Animal Tumor Models

动物肿瘤模型

• 批准号: 9113308
• 负责人: Vittorio Sebastiano
• 金额: $ 16.43万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9113308
• 关键词: Animals; Area; Budgets; CRISPR/Cas technology; Consultations; Cost Savings; Data Analyses; Data Collection; Doctor of Philosophy; Embryo; Faculty; Funding; Future; Generations; Goals; Histology; Histopathology; Housing; Immunohistochemistry; In Situ; Institutes; Knock-in; Knock-in Mouse; Knock-out; Knockout Mice; Malignant Neoplasms; Mediating; Medicine; Microinjections; Modeling; Mus; Pharmaceutical Preparations; Pilot Projects; Production; Rattus; Research Personnel; Resource Sharing; Running; Scientific Advances and Accomplishments; Services; Staining method; Stains; Technology; Therapeutic; Toxic effect; Transgenic Mice; Transgenic Organisms; animal resource; anticancer research; comparative; cost effective; embryonic stem cell; genome editing; member; mouse model; new technology; oncology; oncology service; pre-clinical; research and development; technology development; tissue processing; transcription activator-like effector nucleases; tumor; zygote

The Animal Tumor Models Shared Resource provides services in three major areas: i) transgenic and knockout mice, ii) preclinical oncology and iii) animal histopathology services. The primary goal of the Animal Tumor Models Shared Resource is to facilitate high-impact cancer research by Stanford Cancer Institute members by providing state-of-the-art and comprehensive animal resources for cancer studies. The transgenic and knockout mouse production services were established at Stanford in 1996 with the overall objective of providing genetically modified mouse models and supporting technologies to Stanford investigators at cost-effective rates. The preclinical oncology services, including tumor induction, drug administration, toxicity studies, data collection and analysis, were initiated by the SCI in 2007. These services are provided by the Transgenic, Knockout and Tumor Model Center (TKTC). The Veterinary Service Center (VSC) in the Department of Comparative Medicine runs the animal histopathology service component of the Shared Resource. Major services include tumor analysis by histology, immunohistochemistry and pathological interpretation. Substantial cost savings, efficiencies and scientific advances accrue from providing these services through this centralized facility. Vittorio Sebastiano, PhD, is the director of the shared resource. Barry Behr, MD, is the faculty advisor. Since 2009, over 85 SCI members have used the shared resource, which has an operating budget of $1.25 million/year. Future plans include enhancing the research and technology development at TKTC. This includes: i) generation of knockouts and knockin mouse models using TALENs and CRISPR/Cas9 mediated in situ genome editing in ES cells and mouse zygotes; ii) generation of knock-outs and knock-in rat models using TALENs and CRISPR/Cas9 mediated in situ genome editing in rat zygotes. These novel technologies will be offered in-house and we will also adapt new technologies developed elsewhere for the benefits of the SCI members.

动物肿瘤模型共享资源提供三个主要领域的服务：i）转基因和基因敲除小鼠，ii）临床前肿瘤学和iii）动物组织病理学服务。动物肿瘤模型共享资源的主要目标是通过为癌症研究提供最先进和全面的动物资源，促进斯坦福大学癌症研究所成员的高影响力癌症研究。1996年在斯坦福大学建立了转基因和基因敲除小鼠生产服务，其总体目标是以具有成本效益的价格向斯坦福大学研究人员提供转基因小鼠模型和支持技术。临床前肿瘤学服务，包括肿瘤诱导、药物给药、毒性研究、数据收集和分析，由SCI于2007年发起。这些服务由转基因，敲除和肿瘤模型中心（TKTC）提供。比较医学系的兽医服务中心（VSC）负责管理共享资源的动物组织病理学服务部分。主要服务包括肿瘤组织学分析、免疫组织化学和病理学解释。通过这一中央设施提供这些服务，可节省大量费用，提高效率，并取得科学进步。Vittorio Pastiano博士是共享资源的负责人。巴里贝尔，医学博士，是指导教师。自2009年以来，超过85个常设国际委员会成员使用了共享资源，其业务预算为125万美元/年。未来的计划包括加强TKTC的研究和技术开发。这包括：i）在ES细胞和小鼠受精卵中使用TALEN和CRISPR/Cas9介导的原位基因组编辑产生敲除和敲入小鼠模型;这些新技术将在内部提供，我们也将采用其他地方开发的新技术，以造福SCI成员。