Specimen Resource Core

标本资源核心是所有耶鲁大学皮肤癌孢子(YSPORE)活动的基石。它解决了所有项目的广泛黑色素瘤患者样本需求，这些项目目前在耶鲁大学的共享设施无法满足。核心不仅仅是履行孢子指南所规定的标本资源核心的职责。除了收集、存储和分发各种标本和试剂外，该核心还执行质量保证测试和对标本进行广泛的分子分析。更具体地说，核心：a)为黑色素瘤的翻译和临床前研究收集大量标本，包括黑色素细胞病变、黑色素瘤肿瘤和细胞、正常皮肤、血清和循环淋巴细胞；b)确保高质量的控制和适当的长期存储、注释和及时分发给YSPORE研究人员；c)建立和维护由YSPORE项目产生的基本病理、临床、流行病学、后续信息和基础研究数据的中央数据库，该数据库与生物信息学/生物统计核心相结合；D)提供特殊的服务，如分析临床试验的样本、突变、染色质修饰和收集肿瘤浸润性淋巴细胞；e)维护和分发经验证的试剂(抗体、用于聚合酶链式反应的寡核苷酸、DNA、RNA、质粒、细胞提取物)，以供不同的YSPORE研究人员进行肿瘤的分子分析；f)与耶鲁大学的共享设施和其他机构的类似资源建立联系，包括其他孢子，并向耶鲁大学以外的协作研究人员发送样本；以及g)为更大的NCI目标提供样本，包括黑色素瘤的标本、外周血淋巴细胞和癌症基因组图谱(TCGA)的注释临床数据。CORE与耶鲁综合癌症中心的生物信息学、临床试验办公室、免疫学、组织服务关键技术和遗传学共享设施中的每个项目的研究人员进行广泛的互动。标本资源核心的服务以经济高效的方式提高了YSPORE研究人员转译研究的效率，并加快了将试验台上的发现应用于临床实践，以及将临床结果应用于基础研究。

The Specimen Resource Core is the cornerstone of all Yale SPORE in Skin Cancer (YSPORE) activities. It addresses the broad melanoma patient specimen needs of all the projects that are not met by current shared facilities at Yale. The core goes beyond fulfilling the required duties of a Specimen Resource Core delineated by the SPORE guidelines. In addition to collecting, storing and distributing a wide range of specimens and reagents, the core performs quality assurance testing and a wide range of molecular analyses of specimens. More specifically, the core: a) collects a large repertoire of specimens for translational and preclinical studies in melanoma, including melanocytic lesions, melanoma tumors and cells, normal skin, serum, and circulating lymphocytes; b) ensures high quality control and proper long-term storage, annotation, and timely distribution of specimens to YSPORE investigators; c) establishes and maintains a central database of essential pathological, clinical, epidemiological, follow-up information and basic research data generated by the YSPORE projects that is integrated with the Bioinformatics/Biostatistics Core; d) provides special, services such as the analysis of specimens from clinical trials, mutations, chromatin modification, and collection of TlLs (tumor infiltrating lymphocytes; e) maintains and distributes validated reagents (antibodies, oligonucleotides for PCR, DNA, RNA, plasmids, cell extracts) needed for molecular analyses of tumors by different YSPORE investigators; f) establishes links with shared facilities at Yale and similar resources in other institutions, including other SPOREs and sends out samples to collaborating investigators outside of Yale; and g) provides specimens for larger NCI objectives, including melanoma specimens, peripheral blood lymphocytes, and annotated clinical data for The Cancer Genome Atlas (TCGA). The core Interacts extensively with investigators in each project, the Bioinformatics, Clinical Trial Office, Immunology, Critical Technologies for Tissue Services, and Genetics shared facilities of the Yale Comprehensive Cancer Center. The services of the Specimen Resource Core enhance the efficient operation of the translational studies by YSPORE investigators in a cost-effective manner, and expedite the application of discoveries at the bench to clinical practice, and clinical results to basic research.