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Phage Display to Identify Epithelial to Mesenchymal Transitioned (EMT) Breast Cancer Cells

噬菌体展示鉴定上皮间质转化 (EMT) 乳腺癌细胞

基本信息

批准号：
9072699
负责人：
Deepa Bedi
金额：
$ 14.7万
依托单位：
TUSKEGEE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-30 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9072699
关键词：
Affinity Bacteriophages Binding Biological Markers Blood Circulation Brain Breast Cancer Cell Breast Carcinoma Cells Characteristics Complex Development Diagnostic Disease Management Disseminated Malignant Neoplasm Distant Drug Delivery Systems Epithelial Epithelial Cells Event Fibroblasts Fibronectins Figs - dietary Gene Delivery Generations Goals Human Image Invaded Lead Libraries Ligand Binding Liver Lung Magnetic Resonance Imaging Malignant - descriptor Malignant Neoplasms Mammary Neoplasms Mesenchymal Metastatic breast cancer Modeling Morphology Neoplasm Circulating Cells Neoplasm Metastasis Optics Organ Outcome Study Peptide Library Peptides Phage Display Phenotype Play Population Preparation Primary Neoplasm Process Role Scheme Specificity Surface Technology Testing Therapeutic Therapeutic antibodies Tissues Tumor Cell Invasion Vaccines Vimentin base bone cancer cell cell motility cell transformation comparative epithelial to mesenchymal transition innovation malignant breast neoplasm molecular imaging nanodevice neoplastic cell novel novel strategies public health relevance receptor residence screening specific biomarkers trait tumor tumor microenvironment tumor progression

项目摘要

DESCRIPTION (provided by applicant): The main goal of this study is to identify probes/markers for epithelial to mesenchymal transformed (EMT) breast cancer cells. Epithelial to Mesenchymal transition of cancer cells is a crucial phenomenon demonstrated by the epithelial type cancer cells in order to invade, and metastasize to distant organs. It is a process of loss of epithelial characteristics and the acquisition of a mesenchymal-like phenotype of cancer cells. It is thought to precede and enable the dissemination of tumor cells into the surrounding tissue and circulation. However characterization of these transformed cells has been challenging because of the non-specificity of the mesenchymal markers used to define them. So the picture of EMT and subsequent invasion is relatively unclear and warrants a more ambitious set of specific markers that can identify these transformed cell population and distinguish them from other mesenchymal cells such as fibroblasts. A new challenge, within this frame of concept, is to find specific set of biomarkers relevant for this process. We propose a novel and innovative study to use phage display libraries for identification of phages that can specifically and selectively bind to the EMT transitioned breast cancer cells invitro, in human breasts cancer tissues and help to distinguish them from the surrounding fibroblasts. This whole study is directed to obtain phage ligands binding to EMT-specific breast cancer cells. The project will include: 1) Selection and characterization phages that bind with high affinity, selectivity and specificity to the panel of human metastatic breast cancer cells after subtractive screening against mammary carcinoma- associated fibroblasts (CAF's). 2) Testing the binding of the selected phages to EMT-induced models of breast cancer cells and 3) Testing the binding of the selected phages to human breast cancer tissue (exvivio). Successful accomplishment of this project would lead to the development of the novel generation of phage-derived probes that can be further used for diagnostics, therapy, profiling and imaging.