Phase Contrast Tomographic X-Ray Microscope System

相差断层X射线显微镜系统

• 批准号: 8734930
• 负责人: Peter Stephen Conti
• 金额: $ 111.13万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-06 至 2016-04-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8734930
• 关键词: Attenuated; Biological; Bone Development; Bone remodeling; Cardiology; Cardiovascular system; Computer software; Defect; Dentistry; Developmental Biology; Discipline; Disease; Engineering; Face; Funding; Genetic Medicine; Goals; Heart Diseases; Image; Medicine; Methods; Microscope; Minor; Neurosciences; Odontogenesis; Osteoporosis; Pathogenesis; Pathology; Pharmacologic Substance; Phase; Process; Research; Research Personnel; Resolution; Roentgen Rays; Science; Secure; Spatial Distribution; Speed; Stem cells; Structure; System; Three-Dimensional Image; Three-Dimensional Imaging; Tissues; United States National Institutes of Health; Work; X ray microscopy; X-Ray Tomography; base; bone; cardiogenesis; cost; improved; molecular imaging; novel; oncology; programs; skeletal regeneration; tissue regeneration; trait

DESCRIPTION (provided by applicant): The goal of this proposal is to secure funds to purchase a high resolution 3D X-Ray Tomography Microscope System, ZEISS Xradia 520 Versa (Carl Zeiss X-ray Microscopy Inc, formerly Xradia, Inc), equipped with a high speed workstation and analysis software. The addition of ZEISS Xradia 520 Versa at USC will facilitate the research of many of our investigators whose projects are aimed at understanding the processes that govern dental, bone, and heart development with a focus on developing novel tissue regeneration methods for fixing oro- and cranio-facial defects and improved understanding of bone remodeling for skeletal regeneration. In this proposal we highlight how the various features of the requested unit will enhance/aid the research of our users, with respect to their currently funded NIH research programs or in some cases, provide additional research capabilities. X-ray based 3D imaging has allowed investigators to quantify traits of attenuating biological tissues with a high degree of accuracy and precision in a non-destructive manner. Many of the bone and cardiovascular traits needed to advance our understanding of the pathogenesis of disease (heart disease, osteoporosis) are derived from the spatial distribution of structures (vessels, trabeculae) and are thus unique to 3D images. These traits cannot be readily determined from 2D images, unless one painstakingly reconstructs serial histological sections into a 3D stack. These histological studies are rare due to the prohibitively expensive cost and tremendous effort involved in generating and reconstructing the sections The addition of this system will strengthen ongoing work in engineering, dentistry, developmental biology, and genetic medicine by current users of the USC Molecular Imaging Center (MIC), as well as greatly enhance the ability of the Center to support new research in oncology, cardiology, pathology, material sciences, and developmental biology. We have gathered 15 NIH funded investigators (11 major and 4 minor users) with research disciplines in medicine, oncology, dentistry, pharmaceutical sciences, neurosciences, genetic medicine, engineering, stem cell, and developmental biology to support our current S10 application.

描述(由申请人提供)：本提案的目标是获得资金购买高分辨率3D X射线断层扫描显微镜系统，ZEISS XRadia 520 Versa(Carl Zeiss X射线显微镜公司，前身为XRadia，Inc.)，配备高速工作站和分析软件。ZEISS XRadia 520 Versa在南加州大学的加入将促进我们许多研究人员的研究，他们的项目旨在了解控制牙齿、骨骼和心脏发育的过程，重点是开发修复口腔和颅面部缺陷的新型组织再生方法，并提高对骨骼再生的骨重建的了解。在本提案中，我们重点介绍了所请求的单元的各种功能将如何加强/帮助我们的用户的研究，涉及他们目前资助的NIH研究项目，或者在某些情况下，提供额外的研究能力。基于X射线的3D成像使研究人员能够以非破坏性的方式以高精度和精确度量化衰减生物组织的特征。为了加深我们对疾病(心脏病、骨质疏松症)发病机制的理解，许多骨骼和心血管特征来自于结构(血管、骨小梁)的空间分布，因此是3D图像所独有的。这些特征不能很容易地从2D图像中确定，除非人们费力地将连续的组织切片重建成3D堆栈。这些组织学研究是罕见的，因为令人望而却步 该系统的加入将加强南加州大学分子成像中心(MIC)现有用户正在进行的工程、牙科、发育生物学和遗传医学方面的工作，并极大地增强该中心支持肿瘤学、心脏病学、病理学、材料科学和发育生物学新研究的能力。我们聚集了15名NIH资助的研究人员(11名主要用户和4名次要用户)，他们的研究学科包括医学、肿瘤学、牙科、药学、神经科学、遗传医学、工程学、干细胞和发育生物学，以支持我们目前的S10应用。

项目成果

Automated resolution independent method for comparing in vivo and dry trabecular bone

用于比较体内骨小梁和干燥骨小梁的自动分辨率独立方法

• DOI: 10.1002/ajpa.24181
• 发表时间: 2020
• 期刊: American Journal of Physical Anthropology
• 影响因子: 2.8
• 作者: Saers, Jaap P. P.;DeMars, Lily J.;Stephens, Nicholas B.;Jashashvili, Tea;Carlson, Kristian J.;Gordon, Adam D.;Ryan, Timothy M.;Stock, Jay T.
• 通讯作者: Stock, Jay T.

Combinations of trabecular and cortical bone properties distinguish various loading modalities between athletes and controls

• DOI: 10.1002/ajpa.24176
• 发表时间: 2020-11-27
• 期刊: AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY
• 影响因子: 2.8
• 作者: Saers, Jaap P. P.;DeMars, Lily J.;Stock, Jay T.
• 通讯作者: Stock, Jay T.