Mechanism and clinical pilot tst of volar fibroblast to create ectopic volar skin

描述（由申请人提供）：拟议研究的长期目标是学习如何有效地在再生医学中使用细胞疗法，特别是与皮肤病学有关的细胞疗法。特别是，我们的目标是将截肢者残肢部位的皮肤转换为厚的掌/跖（掌）型皮肤，以提高他们对假肢的使用。为了实现这一临床目标，我们首先将确定掌侧成纤维细胞皮肤身份的基本遗传控制，然后我们将确定非掌侧组织保持其身份的自我平衡机制。在这样做的过程中，我们将优化条件，我们的最后努力，使用自体成纤维细胞诱导异位掌侧皮肤的志愿者。我们将完全切除这种异位肢端皮肤作为概念验证试验，并作为临床优化和在截肢者中使用的前奏。

DESCRIPTION (provided by applicant): The long term objective of the proposed research is to learn how to effectively use cellular therapy in regenerative medicine, especially as pertains to dermatology. In particular, our goal is to convert the identity of skin at the stump site of amputees to thick palmo/plantar (volar) type skin to enhance their use of prosthetics. Towards this clinical goal we first will define the basic genetic control of volar fibroblast skin identity Then we will define homeostatic mechanisms whereby non-volar tissue retains its identity. In so doing, we will optimize conditions for our final effort to use autologous fibroblasts to induce ectopic volar skin in volunteers. We will fully excise this ectopic acral skin as a proof-of-concep trial and a prelude to clinical optimization and use in amputees.