Acute Exercise Effects on Word Learning in Aging and Stroke-induced Aphasia

急性运动对衰老和中风引起的失语症的单词学习的影响

• 批准号: 9242454
• 负责人: Amy D Rodriguez
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-10-01 至 2018-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9242454
• 关键词: Acute; Address; Adult; Aerobic Exercise; Age; Age-Years; Age-associated memory impairment; Aging; Analysis of Covariance; Analysis of Variance; Animals; Anticoagulants; Aphasia; Behavioral; Blood; Blood specimen; Brain; Brain-Derived Neurotrophic Factor; Cognitive; Crossover Design; Disease; Dopamine; Elderly; Enzyme-Linked Immunosorbent Assay; Exercise; Feedback; Future; Goals; Hippocampus (Brain); Human; Immediate Recalls; Individual; Intervention; Investigation; Language; Learning; Levodopa; Literature; Lower Extremity; Measures; Mediating; Mediator of activation protein; Memory; Methods; Modeling; Names; Outcome; Performance; Personal Satisfaction; Physical Exercise; Physical assessment; Plasma; Population; Quality of life; Recruitment Activity; Research; Rest; Retrieval; Risk; Sampling; Serum; Short-Term Memory; Stretching; Stroke; Testing; Time; Training; Translational Research; Tube; Upper Extremity; Verbal Learning; Veterans; Whole Blood; base; disability; emerging adult; exercise intensity; healthy aging; improved; language impairment; long term memory; male; neurophysiology; neuropsychological; neuroregulation; novel; programs; psychologic; psychosocial; word learning; young adult

Objectives: Difficulty retrieving the right word to communicate a message is the most common complaint in healthy older adults and individuals with stroke-induced language impairment (i.e., aphasia). Novel word learning paradigms have been used to investigate a variety of behavioral and neuromodulatory approaches to improve word retrieval deficits. Previous novel word learning studies have shown that physical exercise can improve long term recall in healthy young adults and that increased levels of dopamine may be responsible for this effect; however, no studies have investigated the effects of exercise on novel word learning in older adults and individuals with aphasia, and very little is known about the mechanisms that support exercise-enhanced word learning in these populations. The aims of this SPiRE are to: 1) to identify the novel word learning paradigm that facilitates immediate and long-term word recall; 2a) to investigate how acute, moderate-intensity exercise alters levels of serum brain-derived neurotrophic factor (BDNF) and plasma dopamine; and 2b) to describe and quantify the association between novel word learning and any observed changes in serum BDNF and plasma dopamine. This research will provide a platform for future investigations of immediate exercise effects on word learning, which are essential for achieving our long-term goal of developing exercise-based word retrieval interventions for healthy older veterans and veterans with aphasia. Methods: A within subjects crossover design will be used to address Aims 1, 2a and 2b. Subjects will complete aerobic exercise or stretching (training) before engaging in Study Only or Retrieval Practice (learning). Subjects will be counterbalanced within and across conditions. Twelve healthy older veterans and 12 veterans with aphasia, age 65-89 years, will be recruited. The study will comprise 18 sessions over 12 weeks. In Week 1, subjects will undergo baseline blood draws, a physical assessment, cognitive and language assessments, and a practice word learning task. In Weeks 2-12, subjects will complete the four conditions. Each condition will last two weeks, with a one-week break between conditions. In each condition, training and learning sessions will take place on Monday, Wednesday and Friday. Training: Subjects will engage in 30 minutes of moderate-intensity cycling or gentle upper and lower limb stretching (control) prior to engaging in a novel word learning task. Learning: Familiar objects (e.g., cup) will be paired with nonwords (e.g., flark). In the Study Only paradigm, object/nonword pairs will be presented three times across three days (Monday, Wednesday, Friday). In the Retrieval Practice paradigm, object/nonword pairs will be presented during the first session (Monday). In subsequent sessions (Wednesday, Friday), subjects will be asked to recall the nonword name of presented objects. Corrective feedback will be provided on each trial. Testing: Recognition and recall testing without corrective feedback will be administered immediately after learning (short-term memory), before training in the second and third learning session (overnight consolidation) and one week after learning (long-term memory). Blood Draws: Three blood samples will be taken at baseline (rest, 30 minutes, 60 minutes). In each of the four conditions, blood samples will be taking during the first and third learning session (at rest, after training, and after learning). Whole blood will be collected in plain tubes and in anticoagulant-treated tubes for subsequent serum BDNF and plasma dopamine analysis by ELISA. Samples will be analyzed in triplicate to increase reliability. To address Aim 1, we will use a mixed effects model approach to fit repeated measures ANOVAs to word recall scores at after learning (immediate recall) and one week later (long-term recall). To address Aim 2a, we will use a mixed effects model approach to fit repeated measures ANOVA to serum BDNF and plasma dopamine levels obtained at rest, after 30 minutes and 60 minutes at baseline and during training sessions on Monday and Friday. To address Aim2b, we will use a mixed effects model approach to fit ANCOVA mediator analyses to investigate the association between neurophysiological changes (BDNF, dopamine) on word recall (immediate, long-term).

目标： 难以检索正确的单词来传达信息是最常见的抱怨 健康老年人和患有中风引起的语言障碍（即失语症）的人。小说词 学习范式已被用来研究各种行为和神经调节方法 改善单词检索缺陷。之前的新词学习研究表明，体育锻炼可以 改善健康年轻人的长期记忆，多巴胺水平的增加可能是 这种效果；然而，没有研究调查运动对老年人新词学习的影响 和失语症患者，但对于支持运动增强的机制知之甚少 这些人群的单词学习。该 SPiRE 的目标是：1）识别新词学习 促进即时和长期单词回忆的范式； 2a) 调查如何急性、中等强度 运动改变血清脑源性神经营养因子（BDNF）和血浆多巴胺的水平；和 2b) 描述 并量化新词学习与血清 BDNF 和血浆中任何观察到的变化之间的关联 多巴胺。这项研究将为未来研究锻炼对单词的即时影响提供一个平台 学习，这对于实现我们开发基于练习的单词检索的长期目标至关重要 对健康的老年退伍军人和患有失语症的退伍军人进行干预。方法：A 受试者内交叉 设计将用于实现目标 1、2a 和 2b。受试者将完成有氧运动或伸展运动 （培训），然后再进行仅学习或检索练习（学习）。科目将被平衡 在条件之内和条件之间。 12 名健康的老年退伍军人和 12 名患有失语症的退伍军人，年龄 65-89 岁，将 被招募。该研究将包括 12 周内的 18 个课程。在第一周，受试者将接受基线 抽血、身体评估、认知和语言评估以及练习单词学习任务。 在第 2-12 周，受试者将完成四个条件。每种情况将持续两周，其中一周 条件之间的中断。在每种情况下，培训和学习课程都将在周一举行， 周三和周五。训练：受试者将进行 30 分钟的中等强度骑行或温和骑行 在进行新单词学习任务之前伸展上肢和下肢（控制）。学习情况：熟悉 物体（例如杯子）将与非单词（例如flark）配对。在仅研究范式中，对象/非单词对 将在三天内（周一、周三、周五）展示三次。在检索实践中 范式、宾语/非单词对将在第一节会议（周一）期间呈现。在随后的会议中 （周三、周五），受试者将被要求回忆所呈现物体的非单词名称。矫正 每次试验都会提供反馈。测试：没有纠正反馈的识别和回忆测试将 在学习后立即进行（短期记忆），在第二次和第三次训练之前 学习期间（过夜巩固）和学习后一周（长期记忆）。抽血： 将在基线（休息、30 分钟、60 分钟）采集三份血样。在这四种情况中的每一种情况下， 将在第一次和第三次学习期间（休息时、训练后和训练后）采集血样 学习）。全血将收集在普通管和抗凝处理的管中用于随后的血清 通过 ELISA 分析 BDNF 和血浆多巴胺。样品将进行三次分析以提高可靠性。到 针对目标 1，我们将使用混合效应模型方法将重复测量方差分析与单词回忆得分进行拟合 学习后（立即回忆）和一周后（长期回忆）。为了实现目标 2a，我们将使用混合效果 模型方法将重复测量方差分析与静息时获得的血清 BDNF 和血浆多巴胺水平进行拟合 周一和周五的基线和训练期间分别为 30 分钟和 60 分钟。为了解决 Aim2b，我们将 使用混合效应模型方法来拟合 ANCOVA 中介分析来研究之间的关联 单词回忆（即时、长期）的神经生理学变化（BDNF、多巴胺）。