Epigenetics, environmental exposure, and reproduction in the Collaborative Cross

标题：合作中的表观遗传学、环境暴露和繁殖 十字 PI/PD：David L.Aylor 拟议的研究是一种系统遗传学方法来表征这种复合体。 遗传变异、表观遗传调控、基因表达与男性的关系 生殖生理学和环境暴露。为了实现这一目标，我们将 利用各种近交系小鼠品系和合作杂交的力量， 具有系统遗传学理想特性的小鼠参考种群。要确定 基因与环境(G�E)的相互作用，我们将通过以下方式扰乱生殖系统 强雌激素己烯雌酚(DES)和表型相同暴露和 控制人口数量。我们将测量DNA甲基化，mRNA表达，以及 繁殖性状，目的是模拟遗传效应和G�E 基因组功能的水平。这项研究将产生新的生物学知识 遗传网络是生殖系统的基础，DES在分子上发挥作用 水平，以及DES敏感性的基因。

Title: Epigenetics, environmental exposure, and reproduction in the Collaborative Cross PI/PD: David L. Aylor The proposed research is a systems genetics approach to characterize the complex relationships between genetic variation, epigenetic regulation, gene expression, male reproductive physiology, and environmental exposure. To accomplish this goal, we will leverage the power of diverse inbred mouse strains and the Collaborative Cross, a mouse reference population that has ideal properties for systems genetics. To identify gene-by-environment (G�E) interactions, we will perturb the reproductive system with the powerful estrogen diethylstilbestrol (DES) and phenotype identical exposed and control populations. We will measure DNA methylation, mRNA expression, and reproductive traits with the purpose of modeling genetic effects and G�E across three levels of genome function. This research will produce new biological knowledge of the genetic networks underlying the reproductive system, DES function at the molecular level, and genes underlying DES sensitivity.