Animal Tumor Models

动物肿瘤模型共享资源提供三个主要领域的服务：i)转基因和基因敲除小鼠，ii)临床前肿瘤学和iii)动物组织病理学服务。动物肿瘤模型共享资源的主要目标是通过为癌症研究提供最先进和全面的动物资源，促进斯坦福癌症研究所成员进行高影响力的癌症研究。转基因和基因敲除小鼠生产服务于1996年在斯坦福大学建立，总体目标是以符合成本效益的价格向斯坦福大学的研究人员提供转基因小鼠模型和支持技术。SCI于2007年启动了临床前肿瘤学服务，包括肿瘤诱导、药物给药、毒性研究、数据收集和分析。这些服务由转基因、基因敲除和肿瘤模型中心(TKTC)提供。比较医学系的兽医服务中心(VSC)运行共享资源的动物组织病理学服务部分。主要服务包括组织学肿瘤分析、免疫组织化学和病理解释。通过这一中央设施提供这些服务将大大节省成本、提高效率和取得科学进步。维托里奥·塞巴斯蒂亚诺博士是共享资源的负责人。医学博士巴里·贝尔是该校的教职顾问。自2009年以来，超过85个SCI成员使用了共享资源，其业务预算为125万美元/年。未来的计划包括加强将军澳隧道的研究和技术开发。这包括：i)使用TALENS和CRISPR/Cas9介导的ES细胞和小鼠受精卵的原位基因组编辑，建立敲除和敲除小鼠模型；ii)使用TALENS和CRISPR/Cas9介导的大鼠受精卵的原位基因组编辑，建立敲除和敲入的大鼠模型。这些新技术将在内部提供，我们还将调整其他地方开发的新技术，使SCI成员受益。

The Animal Tumor Models Shared Resource provides services in three major areas: i) transgenic and knockout mice, ii) preclinical oncology and iii) animal histopathology services. The primary goal of the Animal Tumor Models Shared Resource is to facilitate high-impact cancer research by Stanford Cancer Institute members by providing state-of-the-art and comprehensive animal resources for cancer studies. The transgenic and knockout mouse production services were established at Stanford in 1996 with the overall objective of providing genetically modified mouse models and supporting technologies to Stanford investigators at cost-effective rates. The preclinical oncology services, including tumor induction, drug administration, toxicity studies, data collection and analysis, were initiated by the SCI in 2007. These services are provided by the Transgenic, Knockout and Tumor Model Center (TKTC). The Veterinary Service Center (VSC) in the Department of Comparative Medicine runs the animal histopathology service component of the Shared Resource. Major services include tumor analysis by histology, immunohistochemistry and pathological interpretation. Substantial cost savings, efficiencies and scientific advances accrue from providing these services through this centralized facility. Vittorio Sebastiano, PhD, is the director of the shared resource. Barry Behr, MD, is the faculty advisor. Since 2009, over 85 SCI members have used the shared resource, which has an operating budget of $1.25 million/year. Future plans include enhancing the research and technology development at TKTC. This includes: i) generation of knockouts and knockin mouse models using TALENs and CRISPR/Cas9 mediated in situ genome editing in ES cells and mouse zygotes; ii) generation of knock-outs and knock-in rat models using TALENs and CRISPR/Cas9 mediated in situ genome editing in rat zygotes. These novel technologies will be offered in-house and we will also adapt new technologies developed elsewhere for the benefits of the SCI members.