Assembly and partition mechanism of Walker-box based segregation machinery

基于Walker-box的分离机械的组装和分离机构

基本信息

  • 批准号:
    9118256
  • 负责人:
  • 金额:
    $ 30.93万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-08-01 至 2019-04-30
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): DNA segregation or partition is one of the most fundamental processes in biology and ensures that genetic information is accurately retained from one generation to the next. While the molecules involved in partition have largely been identified in prokaryotes and eukaryotes, the mechanisms that drive this process are not understood at the atomic level. Moreover, currently, very little is known about DNA segregation in archaea. The overarching goals of this proposal are to define the molecular mechanisms and principles involved in DNA segregation using model bacterial and archaeal partition (par) systems. Bacterial plasmid par systems represent excellent model systems to study segregation at an atomic level because they minimally require only 3 elements, a centromere, a centromere-binding protein (CBP) and an NTPase. The most common par system is the so-called type I, or Walker-box based system. This type of par system is involved in the partitioning of both prokaryotic chromosomes and plasmids, and includes CBPs called ParB and Walker-type NTPases called ParA. Our recent combination of structural and in vivo super-resolution studies has started to provide a detailed atomic as well as dynamic understanding of bacterial segregation. Eukaryotes appear to use machinery distinct from prokaryotes whereby their centromeres are marked by "centromeric nucleosomes", which contain CenpA in the place of histone H3 along with H4, H2A and H2B. Although these systems seem vastly disparate, our recent studies have revealed structural and, potentially, functional links between DNA segregation in the 3 domains of life. Specifically, our initial characterization of the first archaa par system, pNOB8, reveals that it employs a Walker-box NTPase similar to those used by prokaryotes while our preliminary structure of the pNOB8 ParB reveals that it contains a fold similar to the eukaryotic histone CenpA protein. Based on these findings, we hypothesize that shared structural and functional principles may exist in DNA segregation mechanisms across the domains of life. The goals of this proposal are to provide detailed structural and functional dissections of DNA segregation using two prototypical model systems; the bacterial TP228 par system and the archaeal pNOB8 par system.


项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Maria Schumacher其他文献

Maria Schumacher的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Maria Schumacher', 18)}}的其他基金

Deciphering fundamental biological processes involving protein-nucleic acid interactions at the molecular level
破译涉及分子水平上蛋白质-核酸相互作用的基本生物过程
  • 批准号:
    10622948
  • 财政年份:
    2019
  • 资助金额:
    $ 30.93万
  • 项目类别:
Deciphering fundamental biological processes involving protein-nucleic acid interactions at the molecular level
破译涉及分子水平上蛋白质-核酸相互作用的基本生物过程
  • 批准号:
    10543420
  • 财政年份:
    2019
  • 资助金额:
    $ 30.93万
  • 项目类别:
Deciphering fundamental biological processes involving protein-nucleic acid interactions at the molecular level
破译涉及分子水平上蛋白质-核酸相互作用的基本生物过程
  • 批准号:
    10319963
  • 财政年份:
    2019
  • 资助金额:
    $ 30.93万
  • 项目类别:
Assembly and partition mechanism of Walker-box based segregation machinery
基于Walker-box的分离机械的组装和分离机构
  • 批准号:
    8941756
  • 财政年份:
    2015
  • 资助金额:
    $ 30.93万
  • 项目类别:
Complete atomic dissection of the B. subtilis nitrogen regulatory pathway
枯草芽孢杆菌氮调节途径的完整原子解剖
  • 批准号:
    9313913
  • 财政年份:
    2015
  • 资助金额:
    $ 30.93万
  • 项目类别:
Complete atomic dissection of the B. subtilis nitrogen regulatory pathway
枯草芽孢杆菌氮调节途径的完整原子解剖
  • 批准号:
    9118245
  • 财政年份:
    2015
  • 资助金额:
    $ 30.93万
  • 项目类别:
Protein Design, Expression and Purification Core
蛋白质设计、表达和纯化核心
  • 批准号:
    8931201
  • 财政年份:
    2015
  • 资助金额:
    $ 30.93万
  • 项目类别:
Structural mechanism of DNA segregation by the pSK41 par system
pSK41 par系统DNA分离的结构机制
  • 批准号:
    8236042
  • 财政年份:
    2009
  • 资助金额:
    $ 30.93万
  • 项目类别:
SAXS STUDIES ON P1 PARTITION COMPLEXES
P1 划分复合体的 SAXS 研究
  • 批准号:
    7954359
  • 财政年份:
    2009
  • 资助金额:
    $ 30.93万
  • 项目类别:
Structural mechanism of DNA segregation by the pSK41 par system
pSK41 par系统DNA分离的结构机制
  • 批准号:
    7728001
  • 财政年份:
    2009
  • 资助金额:
    $ 30.93万
  • 项目类别:

相似海外基金

Harnessing the potential of Archaea - Training Europe's next visionaries for an innovative and sustainable future
利用古生菌的潜力 - 培训欧洲的下一代有远见的人以实现创新和可持续的未来
  • 批准号:
    EP/Y031407/1
  • 财政年份:
    2024
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Research Grant
The mobilome of the anaerobic methanotrophic archaea Methanoperedenaceae
厌氧甲烷氧化古菌 Methanoperedenaceae 的移动组
  • 批准号:
    DP240100992
  • 财政年份:
    2024
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Discovery Projects
Asgard archaea: the first eukaryotic cells?
阿斯加德古菌:第一个真核细胞?
  • 批准号:
    DP230100769
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Discovery Projects
Probing the mechanisms that couple genome segregation to chromosome organization in Archaea
探讨古细菌基因组分离与染色体组织的耦合机制
  • 批准号:
    BB/X00645X/1
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Research Grant
Probing the mechanisms that couple genome segregation to chromosome organisation in Archaea
探讨古细菌基因组分离与染色体组织的耦合机制
  • 批准号:
    BB/X006506/1
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Research Grant
Enigmatic archaea in subsurface environments
地下环境中神秘的古细菌
  • 批准号:
    23H00387
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Functional and structural analysis of proline dehydrogenase for elucidation of proline biosynthesis in hyperthermophilic archaea
脯氨酸脱氢酶的功能和结构分析,用于阐明超嗜热古菌中脯氨酸的生物合成
  • 批准号:
    23K05658
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of novel denitrification process by methanotrophic archaea in bioelectroche mical system
生物电化学系统中甲烷氧化古菌新型反硝化工艺的发展
  • 批准号:
    22KF0377
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Grant-in-Aid for JSPS Fellows
Conference: 2023 Archaea: Ecology, Metabolism and Molecular Biology GRC and GRS The Root and Branch of Discovery: Lessons on Life from the Archaea
会议:2023 古细菌:生态学、代谢和分子生物学 GRC 和 GRS 发现的根源和分支:古细菌的生命教训
  • 批准号:
    2324896
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Standard Grant
Elucidation of biological and metabolic diversity of methanogenic archaea and reconstruction of subsurface carbon cycle
阐明产甲烷古菌的生物和代谢多样性以及重建地下碳循环
  • 批准号:
    23H03534
  • 财政年份:
    2023
  • 资助金额:
    $ 30.93万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了