Physiological Characterization and Data Integration Core

项目摘要/摘要 本计划项目中提出的纵向研究所需的管理是双重的。第一，建筑群 然而，全面合适的糖尿病胃轻瘫动物模型需要每日监测才能达到 治疗干预和机械性分子研究所需的状态。第二，建议的 机械性分子研究包括来自表观基因组、转录和蛋白质组的大数据集 接近了。生理特征和数据集成核心C将提供基础设施 支持纵向动物研究以及不同的和大型的 数据集。第一个目标是核心将开发和维护一个有效和高效的平台 整合计划项目中收集的数据。核心C基础架构的核心是一个数据库，即电子 动物研究记录(EARR)，专门为该计划项目设计，因为商业解决方案 不能满足要求的整体需求。EARR管理生理实验的工作流程，存储 生理数据，并将所有数据整合到计划项目中。EARR为项目调查员提供 能够可视化项目内或项目之间的不同数据，并提供快速公开共享的机制。 EARR保持灵活性，并不断改进，以满足项目调查人员不断变化的需求。第二 这项研究的核心目的是提供所需的服务，以表征动物的生理学和活组织检查 计划项目中使用的人类受试者。葡萄糖的中枢作用与胃排空 核心内的测试为计划中的所有动物模型提供了高效和标准化的护理模式 项目。这提高了数据的吞吐量、容量和质量。Core C继续扩展在以下方面的专业知识 生理特性，并在这方面为项目3提供所需的资源和人员 检测十二指肠粘膜活检组织的跨上皮阻力、大分子流量和神经源性分泌。 总体而言，核心C将促进项目达到各自的研究目标，并促进数据 项目内部和项目之间的集成。所有项目都在总目标上统一起来，以确定分子 糖尿病胃轻瘫的机制将导致临床试验，以测试新的治疗干预措施。这个 生理特征和数据集成核心C将有助于确保实现这一目标。

Project Summary/Abstract Management required for the longitudinal studies proposed in this Program Project is two-fold. First, the complex yet comprehensively appropriate animal models of diabetic gastroparesis require daily monitoring to reach the status required for therapeutic intervention and mechanistic molecular studies. Second, the proposed mechanistic molecular studies include large data sets from epigenomic, transcriptomic and proteomic approaches. The Physiological Characterization and Data Integration Core C will provide the infrastructure required to support the longitudinal animal studies and the integration and visualization of disparate and large data sets. The first aim is that the Core will develop and maintain a platform that effectively and efficiently integrates data collected in the Program Project. Central to Core C infrastructure is a database, the Electronic Animal Research Record (EARR), designed specifically for this Program Project because commercial solutions do not meet the overall needs required. EARR manages the workflow of physiological experiments, stores physiological data and integrates all data within the Program Project. EARR provides Project investigators the ability to visualize disparate data within or between Projects, and provide mechanisms for rapid public sharing. EARR remains flexible and is constantly refined to meet the evolving needs of Project investigators. The second aim of this research core is to provide services required to characterize the physiology of animals and biopsies of human subjects used within the Program Project. The centralized function of glucose and gastric emptying testing within the Core provides an efficient and standardized model of care for all animal models in the Program Project. This increases the throughput, capacity and quality of the data. Core C continues to expand expertise in physiological characterization and in this regard supports Project 3 with the resources and personnel required to test the transepithelial resistance, macromolecular flux, and neurogenic secretion in duodenal mucosal biopsies. Collectively, Core C will facilitate the Projects to reach their respective research aims, and facilitate data integration within and between Projects. All Projects are united in the overall objective to identify molecular mechanisms of diabetic gastroparesis that will lead to clinical trials to test novel therapeutic interventions. The Physiological Characterization and Data Integration Core C will help to make sure this objective is reached.