Ophthalmology Core Facility

 描述（由申请人提供）：提交此P30眼科核心设施更新申请是为了在四个资源核心中为NEI资助的俄勒冈州健康与科学大学（OHSU）视觉研究人员提供支持：生物成像和共聚焦显微镜（生物成像）;基因表达和操作（基因操作）;分子遗传学和生物统计学（遗传学/生物统计学）;和蛋白质组学和质谱（蛋白质组学）。这些共享资源将提供设备和人员，否则将无法提供给个体研究人员，这些研究人员在影响眼睛几乎所有部位的各种疾病中工作，包括眼前节、小梁网、透镜、视网膜和视神经。相关疾病包括青光眼、遗传性视网膜变性、黄斑变性、白内障、葡萄膜炎以及青光眼和黄斑变性的遗传学。Bio-Imaging将支持免疫荧光显微镜用于组织内蛋白质的识别和定位，并为使用光谱域OCT进行啮齿动物眼睛体内成像的新模块提供支持。基因操作将为一系列方法提供技术支持和建议，以确定由疾病状态或治疗引起的基因表达水平及其产物（蛋白质）的变化， 以及用于操纵这些应答的方法，例如RNAi沉默、基因过表达或通过转染或病毒基因转移剂的基因变体/突变体表达。研究转录调控的方法也将是可用的。Genetics/Biostats将为NEI资助的研究者提供患者样本的DNA分离服务，并将提供广泛的生物统计支持，包括研究设计，数据分析和专家协助等复杂方法，如高维数据，先进的聚类分析和微阵列数据的转录因子预测。蛋白质组学核心将提供先进的技术，以更高的灵敏度，通量和准确性探测蛋白质结构。所有4个核心是高度互补的，并结合部门视觉研究研讨会系列，将促进合作视觉研究的积极氛围。

 DESCRIPTION (provided by applicant): This P30 Ophthalmology Core Facility renewal application is submitted to provide support for NEI-funded Oregon Health and Science University (OHSU) vision researchers in four Resource cores: Bio-Imaging & Confocal Microscopy (Bio-Imaging); Gene Expression & Manipulation (Gene Manipulation); Molecular Genetics & Biostatistics (Genetics/Biostats); and Proteomics & Mass Spectrometry (Proteomics). These shared resources will provide equipment and personnel that would otherwise not be available to individual researchers working in a diverse array of diseases affecting nearly all parts of the eye, including the anterior segment, trabecular meshwork, lens, retina, and optic nerve. Associated diseases include glaucoma, inherited retinal degenerations, macular degeneration, cataract, uveitis and the genetics of glaucoma and macular degeneration. Bio-Imaging will support immunofluorescence microscopy for identification and localization of proteins within tissues, as well as provide support for a new module using Spectral Domain OCT for in vivo imaging of rodent eyes. Gene manipulation will provide technical support and advice for a range of methods to identify changes in levels of gene expression and their products (proteins) that result either from disease states or their treatment, and for methods by which these responses can be manipulated, such as RNAi silencing, gene overexpression or gene variant/mutant expression via transfection or viral gene transfer agents. Methods for studying transcriptional regulation will also be available. Genetics/Biostats will provide DNA isolation services from patient samples to NEI-funded investigators, and will provide a wide range of biostatistical support, including advice on study design, data analysis, and expert assistance with sophisticated methods such as high dimensional data, advanced clustering analysis and transcription factor prediction from microarray data. The Proteomics core will provide access to advanced techniques for probing protein structure with higher sensitivity, throughput and accuracy. All 4 cores are highly complementary and, in combination with a departmental vision research seminar series, will foster a positive atmosphere of collaborative vision research.