Physiological Characterization and Data Integration Core

项目总结/摘要 本计划项目中提出的纵向研究所需的管理是双重的。第一，复杂 然而，糖尿病胃轻瘫的全面适当的动物模型需要每天监测以达到 治疗干预和机制分子研究所需的状态。第二，建议 机制分子研究包括来自表观基因组学、转录组学和蛋白质组学的大量数据集 接近。生理表征和数据集成核心C将提供基础设施 需要支持纵向动物研究以及不同的和大的动物模型的整合和可视化。 数据集。第一个目标是，核心将开发和维护一个平台， 整合项目中收集的数据。核心C基础设施的中心是一个数据库，即电子 动物研究记录（EARR），专门为该计划项目设计，因为商业解决方案 不能满足整体需求。EARR管理生理实验的工作流程， 生理数据，并将所有数据集成到程序项目中。EARR为项目调查人员提供 能够可视化项目内部或项目之间的不同数据，并提供快速公开共享的机制。 EARR保持灵活性，并不断完善，以满足项目调查人员不断变化的需求。第二 该研究核心的目的是提供表征动物生理学和动物活检所需的服务。 计划项目中使用的人类受试者。葡萄糖的中枢功能与胃排空 核心试验为该计划中的所有动物模型提供了一个有效和标准化的护理模式 项目这提高了数据的吞吐量、容量和质量。核心C继续扩大专业知识， 生理表征，并在这方面支持项目3所需的资源和人员， 在十二指肠粘膜活检中检测跨上皮阻力、大分子通量和神经源性分泌。 总体而言，核心C将促进项目实现各自的研究目标，并促进数据 项目内部和项目之间的整合。所有项目都统一在识别分子的总体目标中， 糖尿病胃轻瘫的机制，这将导致临床试验，以测试新的治疗干预措施。的 生理表征和数据集成核心C将有助于确保实现这一目标。

Project Summary/Abstract Management required for the longitudinal studies proposed in this Program Project is two-fold. First, the complex yet comprehensively appropriate animal models of diabetic gastroparesis require daily monitoring to reach the status required for therapeutic intervention and mechanistic molecular studies. Second, the proposed mechanistic molecular studies include large data sets from epigenomic, transcriptomic and proteomic approaches. The Physiological Characterization and Data Integration Core C will provide the infrastructure required to support the longitudinal animal studies and the integration and visualization of disparate and large data sets. The first aim is that the Core will develop and maintain a platform that effectively and efficiently integrates data collected in the Program Project. Central to Core C infrastructure is a database, the Electronic Animal Research Record (EARR), designed specifically for this Program Project because commercial solutions do not meet the overall needs required. EARR manages the workflow of physiological experiments, stores physiological data and integrates all data within the Program Project. EARR provides Project investigators the ability to visualize disparate data within or between Projects, and provide mechanisms for rapid public sharing. EARR remains flexible and is constantly refined to meet the evolving needs of Project investigators. The second aim of this research core is to provide services required to characterize the physiology of animals and biopsies of human subjects used within the Program Project. The centralized function of glucose and gastric emptying testing within the Core provides an efficient and standardized model of care for all animal models in the Program Project. This increases the throughput, capacity and quality of the data. Core C continues to expand expertise in physiological characterization and in this regard supports Project 3 with the resources and personnel required to test the transepithelial resistance, macromolecular flux, and neurogenic secretion in duodenal mucosal biopsies. Collectively, Core C will facilitate the Projects to reach their respective research aims, and facilitate data integration within and between Projects. All Projects are united in the overall objective to identify molecular mechanisms of diabetic gastroparesis that will lead to clinical trials to test novel therapeutic interventions. The Physiological Characterization and Data Integration Core C will help to make sure this objective is reached.