Fluidigm BioMark HD and Juno integrated system

Fluidigm BioMark HD 和 Juno 集成系统

• 批准号: 9076066
• 负责人: David Eric Symer
• 金额: $ 27万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-02-15 至 2017-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9076066
• 关键词: Alternative Splicing; Area; Basic Science; Biological Assay; Clinical Research; Communities; Comprehensive Cancer Center; Custom; DNA copy number; Data; Diagnosis; Disease; End Point Assay; Funding; Genomic DNA; Genomics; Genomics Shared Resource; Genotype; Grant; Health; Human; Institution; Laboratories; Methods; MicroRNAs; Microfluidics; Molecular; Ohio; Oligonucleotides; Pilot Projects; Polymerase Chain Reaction; Proteins; Research; Research Personnel; Research Project Grants; Sampling; Services; System; Technology; Transcript; United States National Institutes of Health; Universities; Validation; Variant; anticancer research; base; design; digital; flexibility; genomic data; genomic platform; high throughput screening; human disease; improved; insight; instrument; instrumentation; member; next generation sequencing; prevent; programs; research study; scale up

 DESCRIPTION (provided by applicant) Rapidly evolving genomics technologies have driven remarkable research breakthroughs which are now beginning to explain the molecular basis for many human diseases as well as natural variation. The Genomics Shared Resource (GSR) of the Ohio State University Comprehensive Cancer Center has remained abreast of these evolving methods by providing complementary and cutting-edge genomics services to a broad community of investigators. These services involve sequencing, hybridization and/or polymerase chain reaction (PCR)-based methods. GSR staff members help investigators to design genomics experiments and generate high quality genomics data to support their basic and clinical research projects. This core laboratory primarily supports cancer research at Ohio State, but also routinely serves other research areas at Ohio State and at other institutions. In this S10 Shared Instrumentation Grant Program proposal, the GSR proposes to acquire a flexible, highly scalable, integrated microfluidics system that facilitates custom and/or high-throughput assays using various forms of PCR including quantitative PCR, end-point assays and digital PCR. This integrated system, called the Fluidigm Juno loader and BioMark HD, will allow the GSR to perform PCR-based assays for genotyping, quantitation of transcript expression levels including microRNAs and alternative RNA splicing, genomic DNA copy number changes, and even oligonucleotide-tagged proteins. A particular advantage of the requested system is that it is highly scalable, so the same instrument and experimental conditions for small pilot projects can be scaled up in a straightforward and simple way to hundreds of assays and many thousands of samples (or even more). This integrated Fluidigm system will allow for rapid and economical validation of data generated by other genomics platforms including next generation sequencing (NGS). Its addition to the GSR will support and enhance ongoing NIH- funded research projects. This is very likely to result in new insights into human diseases and thereby create new opportunities to diagnose, treat and prevent diseases and to improve human health.

 描述（由申请人提供） 快速发展的基因组学技术推动了显着的研究突破，现在开始解释许多人类疾病以及自然变异的分子基础。俄亥俄州州立大学综合癌症中心的基因组学共享资源（GSR）通过向广泛的研究人员社区提供补充和尖端的基因组学服务，一直与这些不断发展的方法保持同步。这些服务包括测序、杂交和/或基于聚合酶链反应（PCR）的方法。GSR的工作人员帮助研究人员设计基因组学实验，并生成高质量的基因组学数据，以支持他们的基础和临床研究项目。该核心实验室主要支持俄亥俄州的癌症研究，但也经常为俄亥俄州和其他机构的其他研究领域提供服务。在这项S10共享仪器资助计划提案中，GSR提议获得一种灵活的、高度可扩展的集成微流体系统，该系统使用各种形式的PCR（包括定量PCR、终点测定和数字PCR）促进定制和/或高通量测定。这种集成系统称为Fluidigm Juno加载器和BioMark HD，将允许GSR进行基于PCR的基因分型分析，定量转录表达水平，包括microRNA和替代RNA剪接，基因组DNA拷贝数变化，甚至是标记的蛋白质。所要求的系统的一个特别优点是它是高度可扩展的，因此用于小型试点项目的相同仪器和实验条件可以以直接和简单的方式扩展到数百个测定和数千个样品（甚至更多）。这种集成的Fluidigm系统将允许快速和经济地验证其他基因组学平台生成的数据，包括下一代测序（NGS）。它加入GSR将支持和加强正在进行的NIH资助的研究项目。这很可能导致对人类疾病的新认识，从而为诊断、治疗和预防疾病以及改善人类健康创造新的机会。