Mediator Lipidomics Core

摘要 中介-脂质组学核心基金的目标是支持NCE、LSUHSC和我们地区其他机构的项目中的脂质组学研究，以便研究人员能够成功地实现他们提出的目标。此外，核心将帮助它们获得大量的外部资金，这反过来又将在五年科布雷资助期之后通过按服务收费计划为该核心的未来运作提供财政支持。中介-脂质组学核心包含超高效液相色谱-串联质谱仪、气相色谱、薄层色谱，以及分离、纯化、鉴定、量化和储存脂质介体及其前体脂类所需的所有材料。质谱仪、气相色谱、脂类样品制备系统和其他附件设施为大新奥尔良地区和整个路易斯安那州的相当大比例的生物医学研究人员和临床科学家提供服务。优先考虑神经科学卓越中心(NCE)内的研究人员。为了有效地支持研究人员，尤其是那些正在向NIH支持的独立主要研究人员发展的研究人员，我们为我们的Mediator-Lipidome核心提出了三个具体目标：1：帮助研究人员使用最先进的超高效液相色谱与串联质谱仪和气相色谱相结合的技术，研究和分析对神经元和神经系统重要的脂质介质、脂肪酸组成、脂质类别和分子物种。2：就样品制备和设备的使用提供专家咨询和指导。3：协助新的首席调查员和现有的首席调查员努力获得NIH R01、P01、P50、U54、T32等拨款，以及路易斯安那州董事会奖。调解人-脂质组学核心将提供专家咨询，以促进个人投资机构为其方法制定假设和具体目标。它还将有助于现有赠款的可持续性，以及科布雷过渡中心的总体可持续性。

Abstract The goal of the Mediator-Lipidomics Core Facility is to support the lipidomics studies in projects at the NCE, at LSUHSC, and within other institutions in our region so that investigators are able to successfully fulfill their proposed goals. Furthermore, the core will help them to obtain substantial extramural funds, which in turn will financially support the future operation of this Core through a fee-for-service plan after the five-year COBRE-funded period. The Mediator-Lipidomics Core contains the ultra-performance liquid chromatography - tandem mass spectrometers, gas chromatography, thin layer chromatography, and all necessary materials for the isolation, purification, identification, quantification, and storage of lipid mediators and their precursor lipids. The mass spectrometry, gas chromatography, lipidomic sample preparation systems, and other accessories, facilities service a significant percentage of the biomedical researchers and clinical scientists in the greater New Orleans area and throughout the state of Louisiana. Priority is given to investigators within the Neuroscience Center of Excellence (NCE). To efficiently support the investigators, especially those who are developing their career towards NIH-supported independent principal investigators, we propose three Specific Aims for our Mediator-Lipidomics Core: 1: To assist investigators with state-of-the-art technologies of ultraperformance liquid chromatography coupled with tandem mass spectrometry and gas chromatography for studying and analyzing lipid mediators, fatty acid composition, lipid classes and molecular species important for neurons and the nervous system. 2: To provide expert advice and guidance on sample preparation and use of the equipment. 3: To provide assistance to new principal investigators (NPIs) and established Pis in their efforts to obtain NIH R01, P01, P50, U54, T32 grants, etc., as well as Louisiana Board of Regents Awards. The Mediator-Lipidomics Core will provide expert consultation to facilitate PIs to develop hypotheses and specific aims for their approaches. It will also contribute to the sustainability of existing grants, and overall, the COBRE Transitional Center.