COBRE: LSU: LIPIDOMIC CORE RESOURCE MODULE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该核心模块为研究脂质信号的实验设计提供指导和建议。该核心还协助和培训Cobre研究人员进行组织细胞采样、处理和提取，然后使用串联液相色谱-光电二极管阵列-电喷雾电离-串联MS/MS脂类分析进行脂类纯化和/或衍生，以确定腐烂的脂类信使的结构特征。高效液相色谱-光电二极管阵列-电喷雾电离-大气压化学电离-串联质谱仪是在最先进的三重四极LC-MS或离子陷阱上进行的，从而提供了强大而灵活的脂类介体的定量分析和结构表征。其他分离技术也是可用的，如高效液相、气相色谱、薄层色谱和固相萃取，以及通过放射、放射免疫分析或荧光应用进行分析。COBRE参与者得到数据处理和解释方面的技术支持和协助。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core module provides guidance and advice for experimental design to study lipid signaling. This core also assists and trains COBRE investigators on tissue cell sampling, processing, and extraction, followed by lipid purification and/or derivation for structural characterization of putattive lipid messengers using tandem liquid chromatography-photodiode array-electrospray ionization-tandem MS/MS-based lipidmoic analysis. Liquid chromatography-photodiode array-electrospray ionization-atmospheric pressure chemical ionization-tandem mass spectrometry is performed on state-of-the-art triple quadrupole LC-MS, or an ion trap, thus providing powerful and flexible quantitative analysis and structural characterization of lipid mediators. other separation techniques are also available, such as HPLC, GLC, TLC, and solid-phase extrction, as well as analysis by radiometric, radio-immunoassay, or fluorometric applications. COBRE participants receive technical support and assistance on data processing and interpretation.