Broad Detection System for Tick-Borne Pathogens

蜱传病原体的广泛检测系统

• 批准号: 8111239
• 负责人: STEVEN E SCHUTZER
• 金额: $ 96.96万
• 依托单位: BIOSCIENCE DEVELOPMENT, INC.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8111239
• 关键词: Alphaproteobacteria; Anaplasma; Babesia; Bacillus anthracis spore; Bacteria; Bartonella; Biological Assay; Bite; Blood; Blood Volume; Borrelia; Borrelia burgdorferi; Businesses; Cells; Cerebrospinal Fluid; Clinical; Coupled; Coxiella; Cytolysis; DNA; Detection; Development; Diagnostic; Diagnostic Services; Diagnostic tests; Disease; Ehrlichia; Family; Flavivirus; Foundations; Francisella; Future; Gammaproteobacteria; Genome; Genomics; Goals; Health; Health system; Human; Incidence; Individual; Investigation; Ixodes; Joints; Liquid substance; Lyme Disease; Mass Spectrum Analysis; Mechanics; Medical; Methodology; Methods; Microbe; Modeling; Mollicutes; Nucleic Acids; Order Spirochaetales; Organism; Pathogen detection; Pathway interactions; Patients; Peptides; Phase; Physicians; Population Surveillance; Positioning Attribute; Protocols documentation; Protozoa; Public Health; RNA; Reaction; Reference Standards; Research; Research Personnel; Resistance; Rickettsia; Sampling; Skin; Specimen; Spiroplasma; System; Testing; Tick-Borne Diseases; Ticks; Time; United States; Validation; base; clinically relevant; cost; improved; instrument; meetings; member; microbial; pathogen; success; tool; vector

DESCRIPTION (provided by applicant): There is an increasing number of cases and spread of Lyme and other tick-borne diseases in the United States. The same tick that can transmit the agent of Lyme disease, Borrelia burgdorferi, can simultaneously transmit other pathogens such as Babesia, Anaplasma, and Rickettsia. This is of great concern to the public and their physicians. This concern is reflected in a greater demand for tests that can provide information as to what is in an individual tick and what can be transmitted. Current diagnostic testing methods for these disease causing pathogens are generally limited to a single microbe and/or unreliable. No test exists that can cover the breadth of pathogens transmitted by ticks. In our Phase I application, we achieved our aim, the proof of concept, that our methodology could detect multiple microbes and even in a single tick. That system employs broad-range PCR and mass spectroscopy (PCR/ESI-MS). The assay identified and speciated broad groups of bacteria, including known tick- borne bacteria and flaviviruses. The success of this, coupled with the increasing demand and increasing number of searches performed for Borrelia alone, is the basis for Phase 2 to concentrate on the development of a tick-borne pathogen detection assay using the high throughput PCR/ESI-MS. In Phase 1, the proof of principle of detection of pathogens was accomplished with 16 primer pairs in 16 wells of a 96 well kitted plate. In Phase 2, Goal 1 of this is to increase clinically useful throughput time and efficiency of the assay and consequently reduce costs. We will have a target goal of 8 wells (PCR reactions) by eliminating redundancy and multiplexing. Goal 2: Optimize and add to these, Babesia detection primers. Goal 3: Create a replenishable bank of nucleic acid extraction from infected ticks for future investigation of this type of assay as well as tick-human interface research. This bank will be of commercial value. Upon the completion of Phase2, we will be in a position to provide a tick-microbial diagnostic service for researchers, physicians, public health entities, private and individual citizen sectors. This will be an important tool in management of Lyme and related tick-borne diseases for improved medical treatment and public health surveillance. It will also be the foundation for doing the same in patient skin, blood, joint fluid, and cerebrospinal fluid. PUBLIC HEALTH RELEVANCE: The ability to accurately identify multiple pathogens in the ticks capable of causing Lyme disease and related disorders will be important in management strategy of patients exposed or bitten by ticks. It will help reduce rising costs to the patient and the public health system.

描述（由申请人提供）：在美国，莱姆和其他tick传播疾病的病例越来越多。可以传递莱姆病药物的相同壁虱可以同时传输其他病原体，例如贝贝西亚，阿纳普拉斯菌和立克。这对公众及其医师来说是非常关注的问题。这种担忧反映在对测试的更大需求中，这些测试可以提供有关单个tick中什么以及可以传输的信息的信息。这些疾病引起病原体的当前诊断测试方法通常仅限于单个微生物和/或不可靠。没有测试可以涵盖壁虱传播的病原体的广度。在我们的I阶段应用中，我们实现了我们的目标，即我们的方法可以检测到多个微生物，甚至在一个tick中。该系统采用宽范围的PCR和质谱（PCR/ESI-MS）。该测定法确定并指定了广泛的细菌组，包括已知的壁虱传播细菌和黄病毒。这一成功，再加上对伯氏菌的需求的增加和搜索数量的增加，是第2阶段使用高吞吐量PCR/ESI-MS专注于开发tick传播病原体检测试验的基础。在第1阶段，在16个96个良好套装板的16口孔中，通过16个底漆对来检测病原体的原理证明。在第2阶段，目标1是增加临床上有用的吞吐时间和测定的效率，从而降低成本。我们将通过消除冗余和多路复用来实现8个井（PCR反应）的目标目标。目标2：优化并添加到这些，Babesia检测引物。目标3：从受感染的壁虱中创建可补充的核酸提取库，以将来研究这种类型的测定法以及tick人 - 人类界面研究。该银行将具有商业价值。阶段2完成后，我们将有能力为研究人员，医师，公共卫生实体，私人和个人公民部门提供tick-微生物诊断服务。这将是管理莱姆和相关tick传播疾病的重要工具，以改善医疗和公共卫生监测。这也将是在患者皮肤，血液，关节液和脑脊液中做同样的基础。公共卫生相关性：能够准确识别能够引起莱姆病和相关疾病的tick中多种病原体的能力在暴露或被tick虫咬伤的患者的管理策略中很重要。它将有助于降低患者和公共卫生系统的上升成本。

项目成果

Prevalence of Borrelia miyamotoi in Ixodes ticks in Europe and the United States.

• DOI: 10.3201/eid2010.131583
• 发表时间: 2014-10
• 期刊: Emerging infectious diseases
• 影响因子: 11.8
• 作者: Crowder CD;Carolan HE;Rounds MA;Honig V;Mothes B;Haag H;Nolte O;Luft BJ;Grubhoffer L;Ecker DJ;Schutzer SE;Eshoo MW
• 通讯作者: Eshoo MW

Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic.

• DOI: 10.1093/femsec/fix129
• 发表时间: 2017-11-01
• 期刊: FEMS microbiology ecology
• 影响因子: 4.2
• 作者: Honig V;Carolan HE;Vavruskova Z;Massire C;Mosel MR;Crowder CD;Rounds MA;Ecker DJ;Ruzek D;Grubhoffer L;Luft BJ;Eshoo MW
• 通讯作者: Eshoo MW

Survey of Ixodes pacificus Ticks in California Reveals a Diversity of Microorganisms and a Novel and Widespread Anaplasmataceae Species.

• DOI: 10.1371/journal.pone.0135828
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Eshoo MW;Carolan HE;Massire C;Chou DM;Crowder CD;Rounds MA;Phillipson CA;Schutzer SE;Ecker DJ
• 通讯作者: Ecker DJ