Physiological Characterization and Data Integration Core

生理表征和数据集成核心

• 批准号: 9109604
• 负责人: David R. Linden
• 金额: $ 27.03万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9109604
• 关键词: Animal Experimentation; Animal Housing; Animal Husbandry; Animal Model; Animals; Area; Biological Assay; Biopsy; Blood Glucose; Breath Tests; Breeding; Carbon Dioxide; Caring; Clinic; Clinical Sciences; Clinical Trials; Communities; Complex; Computer Hardware; Computer software; Data; Data Collection; Data Quality; Data Set; Data Storage and Retrieval; Databases; Deposition; Development; Diabetes Mellitus; Diabetic mouse; Disease; Electronics; Enteral; Epithelial; Equipment; Food; Funding; Gastric Emptying; Glucose; Health; Human Resources; Imagery; Individual; Interstitial Cell of Cajal; Intestines; Lead; Location; Longitudinal Studies; Measurement; Measures; Modeling; Molecular; Monitor; Mus; Nitrergic Neurons; Octanoic Acids; Physiological; Physiology; Proteomics; Research; Research Infrastructure; Research Personnel; Resistance; Resources; Science; Services; Stomach; System; Testing; Therapeutic; Therapeutic Intervention; Translational Research; abstracting; animal care; animal data; base; data integration; data integrity; data management; data visualization; design; diabetic gastroparesis; epigenomics; flexibility; gastrointestinal symptom; genetic pedigree; human disease; human subject; human tissue; improved; in vivo; insight; longitudinal animal study; meetings; next generation sequencing; novel; novel strategies; novel therapeutic intervention; novel therapeutics; programs; research study; restoration; stomach motility; tool; transcriptome sequencing; transcriptomics

Project Summary/Abstract Management required for the longitudinal studies proposed in this Program Project is two-fold. First, the complex yet comprehensively appropriate animal models of diabetic gastroparesis require daily monitoring to reach the status required for therapeutic intervention and mechanistic molecular studies. Second, the proposed mechanistic molecular studies include large data sets from epigenomic, transcriptomic and proteomic approaches. The Physiological Characterization and Data Integration Core C will provide the infrastructure required to support the longitudinal animal studies and the integration and visualization of disparate and large data sets. The first aim is that the Core will develop and maintain a platform that effectively and efficiently integrates data collected in the Program Project. Central to Core C infrastructure is a database, the Electronic Animal Research Record (EARR), designed specifically for this Program Project because commercial solutions do not meet the overall needs required. EARR manages the workflow of physiological experiments, stores physiological data and integrates all data within the Program Project. EARR provides Project investigators the ability to visualize disparate data within or between Projects, and provide mechanisms for rapid public sharing. EARR remains flexible and is constantly refined to meet the evolving needs of Project investigators. The second aim of this research core is to provide services required to characterize the physiology of animals and biopsies of human subjects used within the Program Project. The centralized function of glucose and gastric emptying testing within the Core provides an efficient and standardized model of care for all animal models in the Program Project. This increases the throughput, capacity and quality of the data. Core C continues to expand expertise in physiological characterization and in this regard supports Project 3 with the resources and personnel required to test the transepithelial resistance, macromolecular flux, and neurogenic secretion in duodenal mucosal biopsies. Collectively, Core C will facilitate the Projects to reach their respective research aims, and facilitate data integration within and between Projects. All Projects are united in the overall objective to identify molecular mechanisms of diabetic gastroparesis that will lead to clinical trials to test novel therapeutic interventions. The Physiological Characterization and Data Integration Core C will help to make sure this objective is reached.

项目概要/摘要 本计划项目中提出的纵向研究所需的管理有两个方面。一、综合体 然而，全面合适的糖尿病胃轻瘫动物模型需要每日监测才能达到 治疗干预和机制分子研究所需的状态。二、建议 机制分子研究包括表观基因组、转录组和蛋白质组的大数据集 接近。生理表征和数据集成核心 C 将提供基础设施 需要支持纵向动物研究以及不同和大型的整合和可视化 数据集。第一个目标是核心将开发和维护一个有效且高效的平台 整合了计划项目中收集的数据。 Core C 基础设施的核心是数据库、电子 动物研究记录 (EARR)，专门为此计划项目设计，因为商业解决方案 不能满足总体需求。 EARR 管理生理实验、商店的工作流程 生理数据并整合项目项目中的所有数据。 EARR 为项目调查人员提供 能够可视化项目内部或项目之间的不同数据，并提供快速公共共享的机制。 EARR 保持灵活性并不断完善，以满足项目调查人员不断变化的需求。第二个 该研究核心的目的是提供表征动物生理学和活组织检查所需的服务 计划项目中使用的人类受试者。葡萄糖和胃排空的集中功能 核心内的测试为该计划中的所有动物模型提供了高效且标准化的护理模型 项目。这提高了数据的吞吐量、容量和质量。 Core C 继续扩大专业知识 生理特征，并在这方面支持项目 3，提供所需的资源和人员 测试十二指肠粘膜活检中的跨上皮阻力、大分子通量和神经源性分泌。 总的来说，核心 C 将促进项目实现各自的研究目标，并促进数据 项目内部和项目之间的集成。所有项目的总体目标都是统一的，以识别分子 糖尿病胃轻瘫的机制将导致临床试验来测试新的治疗干预措施。这 生理表征和数据集成 Core C 将有助于确保实现这一目标。