An Automated RNA 3D Structure Determination System

自动化 RNA 3D 结构测定系统

• 批准号: 8931349
• 负责人: Michael Edward Pape
• 金额: $ 72.86万
• 依托单位: NYMIRUM, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-05 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8931349
• 关键词: Cell physiology; Cells; Chemicals; Code; Complex; Computer software; Crystallography; Custom; Databases; Disease; Drug Industry; Encyclopedia of DNA Elements; Human Genome; Huntington Disease; Industry; Internet; Knowledge; Label; Licensing; Machine Learning; Malignant neoplasm of prostate; Measures; Methods; Molecular Biology; Myotonic Dystrophy; Oligonucleotides; Online Systems; Pharmaceutical Preparations; Phase; Publishing; RNA; Reporting; Research Personnel; Resolution; Ribonucleosides; Sales; Small Business Innovation Research Grant; Spinal Muscular Atrophy; Structure; Structure-Activity Relationship; System; Techniques; Technology; Testing; Time; Training; Untranslated RNA; Update; cloud based; human DNA; human disease; improved; innovation; phosphoramidite; public health relevance; success; three dimensional structure; ultra high resolution

DESCRIPTION (provided by applicant): The central dogma of molecular biology has undergone a significant revision where RNAs have emerged as important regulators of cell activity. Astonishingly, as much as 80% of human DNA is transcribed into RNA as recently reported by the Encyclopedia of DNA Elements (ENCODE) consortium. New classes of RNA are being identified including many which are non-protein coding (ncRNA). Nonetheless ncRNAs are involved in regulating cellular activity. Deciphering the structure-function relationship of these RNAs is of immediate importance as ncRNAs have now been implicated in a variety of diseases including myotonic dystrophy type 1, prostate cancer, spinal muscular atrophy, and Huntington's disease-like 2. Traditional approaches to determine high-resolution RNA 3D structures have proven to be prohibitively time consuming and expensive. Nymirum has developed an Automated RNA 3D Structure Determination System that is faster, cheaper, and more robust than current approaches. This System will be commercialized for use by the drug industry and academic researchers in their quest to understand and treat human disease.

描述（由申请人提供）：分子生物学的中心法则经历了重大修订，其中RNA已成为细胞活性的重要调节剂。令人惊讶的是，多达80%的人类DNA被转录成RNA，最近由DNA元件百科全书（ENCODE）财团报道。正在鉴定新的RNA类别，包括许多非蛋白质编码（ncRNA）。尽管如此，ncRNA参与调节细胞活性。破译这些RNA的结构-功能关系具有直接的重要性，因为ncRNA现在已经涉及多种疾病，包括强直性肌营养不良1型、前列腺癌、脊髓性肌萎缩和亨廷顿病样2。确定高分辨率RNA 3D结构的传统方法已被证明是非常耗时和昂贵的。Nymirum开发了一种自动RNA 3D结构测定系统，比目前的方法更快，更便宜，更强大。该系统将被商业化，供制药行业和学术研究人员用于了解和治疗人类疾病。