Animal Tumor Models

动物肿瘤模型

• 批准号: 9113308
• 负责人: Vittorio Sebastiano
• 金额: $ 16.43万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9113308
• 关键词: Animals; Area; Budgets; CRISPR/Cas technology; Consultations; Cost Savings; Data Analyses; Data Collection; Doctor of Philosophy; Embryo; Faculty; Funding; Future; Generations; Goals; Histology; Histopathology; Housing; Immunohistochemistry; In Situ; Institutes; Knock-in; Knock-in Mouse; Knock-out; Knockout Mice; Malignant Neoplasms; Mediating; Medicine; Microinjections; Modeling; Mus; Pharmaceutical Preparations; Pilot Projects; Production; Rattus; Research Personnel; Resource Sharing; Running; Scientific Advances and Accomplishments; Services; Staining method; Stains; Technology; Therapeutic; Toxic effect; Transgenic Mice; Transgenic Organisms; animal resource; anticancer research; comparative; cost effective; embryonic stem cell; genome editing; member; mouse model; new technology; oncology; oncology service; pre-clinical; research and development; technology development; tissue processing; transcription activator-like effector nucleases; tumor; zygote

The Animal Tumor Models Shared Resource provides services in three major areas: i) transgenic and knockout mice, ii) preclinical oncology and iii) animal histopathology services. The primary goal of the Animal Tumor Models Shared Resource is to facilitate high-impact cancer research by Stanford Cancer Institute members by providing state-of-the-art and comprehensive animal resources for cancer studies. The transgenic and knockout mouse production services were established at Stanford in 1996 with the overall objective of providing genetically modified mouse models and supporting technologies to Stanford investigators at cost-effective rates. The preclinical oncology services, including tumor induction, drug administration, toxicity studies, data collection and analysis, were initiated by the SCI in 2007. These services are provided by the Transgenic, Knockout and Tumor Model Center (TKTC). The Veterinary Service Center (VSC) in the Department of Comparative Medicine runs the animal histopathology service component of the Shared Resource. Major services include tumor analysis by histology, immunohistochemistry and pathological interpretation. Substantial cost savings, efficiencies and scientific advances accrue from providing these services through this centralized facility. Vittorio Sebastiano, PhD, is the director of the shared resource. Barry Behr, MD, is the faculty advisor. Since 2009, over 85 SCI members have used the shared resource, which has an operating budget of $1.25 million/year. Future plans include enhancing the research and technology development at TKTC. This includes: i) generation of knockouts and knockin mouse models using TALENs and CRISPR/Cas9 mediated in situ genome editing in ES cells and mouse zygotes; ii) generation of knock-outs and knock-in rat models using TALENs and CRISPR/Cas9 mediated in situ genome editing in rat zygotes. These novel technologies will be offered in-house and we will also adapt new technologies developed elsewhere for the benefits of the SCI members.

动物肿瘤模型共享资源在三个主要领域提供服务：i) 转基因和基因敲除小鼠，ii) 临床前肿瘤学和 iii) 动物组织病理学服务。动物肿瘤模型共享资源的主要目标是通过为癌症研究提供最先进和全面的动物资源，促进斯坦福癌症研究所成员进行高影响力的癌症研究。转基因和基因敲除小鼠生产服务于 1996 年在斯坦福大学建立，总体目标是以具有成本效益的价格向斯坦福大学研究人员提供转基因小鼠模型和支持技术。 SCI于2007年启动了临床前肿瘤学服务，包括肿瘤诱导、给药、毒性研究、数据收集和分析。这些服务由转基因、基因敲除和肿瘤模型中心（TKTC）提供。比较医学系兽医服务中心 (VSC) 负责管理共享资源的动物组织病理学服务部分。主要服务包括肿瘤组织学分析、免疫组织化学和病理解释。通过这个集中设施提供这些服务，可以节省大量成本，提高效率并取得科学进步。 Vittorio Sebastiano 博士是共享资源的主管。 Barry Behr，医学博士，是教职顾问。自 2009 年以来，已有超过 85 个 SCI 成员使用了共享资源，该资源的运营预算为每年 125 万美元。未来的计划包括加强 TKTC 的研究和技术开发。这包括： i) 使用 TALEN 和 CRISPR/Cas9 在 ES 细胞和小鼠受精卵中介导的原位基因组编辑生成敲除和敲入小鼠模型； ii) 使用 TALEN 和 CRISPR/Cas9 介导的大鼠受精卵原位基因组编辑生成敲除和敲入大鼠模型。这些新技术将在内部提供，我们还将采用其他地方开发的新技术，以造福 SCI 成员。