Animal Tumor Models

动物肿瘤模型共享资源在三个主要领域提供服务：i)转基因和敲除小鼠，ii)临床前肿瘤学和iii)动物组织病理学服务。动物肿瘤模型共享资源的主要目标是通过为癌症研究提供最先进和全面的动物资源，促进斯坦福癌症研究所成员的高影响力癌症研究。转基因和基因敲除小鼠生产服务于1996年在斯坦福大学成立，其总体目标是以具有成本效益的价格为斯坦福大学的研究人员提供转基因小鼠模型和支持技术。临床前肿瘤学服务，包括肿瘤诱导、药物管理、毒性研究、数据收集和分析，由SCI于2007年启动。这些服务由转基因、基因敲除和肿瘤模型中心（TKTC）提供。比较医学系的兽医服务中心（VSC）负责管理共享资源的动物组织病理学服务部分。主要服务包括肿瘤组织学分析、免疫组织化学分析和病理解释。通过这个集中的设施提供这些服务，可以节省大量成本，提高效率并取得科学进步。Vittorio Sebastiano博士是共享资源的负责人。医学博士巴里·贝尔（Barry Behr）是指导教师。自2009年以来，已有超过85名SCI会员使用了共享资源，每年的运营预算为125万美元。未来的计划包括加强TKTC的研究和技术开发。这包括：i)在胚胎干细胞和小鼠受精卵中使用TALENs和CRISPR/Cas9介导的原位基因组编辑生成敲除和敲入小鼠模型；ii)利用TALENs和CRISPR/Cas9介导的大鼠受精卵原位基因组编辑技术建立敲除和敲入大鼠模型。这些新技术将在我们内部提供，我们也将采用其他地方开发的新技术，以使SCI会员受益。

The Animal Tumor Models Shared Resource provides services in three major areas: i) transgenic and knockout mice, ii) preclinical oncology and iii) animal histopathology services. The primary goal of the Animal Tumor Models Shared Resource is to facilitate high-impact cancer research by Stanford Cancer Institute members by providing state-of-the-art and comprehensive animal resources for cancer studies. The transgenic and knockout mouse production services were established at Stanford in 1996 with the overall objective of providing genetically modified mouse models and supporting technologies to Stanford investigators at cost-effective rates. The preclinical oncology services, including tumor induction, drug administration, toxicity studies, data collection and analysis, were initiated by the SCI in 2007. These services are provided by the Transgenic, Knockout and Tumor Model Center (TKTC). The Veterinary Service Center (VSC) in the Department of Comparative Medicine runs the animal histopathology service component of the Shared Resource. Major services include tumor analysis by histology, immunohistochemistry and pathological interpretation. Substantial cost savings, efficiencies and scientific advances accrue from providing these services through this centralized facility. Vittorio Sebastiano, PhD, is the director of the shared resource. Barry Behr, MD, is the faculty advisor. Since 2009, over 85 SCI members have used the shared resource, which has an operating budget of $1.25 million/year. Future plans include enhancing the research and technology development at TKTC. This includes: i) generation of knockouts and knockin mouse models using TALENs and CRISPR/Cas9 mediated in situ genome editing in ES cells and mouse zygotes; ii) generation of knock-outs and knock-in rat models using TALENs and CRISPR/Cas9 mediated in situ genome editing in rat zygotes. These novel technologies will be offered in-house and we will also adapt new technologies developed elsewhere for the benefits of the SCI members.