Core-001: Histological Assessment Core

密歇根州综合肌肉骨骼健康核心中心-核心B 项目摘要 组织学是几乎所有活体肌肉骨骼研究的重要组成部分。这个 组织学工作的集中化允许高质量、合理价格的组织学检查，以及 与其他资源核心协作，确保高效、集成、分层 和经济高效的方法来评估组织表型。组织学 评估核心(核心B)将为调查人员提供访问最先进技术的途径 技术，关于使用技术和装置的培训，以及关于实验的咨询 设计、协议开发、数据分析和解释。核心B将包括功能 在软骨和骨骼的组织学评估方面的专业知识，特别是使用石蜡和 塑料切片以及肌肉、肌腱和韧带的分析，并与实验 在冰冻切片上执行的程序。核心还将维护一个质粒库 核探针的合成，并为在组织切片上使用这些试剂提供指导。 这些产品是根据所有肌肉骨骼组织的需求选择的 密歇根大学的研究社区。核心的服务和设施将 以先到先得的方式向调查人员提供，尽管一般准则 将灵活适应高优先级的截止日期，如会议、出版物和赠款 申请截止日期。提出了四个目标：核心将在以下方面提供专门知识和指导 目标1中的实验设计，以及关于产生高质量产品的专门知识和培训 目标2中的组织切片和最新组织学技术的使用。此外，在目标3中， 核心将提供指导，解释结果，并为以下方面提出建议 未来的研究。最后，目标4的总体目标是使研究界能够通过 正在进行的技术开发和教育。将组织学评估集中于 核心设施中的肌肉骨骼组织将利用核心员工在这一领域的专业知识 并确保高质量、高效地使用资源和设备、适当的培训和 与专家进行智力接触，以增进对肌肉骨骼生物学的理解 健康和疾病。

Michigan Integrative Musculoskeletal Health Core Center – Core B Project Summary Histology is an essential component of virtually all in vivo musculoskeletal studies. The centralization of histologic efforts allows for high quality, reasonably priced histology, and ensures, in collaboration with the other Resource Cores, an efficient, integrated, hierarchical, and cost-effective approach to the evaluation of tissue phenotypes. The Histological Assessment Core (Core B) will provide investigators with access to state-of-the-art technologies, training on the use of techniques and devices, and consultation on experimental design, protocol development, data analysis and interpretation. Core B will include capabilities and expertise in the histologic assessment of cartilage and bone, particularly using paraffin and plastic sections as well as analysis of muscles, tendons, and ligaments and with experimental procedures performed on frozen sections. The Core will also maintain a plasmid stock for the synthesis of riboprobes and provide guidance in the use of these reagents on tissue sections. These offerings were chosen based on the needs across all musculoskeletal tissues of the Research Community at the University of Michigan. The services and facilities of the Core will be available to investigators on a first-come, first-served basis, although the general guidelines will be flexible to accommodate high priority deadlines, such as meetings, publications and grant application deadlines. Four Aims are proposed: the Core will provide expertise and guidance on the design of experiments in Aim 1, and expertise and training on the generation of high quality histological sections and the use of state-of-art histologic techniques in Aim 2. Further, in Aim 3, the Core will provide guidance with interpretation of results and make recommendations for future studies. Lastly, the overall goal of Aim 4 is to enable the research community through ongoing technology development and education. Centralizing the histological assessments of musculoskeletal tissues in a Core facility will leverage the expertise in this area of the Core staff and ensure high quality, efficient use of resources and equipment, proper training, and intellectual engagement with experts to advance understanding of musculoskeletal biology, health, and disease.