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Epigenetics, environmental exposure, and reproduction in the Collaborative Cross

协同杂交中的表观遗传学、环境暴露和繁殖

基本信息

批准号：
9063160
负责人：
David Lawrence Aylor
金额：
$ 23.59万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-17 至 2018-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9063160
关键词：
Adverse effects Alleles Analysis of Variance Animal Model Biological Biological Assay Biological Models Chemical Exposure Chromosome Mapping Complex DNA Methylation Data Diethylstilbestrol Endocrine Disruptors Environment Environmental Exposure Epigenetic Process Equilibrium Estrogens Exposure to Gene Expression Genes Genetic Genetic Models Genetic Variation Genome Goals Health Human Inbred Mouse Inbred Strain Inbred Strains Mice Inbreeding Individual Knowledge Link Male Genital Organs Maps Measures Mentors Methods Modeling Molecular Molecular Biology Molecular Weight Mus Neonatal Pathway interactions Phenotype Physiological Physiology Play Population Population Control Population Heterogeneity Postdoctoral Fellow Predisposition Property Protocols documentation Quantitative Trait Loci Randomized Reproducibility Reproduction Reproductive Physiology Reproductive system Research Resources Role Series Sperm Count Procedure System Testing Testis Tissues Translating Variant bisulfite sequencing causal model consumer product epigenetic regulation gene environment interaction genetic approach insight mRNA Expression male reproductive response trait xenoestrogen

项目摘要

Title: Epigenetics, environmental exposure, and reproduction in the Collaborative Cross PI/PD: David L. Aylor The proposed research is a systems genetics approach to characterize the complex relationships between genetic variation, epigenetic regulation, gene expression, male reproductive physiology, and environmental exposure. To accomplish this goal, we will leverage the power of diverse inbred mouse strains and the Collaborative Cross, a mouse reference population that has ideal properties for systems genetics. To identify gene-by-environment (G�E) interactions, we will perturb the reproductive system with the powerful estrogen diethylstilbestrol (DES) and phenotype identical exposed and control populations. We will measure DNA methylation, mRNA expression, and reproductive traits with the purpose of modeling genetic effects and G�E across three levels of genome function. This research will produce new biological knowledge of the genetic networks underlying the reproductive system, DES function at the molecular level, and genes underlying DES sensitivity.

表观遗传学，环境暴露和生殖在合作横 PI/PD：大卫L.艾勒拟议的研究是一种系统遗传学方法来表征该复合体遗传变异，表观遗传调控，基因表达，雄性生殖生理学和环境暴露。为了实现这一目标，我们将利用不同的近交系小鼠品系和协作杂交的力量，具有系统遗传学理想特性的小鼠参考群体。以识别基因与环境（GE）的相互作用，我们将干扰生殖系统，强大的雌激素己烯雌酚（DES）和表型相同的暴露，控制人口。我们将测量DNA甲基化，mRNA表达，生殖性状，目的是在三个群体中建模遗传效应和遗传效率。基因组功能水平。这项研究将产生新的生物学知识，作为生殖系统的遗传网络，DES在分子水平发挥作用，水平和DES敏感性的基因。