Tagging of Laboratory Mice with Microtransponders

用微转发器标记实验室小鼠

• 批准号: 8136100
• 负责人: WLODEK MANDECKI
• 金额: $ 66.65万
• 依托单位: PHARMASEQ, INC.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8136100
• 关键词: Acoustics; Address; Adhesives; Adoption; Aging; Algorithms; Amplifiers; Animal Welfare; Animals; Architecture; Back; Basic Science; Behavior assessment; Biocompatible; Biomedical Research; Boxing; Breeding; Characteristics; Collaborations; Computer software; Computers; Data; Databases; Dentistry; Detection; Development; Dimensions; Disease; Elements; Embryo; Evaluation; Excision; Feasibility Studies; Feedback; Frequencies; Fuzzy Logic; Genetic Research; Genetic Variation; Goals; Health; Housing; Human; Immunocompetent; Implant; Inherited; Investigation; Label; Laboratories; Laboratory Research; Laboratory mice; Lasers; Laws; Lead; Light; Liquid substance; Magnetism; Mammalian Genetics; Mechanics; Medicine; Metals; Modeling; Mouse Strains; Mus; Names; Needles; Neurosciences; New Jersey; Nitrogen; Noise; Pathologic; Pattern; Pennsylvania; Pharmacologic Substance; Phase; Phenotype; Play; Procedures; Process; Radio; Reader; Reading; Recording of previous events; Records; Research; Rivers; Robot; Role; Scheme; Schools; Semiconductors; Shapes; Signal Transduction; Silicon; Skin; Sorting - Cell Movement; Staging; Surface; System; Tail; Techniques; Testing; Thick; Time; Transistors; United States National Institutes of Health; Universities; Veterinary Medicine; Width; Work; analog; animal breeding; attenuation; base; biomaterial compatibility; computerized data processing; cost; design; detector; digital; immunosuppressed; improved; new technology; oncology; operation; parylene; prevent; programs; public health relevance; sensor; software systems

DESCRIPTION (provided by applicant): The purpose of the present project is to develop a new type of RFID tagging system for tracking mice. It is based on the PharmaSeq laser light-activated microtransponder, also known as "p-Chip". Its major advantages are its small size (500 5m x 500 5m x 100 5m), monolithic (one part) design, inertness (silicon-based), ease of use and very low cost. No anesthetization of the mice is required. The system consists of the tag itself, the tag reader, and an injector for p-Chips. The p-Chips are rapidly implanted in a mouse's tail just under the skin. To identify the animal, the operator simply brings a reader to within the immediate proximity of the implanted p-Chip and the ID is acquired automatically and transmitted to the a computer. The software further associates the ID of a particular mouse with data related to its role in experimental procedures and/or breeding history. The main goals of the project are to: (1) make several improvements to the injector regarding the ease of use, and fabricate a larger number of units; (2) conduct animal studies including biocompatibility in immunocompetent as well as immunosuppressed strains of mice; (3) modify the existing p-Chip/ID reader system, including the digital board and software; and (4) redesign of p-Chip/receiver system to work at higher frequencies (315 MHz). The system will be thoroughly tested in-house and in collaboration with outside laboratories. Tagging with p-Chips will improve the reliability of tracking of experimental subjects by preventing costly errors including rework due do unreadable markings and lost labels. PUBLIC HEALTH RELEVANCE: Laboratory mice play a powerful role in basic research and a key role in mammalian genetic and biomedical research. They are universally accepted as the primary model for analyzing and understanding inherited human disorders. The new PharmaSeq system has the potential to greatly increase the use of RFID tags in laboratory mice, or even to lead to the general adoption of this new technology as a universal tagging system that can track animals from breeding through their entire useful lifetime.

描述（由申请人提供）：本项目的目的是开发一种用于跟踪老鼠的新型RFID标签系统。它基于 PharmaSeq 激光激活微应答器，也称为“p-Chip”。其主要优点是尺寸小（500 5m x 500 5m x 100 5m）、单片（一件式）设计、惰性（基于硅）、易于使用和成本非常低。不需要对小鼠进行麻醉。该系统由标签本身、标签读取器和 p-Chips 注入器组成。 p-芯片被快速植入小鼠尾巴的皮下。为了识别动物，操作员只需将读取器带到植入的 p-Chip 附近，ID 就会自动获取并传输到计算机。该软件进一步将特定小鼠的 ID 与其在实验程序和/或繁殖历史中的作用相关的数据关联起来。该项目的主要目标是：（1）在易用性方面对喷射器进行多项改进，并制造更多数量的单元； (2) 进行动物研究，包括免疫活性和免疫抑制小鼠品系的生物相容性； (3)修改现有的p-Chip/ID读卡器系统，包括数字板和软件； (4) 重新设计 p-Chip/接收器系统以在更高频率 (315 MHz) 下工作。该系统将在内部并与外部实验室合作进行彻底测试。使用 p-Chips 进行标记将通过防止代价高昂的错误（包括因标记不可读和标签丢失而导致的返工）来提高跟踪实验对象的可靠性。 公共卫生相关性：实验小鼠在基础研究中发挥着重要作用，在哺乳动物遗传和生物医学研究中发挥着关键作用。它们被普遍认为是分析和理解人类遗传性疾病的主要模型。新的 PharmaSeq 系统有可能大大增加 RFID 标签在实验室小鼠中的使用，甚至导致这种新技术作为通用标签系统的普遍采用，可以跟踪动物从繁殖到整个使用寿命的过程。

项目成果

A system for implanting laboratory mice with light-activated microtransponders.

一种为实验室小鼠植入光激活微应答器的系统。

• 发表时间: 2010
• 期刊: Journal of the American Association for Laboratory Animal Science : JAALAS
• 作者: Gruda,MaryannC;Pinto,Amanda;Craelius,Aaron;Davidowitz,Hanan;Kopacka,WesleyM;Li,Ji;Qian,Jay;Rodriguez,Efrain;Kuspiel,Edward;Mandecki,Wlodek
• 通讯作者: Mandecki,Wlodek