Core-001: Histological Assessment Core

Core-001：组织学评估核心

• 批准号: 9087505
• 负责人: Ernestina Schipani
• 金额: $ 25.76万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9087505
• 关键词: Applications Grants; Area; Arts; Biology; Bromodeoxyuridine; Cartilage; Cells; Chemistry; Collaborations; Communities; Consultations; Core Facility; Data Analyses; Devices; Disease; Education; Ensure; Equipment; Evaluation; Experimental Designs; Frozen Sections; Future; Generations; Genes; Goals; Guidelines; Health; Hematoxylin and Eosin Staining Method; Histologic; Histological Techniques; Histology; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Individual; Label; Laboratories; Ligaments; Link; Measurement; Methacrylates; Methodology; Methods; Michigan; Molecular; Molecular Biology; Muscle; Musculoskeletal; Outcome; Paraffin; Pattern; Phenotype; Plasmids; Preparation; Price; Procedures; Process; Protocols documentation; Publications; Reaction; Reagent; Recommendation; Research; Research Personnel; Resources; Services; Staining method; Stains; TdT-Mediated dUTP Nick End Labeling Assay; Techniques; Technology; Tendon structure; Time; Tissues; Training; Trichrome stain method; Universities; base; bone; combinatorial; cost effective; design; experience; flexibility; functional outcomes; in vivo; investigator training; mRNA Expression; meetings; novel; protocol development; research study; sample fixation; technology development; technology training; web site

Michigan Integrative Musculoskeletal Health Core Center – Core B Project Summary Histology is an essential component of virtually all in vivo musculoskeletal studies. The centralization of histologic efforts allows for high quality, reasonably priced histology, and ensures, in collaboration with the other Resource Cores, an efficient, integrated, hierarchical, and cost-effective approach to the evaluation of tissue phenotypes. The Histological Assessment Core (Core B) will provide investigators with access to state-of-the-art technologies, training on the use of techniques and devices, and consultation on experimental design, protocol development, data analysis and interpretation. Core B will include capabilities and expertise in the histologic assessment of cartilage and bone, particularly using paraffin and plastic sections as well as analysis of muscles, tendons, and ligaments and with experimental procedures performed on frozen sections. The Core will also maintain a plasmid stock for the synthesis of riboprobes and provide guidance in the use of these reagents on tissue sections. These offerings were chosen based on the needs across all musculoskeletal tissues of the Research Community at the University of Michigan. The services and facilities of the Core will be available to investigators on a first-come, first-served basis, although the general guidelines will be flexible to accommodate high priority deadlines, such as meetings, publications and grant application deadlines. Four Aims are proposed: the Core will provide expertise and guidance on the design of experiments in Aim 1, and expertise and training on the generation of high quality histological sections and the use of state-of-art histologic techniques in Aim 2. Further, in Aim 3, the Core will provide guidance with interpretation of results and make recommendations for future studies. Lastly, the overall goal of Aim 4 is to enable the research community through ongoing technology development and education. Centralizing the histological assessments of musculoskeletal tissues in a Core facility will leverage the expertise in this area of the Core staff and ensure high quality, efficient use of resources and equipment, proper training, and intellectual engagement with experts to advance understanding of musculoskeletal biology, health, and disease.

密歇根综合肌肉骨骼健康核心中心-核心B 项目摘要 组织学是几乎所有体内肌肉骨骼研究的重要组成部分。的 组织学工作的集中化允许高质量、价格合理的组织学， 确保与其他资源核心合作，建立一个有效的、综合的、分层次的、 和成本效益的方法来评估组织表型。组织学 评估核心（核心B）将为研究者提供最新技术水平的访问权限 技术、技术和设备使用方面的培训， 设计、方案制定、数据分析和解释。核心B将包括 和软骨和骨组织学评估的专业知识，特别是使用石蜡， 塑料切片以及肌肉，肌腱和韧带的分析，并与实验 在冷冻切片上进行的程序。核心还将维持质粒储备， 核糖核酸探针的合成，并在组织切片上使用这些试剂提供指导。 这些产品的选择是基于所有肌肉骨骼组织的需求， 密歇根大学的研究社区。核心的服务和设施将 调查人员可按先到先得的原则获得，但一般准则 将采取灵活办法，以适应会议、出版物和赠款等高度优先事项的最后期限 申请截止日期。提出了四个目标：核心小组将提供以下方面的专门知识和指导： 目标1中的实验设计，以及关于高质量生成的专业知识和培训 组织学切片和Aim 2中最先进的组织学技术的使用。此外，在目标3中， 核心小组将提供指导，解释结果，并提出建议， 未来的研究。最后，目标4的总体目标是使研究界能够通过 持续的技术开发和教育。集中组织学评估 核心设施中的肌肉骨骼组织将利用核心工作人员在该领域的专业知识 并确保高质量，有效利用资源和设备，适当的培训， 与专家进行智力交流，以增进对肌肉骨骼生物学的了解， 健康和疾病。