TRD2: Functional Imaging

项目摘要 TRD 2 TRD项目2将开发新型光学相干断层扫描（OCT）技术，以解决未满足的 组织功能成像的需求。具体目标#1解决了对高速和高性能的需求， 用于临床前研究的高分辨率血管造影成像系统。一种新的高速光学结构 高分辨率光学相干显微镜（OCM）将被构造成比 现有OCM仪器。这些速度上的改进将被用来扩展高 分辨率血管造影和定量血流成像，以更广泛的成像领域，并提高 这些测量的时间分辨率。具体目标#2建立在最近的发展基础上， 长深度范围、高速OCT架构，以创建术中OCT摄像机平台。 通过偏振敏感OCT的矢量双折射成像将被纳入相机 系统，以允许跨手术区域的光学双折射的成像。虽然这个平台技术 可以在许多外科手术中应用，它将被专门验证为一种手段， 周围神经损伤的功能成像。最后，具体目标3涉及以下需要： 在慢性炎症性疾病和囊性肺疾病的小动物模型中研究肺功能的改进工具 纤维化（CF）。为了研究炎症驱动的淋巴重塑对肺功能的影响， 将开发用于对小鼠气管的集合管成像的导管和方法， 验证.使用类似的导管和一套新的成像方法，体内平台提供了 在CF的雪貂模型中测量气道表面液体性质和粘膜纤毛运输将 被证明。通过这些目标，TRD项目2将开发，验证和提供创新的新技术， OCT技术提供给我们的合作项目研究人员和更广泛的研究社区。

Project Summary TRD 2 TRD project 2 will develop novel optical coherence tomography (OCT) technologies to address unmet needs in the functional imaging of tissue. Specific Aim #1 addresses the need for high-speed and high- resolution angiographic imaging systems for preclinical research. A new high-speed optical architecture for high-resolution optical coherence microscopy (OCM) will be constructed that is 100 times faster than existing OCM instrumentation. These improvements in speed will be leveraged to expand high resolution angiography and quantitative flow imaging to wider imaging fields, and to improve the temporal resolution of these measurements. Specific Aim #2 builds upon the recent development of long depth range, high-speed OCT architectures to create an intraoperative OCT camera platform. Vectorial birefringence imaging through polarization-sensitive OCT will be incorporated into the camera system to allow imagining of optical birefringence across a surgical field. While this platform technology can have application in numerous surgical procedures, it will be specifically validated as a means for functional imaging of peripheral nerve injuries. Finally, specific aim #3 addresses the need for improved tools to study lung function in small animal models of chronic inflammatory disease and cystic fibrosis (CF). To study the consequences of inflammation-driven lymphatic remodeling on lung function, catheters and methods to image the collecting lymphatics of the mouse trachea will be developed and validated. Using similar catheters and a new set of imaging methods, an in vivo platform providing measurements of airway surface liquid properties and mucociliary transport in ferret models of CF will be demonstrated. Through these aims, TRD project 2 will develop, validate, and deliver innovative new OCT technologies to our collaborating project investigators and to the broader research community.