Dissecting the Molecular Role of TBX1 in the Context of Human Pharyngeal Endoderm Development

解析 TBX1 在人咽内胚层发育中的分子作用

• 批准号: 10615234
• 负责人: Vittorio Sebastiano
• 金额: $ 39.35万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10615234
• 关键词: 22q11.2; ATAC-seq; Affect; Anatomy; Animal Model; Arteries; Binding; Biocompatible Materials; Biology; Branchial arch structure; CRISPR interference; Cell Therapy; Cell model; Cells; ChIP-seq; Chromatin; Chromosome 22; Congenital Heart Defects; DNA; Data; Development; DiGeorge Syndrome; Disease; Dissection; Embryonic Development; Endoderm; Endoderm Cell; Endothelium; Enhancers; Ensure; Epigenetic Process; Face; Gene Deletion; Gene Expression; Generations; Genes; Genetic; Genetic Screening; Genetic Transcription; Heart; Heterozygote; Histones; Human; In Vitro; Intervention; Knowledge; Maps; Mesoderm; Methods; Modeling; Molecular; Mus; Muscle; Mutation; Neural Crest Cell; Nucleic Acid Regulatory Sequences; Parathyroid gland; Pathway interactions; Patients; Pattern; Pharyngeal Apparatus; Pharyngeal structure; Phase; Phenotype; Physiological; Play; Population; Qi; Regulation; Role; Signal Transduction; Structure; Syndrome; System; Testing; Thymus Gland; Tissues; Validation; Work; cell type; clinical phenotype; craniofacial; developmental disease; dosage; epigenetic regulation; experimental study; gene regulatory network; genetic variant; genome sequencing; human embryonic stem cell; human model; human tissue; in vitro Model; in vivo; insight; microdeletion; multidisciplinary; new technology; novel; pharmacologic; pharyngeal patterning; prevent; reconstruction; response; stem cell biology; tool; transcription factor; transcriptome; transcriptome sequencing; whole genome

PROJECT SUMMARY 22q11.2 Deletion Syndrome (22q11.2DS), the most common of the microdeletion syndromes, is caused by hemizygous loss of 0.7-3 Mb of DNA on chromosome 22 and results in a constellation of clinical phenotypes. The core phenotype originates from disrupted development of the pharyngeal apparatus. Particularly affected are the second heart field-dependent heart structures, great vessels, parathyroids, thymus, and lower craniofacial and face muscles. Although approximately 50 genes may be deleted, it is the haploinsufficiency of the transcription factor TBX1 that recapitulates most of the critical phenotype associated with 22q11.2DS. Genetic and developmental mouse studies have established that TBX1 is critical for typical development of the pharyngeal endoderm, a transient anatomical structure necessary for development of the thymus, parathyroids, and 4th pharyngeal arch arteries. Despite this central role, very little is known regarding the molecular mechanisms by which TBX1 functions in the pharyngeal endoderm. While a handful of studies have attempted to study the role of TBX1 in human cells, the cell types they have been conducted in are not representative of the appropriate developmental stage where and when TBX1 plays its critical role. To date, an effort to integrate all the critical genes into a pharyngeal endoderm or 4th pharyngeal arch arteries network has not been attempted, particularly in human cells. This R01 leverages recent development of an in vitro model which faithfully mimics the formation and progression of human pharyngeal endoderm, thereby providing an unprecedented opportunity to tease out the functions of TBX1 in its physiological context. Specifically, this model will be used to identify the transcriptional targets and partners of TBX1 (Aim1), investigate the role of TBX1 as epigenetic regulator of the human pharyngeal endoderm (Aim2), and mechanistically investigate newly discovered putative regulatory regions of the TBX1 locus (Aim3). The overarching hypothesis is that TBX1 is at the center of a Gene Regulatory Network critical for both the formation and maturation of the pharyngeal endoderm and the morpho-patterning of the surrounding mesoderm and neural crest cells. The proposed work is expected to identify the molecular mechanism at the basis of TBX1 haploinsufficiency and identify pathways which could be rescued through pharmacological intervention. Dissection of the epigenetic and molecular machinery responsible for pharyngeal endoderm formation will be instrumental in informing the generation of cell therapies for 22q11.2DS.

项目摘要 22q11.2缺失综合征（22q11.2DS）是最常见的微缺失综合征，由以下原因引起： 22号染色体上0.7-3 Mb DNA的半合子丢失，并导致一系列临床表型。 核心表型起源于咽器发育中断。特别影响 是第二心脏场依赖性心脏结构、大血管、甲状旁腺、胸腺和下 颅面和面部肌肉。虽然大约有50个基因可能被删除，但这是单倍不足， 转录因子TBX 1，其概括了与22q11.2DS相关的大多数关键表型。 遗传和发育小鼠研究已经确定，TBX 1对小鼠的典型发育至关重要。 咽内胚层是胸腺，甲状旁腺， 第四咽弓动脉尽管这一中心作用，很少有人知道关于分子 TBX 1在咽内胚层中发挥作用的机制。虽然有一些研究试图 为了研究TBX 1在人类细胞中的作用，他们所进行的细胞类型并不代表 TBX 1在何时何地发挥其关键作用的适当发育阶段。迄今为止， 尚未尝试将所有关键基因导入咽内胚层或第四咽弓动脉网， 特别是在人类细胞中。 该R 01利用了最近开发的一种体外模型，该模型忠实地模拟了 人咽部内胚层，从而提供了一个前所未有的机会，梳理出的功能， TBX 1在其生理背景下。具体而言，该模型将用于鉴定转录靶点， TBX 1（Aim 1）的合作伙伴，研究TBX 1作为人咽内胚层表观遗传调节因子的作用 （Aim 2），并机械地研究新发现的TBX 1基因座的推定调控区（Aim 3）。 总体假设是TBX 1处于基因调控网络的中心，该网络对基因和蛋白质都至关重要。 咽内胚层的形成和成熟以及周围中胚层的形态模式 和神经嵴细胞。 这项工作有望在TBX 1单倍不足的基础上确定分子机制， 确定可以通过药物干预挽救的途径。表观遗传学的剖析 负责咽内胚层形成的分子机制将有助于告知 22q11.2DS的细胞疗法。