An Accessible Optical Toolbox for Saturated Nanoscale Analysis of Neural Architecture
An Accessible Optical Toolbox for Saturated Nanoscale Analysis of Neural Architecture
批准号:
9169805
负责人:
Edward S. Boyden
金额:
$79.03万
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-07-01 至 2020-04-30
关键词:
3-DimensionalAddressAnatomyAnimalsAntibodiesAntigensArchitectureBindingBiological PreservationBiological ProcessBrainCell ShapeCellsChemicalsCodeColorCommunitiesCoupledDataElectron MicroscopyExtracellular SpaceFluorescence MicroscopyFluorescent in Situ HybridizationFormaldehydeGenerationsGeneticGenomeHippocampus (Brain)ImageIn SituIonsKnock-in MouseLabelLifeLightManualsMapsMembraneMethodologyMethodsMicroscopeMicroscopyMolecularMusNeuroanatomyNeurogliaNeuronsNeurosciencesOligonucleotidesOpticsPatternProbabilityProceduresProteinsProtocols documentationReporterResolutionSamplingShapesStaining methodStainsSucroseSynapsesTechnologyTestingThickThree-Dimensional ImageTimeTissue FixationTissuesTouch sensationTransgenic MiceViralanalogbrain cellbrain tissuebrain volumecell fixingcell typecomputer infrastructurecomputerized toolsdesigndigitalfluorophoreimaging modalityimaging probeimprovedlight microscopymicroscopic imagingnanoscalenervous system disorderneural circuitneuronal circuitrynovelnovel strategiesoptical imagingreconstructionrelating to nervous systemsample fixationtool
中文摘要
神经元完整结构的纳米级分辨率重建提供了
英文摘要
Nanoscale-resolution reconstructions of the complete architectures of neurons offer the
potential to revolutionize the study of neuronal circuitry in normal and diseased brains
but this promise is far from realized because the larger neuroscience community cannot
access the technologies that provide nanoscale reconstructions over large volumes and
the computational infrastructure required to analyze them. As a result, neuroanatomy at
the nanoscale is restricted to one-off studies of smaller volumes, rarely extending past
small volume reconstructions in single animals (n<=1), and accessible only to labs that
can afford the extraordinary time commitment, labor, and expense required to
reconstruct even simple neuronal circuits. Here we propose a novel approach: rather
than developing more and more sophisticated hardware for nanoscale reconstructions of
the brain, we propose to develop a toolbox of accessible molecular, chemical, and
computational approaches for accurate nanoscale reconstructions of large volumes of
the brain targeting fluorescence microscopy and the conventional confocal microscope –
two of the most widely accessed tools in the neuroscience arsenal. By providing an
accessible pipeline to reconstructing the natural shape and space between brain cells
found in the living brain over wide range of experimental conditions, we will help deliver
access to comprehensive nanoscale neuroanatomy to the broader neuroscience
community.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Mechanisms of pathology and neuronal hyperactivity in a memory circuit in Alzheimer's disease
-
批准号:10487389
-
项目类别:
-
资助金额:$64.42万
-
财政年份:2021
-
负责人:Edward S. Boyden
-
依托单位:
Mechanisms of pathology and neuronal hyperactivity in a memory circuit in Alzheimer's disease
-
批准号:10663344
-
项目类别:
-
资助金额:$64.42万
-
财政年份:2021
-
负责人:Edward S. Boyden
-
依托单位:
Multiplexed Nanoscale Protein Mapping Through Expansion Microscopy and Immuno-SABER
-
批准号:10088537
-
项目类别:
-
资助金额:$269.07万
-
财政年份:2020
-
负责人:Edward S. Boyden
-
依托单位:
High-throughput approaches to local and long-range synaptic connectivity
-
批准号:10025780
-
项目类别:
-
资助金额:$332.57万
-
财政年份:2020
-
负责人:Edward S. Boyden
-
依托单位:
RNA Scaffolds for Cell Specific Multiplexed Neural Observation
-
批准号:9981014
-
项目类别:
-
资助金额:$66.85万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
High-Performance Imaging Through Scattering Living Tissue
-
批准号:9369530
-
项目类别:
-
资助金额:$91.91万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
High-Performance Imaging Through Scattering Living Tissue
-
批准号:9978808
-
项目类别:
-
资助金额:$83.96万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
Scalable Cell- and Circuit-Targeted Electrophysiology
-
批准号:9893932
-
项目类别:
-
资助金额:$56.63万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
Expansion Microscopy
-
批准号:10609512
-
项目类别:
-
资助金额:$60.53万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
Expansion Microscopy
-
批准号:10442790
-
项目类别:
-
资助金额:$60.53万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
Expansion Microscopy
-
批准号:9301863
-
项目类别:
-
资助金额:$57.4万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
Expansion Microscopy
-
批准号:9925831
-
项目类别:
-
资助金额:$53.59万
-
财政年份:2017
-
负责人:Edward S. Boyden
-
依托单位:
High Speed, Multi-sensor Light Field Deconvolution Microscopy for Whole Brain Recording of Neuronal Activity
-
批准号:9222798
-
项目类别:
-
资助金额:$44.23万
-
财政年份:2016
-
负责人:Edward S. Boyden
-
依托单位:
Recording neural activities onto DNA
-
批准号:8743304
-
项目类别:
-
资助金额:$187.0万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
Millisecond-Timescale Whole-Brain Neural Activity Mapping in Health and Disease
-
批准号:8738739
-
项目类别:
-
资助金额:$77.22万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
Millisecond-Timescale Whole-Brain Neural Activity Mapping in Health and Disease
-
批准号:9119880
-
项目类别:
-
资助金额:$78.0万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
Millisecond-Timescale Whole-Brain Neural Activity Mapping in Health and Disease
-
批准号:8897460
-
项目类别:
-
资助金额:$78.0万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
Recording neural activities onto DNA
-
批准号:8547995
-
项目类别:
-
资助金额:$191.77万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
Recording neural activities onto DNA
-
批准号:8911380
-
项目类别:
-
资助金额:$187.0万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
Millisecond-Timescale Whole-Brain Neural Activity Mapping in Health and Disease
-
批准号:8564160
-
项目类别:
-
资助金额:$78.0万
-
财政年份:2013
-
负责人:Edward S. Boyden
-
依托单位:
海外基金