Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure

描述（由申请人提供）：重新提交一项新的由PRACTIGATOR领导的纵向研究是对以下问题的回应：（PA-11-104）并将审查：（1）影响（GXE）的遗传和心理环境因素（歧视，抑郁，和养育行为）对3 - 5岁非裔美国人（AA）儿童健康的影响;（2）GXE高血压（HTN）风险在戒酒协会的妇女和儿童身上这个项目的总体目标是描绘的心理生物学（GXE互动）机制，通过AA母亲的感知种族歧视，心理健康和养育行为影响自己和他们的幼儿的血压（BP）随着时间的推移。我们的研究将研究遗传（候选基因和表观遗传效应）和心理（母亲感知的种族歧视，心理健康和养育行为）对BP的相互作用对这一人群的影响。我们将采用心理生物学方法：利用心理和生物学评估，采用纵向队列研究设计，2步候选基因和表观遗传方法，并使用祖先信息标记来解释人群分层混合，以探索AA母亲和儿童的BP GXE相互作用。将使用多重混合建模、交叉验证和错误发现率方法进行研究的数据分析。我们将招募250名3至5岁的AA儿童及其（n=250）母亲/母亲照顾者。我们将每六个月评估一次母亲和孩子的因素，为期两年。我们建议如下：目标1：检查GXE的相互作用和母亲的种族歧视的感知和其对BP的影响，在一段时间内的表观遗传效应超过两年。目标二：检查GXE相互作用和母亲的心理健康状况[抑郁症状]的表观遗传效应及其对BP的影响，为期两年。目的3：研究母亲养育行为的GXE交互作用和表观遗传效应及其对两年BP的影响。我们的项目通过对妇女和幼儿的健康和疾病进行基础研究，支持国家核研究所的使命“促进和改善个人、家庭、社区和人口的健康”。拟议的研究通过整合生物（遗传学）和行为（心理学）成分来扩展护理科学，这些成分可以告知导致美国HTN发病率最高的AA的多层次因素的组合。我们项目的发现有助于开发针对遗传和心理因素的干预措施，以降低HTN的风险。

DESCRIPTION (provided by applicant): This resubmission of a NEW INVESTIGATOR led longitudinal study is in response to (PA-11-104) and will examine: (1) the impact of (GXE) genetic and psychological environmental factors (discrimination, depression, and parenting behaviors) on the health of African American (AA) children aged 3 through 5 years and (2) the GXE risk for hypertension (HTN) in AA women and children. The overall goal of this project is to delineate the psychobiological (GXE interaction) mechanisms through which AA mothers' perceived racial discrimination, mental health, and parenting behavior affect their own and their young children's blood pressure (BP) over time. Our research will examine both genetic (candidate gene and epigenetic effects) and psychological (maternal perceived racial discrimination, mental health, and parenting behavior) interaction effects on BP on this population. We will employ a psychobiological approach by: utilizing psychological and biological assessments using a longitudinal cohort research design, a 2-step candidate gene and epigenetic methodology, and use of ancestry informative markers to account for population stratification admixture to explore GXE interactions on BP in AA mothers and children. Data analysis for the study will be conducted using multiple mixed modeling, cross-validation, and false discovery rate methods. We will enroll 250 AA children aged 3 through 5 years and their (n=250) mothers/maternal caregivers. We will assess mother and child factors every six months for 2 years. We propose the following: Aim 1: Examine the GXE interaction and epigenetic effects of mothers' perceived racial discrimination and its influence on BP over a period of two years. Aim 2: Examine the GXE interaction and epigenetic effects of mothers' mental health status [symptoms of depression] and its influence on BP over a period of two years. Aim 3: Examine the GXE interaction and epigenetic effects of mothers' parenting behavior and its influence on BP over a period of two years. Our project supports NINR's mission "to promote and improve the health of individuals, families, communities, and populations" by conducting basic research on the health and illness of women and young children. The proposed research extends nursing science by integrating the biological (genetics) and behavioral (psychology) components that can inform the combination of multi-level factors that contribute to AAs having the highest incidence of HTN in the US. Findings from our project can contribute to developing interventions that address genetic and psychological factors to reduce these risks for HTN.