Ophthalmology Core Facility

 描述(由申请人提供)：这份P30眼科核心设施续签申请是为了在四个资源核心上为NEI资助的俄勒冈健康与科学大学(OHSU)的视觉研究人员提供支持：生物成像和共聚焦显微镜(Bio-Imaging)；基因表达和操纵(基因操纵)；分子遗传学和生物统计学(Genetics/Biostats)；以及蛋白质组学和质谱学(Proteology)。这些共享的资源将提供个人研究人员无法使用的设备和人员，这些设备和人员研究的疾病种类繁多，几乎影响到眼睛的所有部位，包括眼前段、小梁网、晶状体、视网膜和视神经。相关疾病包括青光眼、遗传性视网膜变性、黄斑变性、白内障、葡萄膜炎以及青光眼和黄斑变性的遗传学。生物成像将支持免疫荧光显微镜在组织中识别和定位蛋白质，并为使用Spectral DomainOCT的新模块提供支持，用于啮齿动物眼睛的活体成像。基因操作将为一系列方法提供技术支持和建议，以确定由于疾病状态或其治疗而导致的基因表达水平及其产物(蛋白质)的变化， 以及可以操纵这些反应的方法，如RNAi沉默、基因过度表达或通过转基因或病毒基因转移剂进行基因变异/突变表达。研究转录调控的方法也将可用。Genetics/Biostats将为NEI资助的研究人员提供从患者样本中分离DNA的服务，并将提供广泛的生物统计学支持，包括研究设计、数据分析方面的建议，以及通过高维数据、高级聚类分析和从微阵列数据预测转录因子等复杂方法的专家协助。蛋白质组学核心将提供以更高的灵敏度、吞吐量和准确性探测蛋白质结构的先进技术。所有四个核心具有很强的互补性，与部门视觉研究研讨会系列相结合，将营造积极的协作视觉研究氛围。

 DESCRIPTION (provided by applicant): This P30 Ophthalmology Core Facility renewal application is submitted to provide support for NEI-funded Oregon Health and Science University (OHSU) vision researchers in four Resource cores: Bio-Imaging & Confocal Microscopy (Bio-Imaging); Gene Expression & Manipulation (Gene Manipulation); Molecular Genetics & Biostatistics (Genetics/Biostats); and Proteomics & Mass Spectrometry (Proteomics). These shared resources will provide equipment and personnel that would otherwise not be available to individual researchers working in a diverse array of diseases affecting nearly all parts of the eye, including the anterior segment, trabecular meshwork, lens, retina, and optic nerve. Associated diseases include glaucoma, inherited retinal degenerations, macular degeneration, cataract, uveitis and the genetics of glaucoma and macular degeneration. Bio-Imaging will support immunofluorescence microscopy for identification and localization of proteins within tissues, as well as provide support for a new module using Spectral Domain OCT for in vivo imaging of rodent eyes. Gene manipulation will provide technical support and advice for a range of methods to identify changes in levels of gene expression and their products (proteins) that result either from disease states or their treatment, and for methods by which these responses can be manipulated, such as RNAi silencing, gene overexpression or gene variant/mutant expression via transfection or viral gene transfer agents. Methods for studying transcriptional regulation will also be available. Genetics/Biostats will provide DNA isolation services from patient samples to NEI-funded investigators, and will provide a wide range of biostatistical support, including advice on study design, data analysis, and expert assistance with sophisticated methods such as high dimensional data, advanced clustering analysis and transcription factor prediction from microarray data. The Proteomics core will provide access to advanced techniques for probing protein structure with higher sensitivity, throughput and accuracy. All 4 cores are highly complementary and, in combination with a departmental vision research seminar series, will foster a positive atmosphere of collaborative vision research.