Mechanism and clinical pilot tst of volar fibroblast to create ectopic volar skin

描述(由申请人提供)：拟议研究的长期目标是学习如何在再生医学中有效地使用细胞疗法，特别是与皮肤病相关的。特别是，我们的目标是将截肢者残端皮肤的身份转换为手掌/足掌(掌侧)型厚皮，以加强他们对假体的使用。为了达到这一临床目标，我们将首先定义掌侧成纤维细胞皮肤特性的基本遗传控制，然后我们将定义非掌侧组织保留其特性的动态平衡机制。在这样做的过程中，我们将优化使用自体成纤维细胞诱导志愿者异位掌侧皮肤的最后努力的条件。我们将完全切除这个异位肢端皮肤作为受孕证据试验，并作为临床优化和截肢者使用的前奏。

DESCRIPTION (provided by applicant): The long term objective of the proposed research is to learn how to effectively use cellular therapy in regenerative medicine, especially as pertains to dermatology. In particular, our goal is to convert the identity of skin at the stump site of amputees to thick palmo/plantar (volar) type skin to enhance their use of prosthetics. Towards this clinical goal we first will define the basic genetic control of volar fibroblast skin identity Then we will define homeostatic mechanisms whereby non-volar tissue retains its identity. In so doing, we will optimize conditions for our final effort to use autologous fibroblasts to induce ectopic volar skin in volunteers. We will fully excise this ectopic acral skin as a proof-of-concep trial and a prelude to clinical optimization and use in amputees.