为青春期发育、性活动和STI事件的函数进行评估。受试者将为拉丁裔（N=24）、非洲裔美国人（N=24）和欧美裔（N=24）男性，入组时年龄为14 - 17岁;总计N=72）。参与者将从性传播感染高发地区的城市诊所招募。将在入组时收集社会人口统计学数据。将通过手机收集日常行为和病理学数据，这些数据也将用于队列维护和样本采集计划。将在入组时采集尿液，并采集长达36个1个月的随访样本，用于使用高通量16 S rRNA基因组学、Q-PCR和 宏基因组测序STI测试也将进行，以更好地了解尿道微生物组和感染之间的相互作用。在特定的性行为、抗生素使用、避孕套失效或生殖器症状等触发事件发生后，还将使用手机开始10天的每日样本收集。公共卫生关系：该项目旨在描述青少年男性正常微生物组的范围，描述与青春期相关的变化，并将微生物组与潜在的健康状况（如性传播感染）联系起来。