Injection of autologous volar fibroblasts to the stump site to allow pressure adaptation and enhanced prosthetic use in amputees

项目摘要 这项研究的长期目标是利用细胞疗法来推进再生医学。 我们特别希望帮助失去一条肢体的截肢者。虽然已经取得了重大改进， 在假肢中，残肢部位界面的皮肤侧没有进化成承受重量。树桩不 通常具有例如脚底的压力适应能力。本基金建议 活组织检查，纯化和扩大成纤维细胞从现有的手掌或鞋底，并将它们注射到残肢部位， 改善他们使用假肢的情况。我们还建议研究细胞治疗背后的基础生物学， 了解注入的细胞如何改变天然细胞，以及这些天然细胞如何影响注入的细胞。 细胞这项拨款的结果将有望导致一种新的治疗方法，不仅可以帮助截肢者， 预防压疮，并为未来的细胞疗法提供平台。

Project Summary Abstract The long term objective of the proposed research is to use cellular therapy to advance regenerative medicine. In particular, we wish to help amputees who have lost a limb. While major improvements have been made to prosthetics, the skin side of the interface at the stump site was not evolved to bear weight. The stump does not normally have the pressure adaptive abilities of the soles of the foot for example. The present grant proposes to biopsy, purify and expand fibroblasts from an existing palm or sole and inject them into the stump site of an amputee to improve their use of prosthetics. We also propose to study the basic biology behind cell therapy to understand how the injected cells change the native cells, and in turn how those native cells affect the injected cells. The results of this grant will hopefully lead to a new therapy to help not only amputees, but potentially prevent pressure ulcers and provide a platform for future cell therapies.