Electrophoretic Plasmonic Nanopore Genome Sequencer

电泳等离子纳米孔基因组测序仪

• 批准号: 8897688
• 负责人: EDGAR A MENDOZA
• 金额: $ 22.29万
• 依托单位: REDONDO OPTICS, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-19 至 2016-03-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8897688
• 关键词: Base Pairing; Biomedical Engineering; Cellular Phone; Collaborations; Custom; DNA; DNA Sequence; Deposition; Devices; Diploidy; Electromagnetic Fields; Engineering; Ensure; Fractals; Generations; Genes; Genome; Genomics; Goals; Hour; Human Genome; Image; Imagery; Imaging Techniques; Individual; Internet; Label; Lasers; Liquid substance; Marketing; Massive Parallel Sequencing; Medicine; Metals; Motion; National Human Genome Research Institute; New Mexico; Optics; Phase; Process; Pump; Raman Spectrum Analysis; Reading; Research; Resolution; Sampling; Scheme; Semiconductors; Silicon Dioxide; Single-Stranded DNA; Site; Stretching; Structure; Surface; System; Techniques; Technology; Thick; Time; Universities; Work; atomic layer deposition; base; biochip; biological research; colloidal nanoparticle; commercialization; cost; cost effective; density; design; ds-DNA; experience; human genome sequencing; innovation; instrumentation; nanochannel; nanofabrication; nanofluidic; nanomaterials; nanoparticle; nanopattern; nanopore; next generation sequencing; novel; optical imaging; photonics; plasmonics; programs; prototype; public health relevance; self assembly; tool

 DESCRIPTION (provided by applicant): The goal of this proposal is to develop a convenient and portable electrophoretic plasmonic nanopore (EPN-GeneS(tm)) DNA sequencing system that will enable the rapid, reliable, and automated DNA sequencing of an entire human genome within 30-minutes at a cost ($100) that will revolutionize biological research and medicine. The fundamental innovation is based on an electrophoretic plasmonic nanopore system (= 1.0-nm) use to slow and control the translocation rate of single stranded DNA molecules over wide ranges to enable accurate, fast throughput, massively parallel, and real time single base pair optical recognition. The propose EPN-GeneS(tm) sequencer uses a cost-effective and disposable fully integrated electrophoretic plasmonic nanopore (EPN) biochip that is interrogated via a custom "Smart" cell-phone attachment device that integrates opto-fluidics for electrophoretic fluid motion control, standard multi-wavelength semiconductor laser-diodes (pump & stokes) to excite the vibrational modes of the translocating DNA, and label-free visualization of the scattered Raman spectral signature of the DNA base-pair sequence using holographic filters integrated to the high resolution cell phone camera to acquire and process the sequence images via web connectivity, resulting in a cost affordable ($100) DNA sequencing device. In Phase I we will demonstrate the capability to control translocation rate of long DNA, i.e., Lambda-DNA, molecule through the fractal plasmonic nanoporous structures to enable the real time recognition of the base pair DNA sequence using optical imaging techniques. In Phase II we will optimize and integrate the EPN-GeneS(tm) nanofluidic biochips to achieve a cost affordable ($100) high quality and reliable complete DNA sequencing device.

 描述(由申请人提供)：这项提案的目标是开发一种方便和便携的电泳等离子纳米孔(EPN-gene(Tm))DNA测序系统，该系统将能够在30分钟内以100美元的成本对整个人类基因组进行快速、可靠和自动化的DNA测序，这将给生物研究和医学带来革命性的变化。根本的创新是基于一种电泳等离子体纳米孔系统(=1.0-nm)，用于在很大范围内减缓和控制单链DNA分子的移位速率，以实现准确、快速的吞吐量、大规模并行和实时的单碱基对光学识别。建议的EPN-gene(Tm)测序仪使用具有成本效益和一次性的全集成电泳性等离子体纳米孔(EPN)生物芯片，通过定制的“智能”手机附件设备进行查询，该设备集成了用于电泳液运动控制的光流体、标准的多波长半导体激光二极管(泵浦和斯托克斯)来激发移位DNA的振动模式，以及使用集成到高分辨率手机相机中的全息滤光片来采集和处理序列图像的DNA碱基序列的散射拉曼光谱特征的无标记可视化，从而产生了成本可承受的DNA测序设备(100美元)。在第一阶段，我们将展示通过分形等离子体纳米孔结构来控制长DNA，即Lambda-DNA分子的移位速率的能力，从而能够使用光学成像技术实时识别碱基对DNA序列。在第二阶段，我们将优化和集成EPN-gene(Tm)纳米流体生物芯片，以实现成本可承受(100美元)、高质量和可靠的完整DNA测序设备。