Genomics Shared Resource

CORE-015：基因组学共享资源（GSR） 项目总结/摘要 基因组学服务，其广泛的不同技术和复杂性，是必不可少的癌症 research.由于仪器的费用，需要集中资源进行这些分析 和必要的专业知识OSUCCC基因组学共享资源（GSR）是一个最先进的实验室 具有以下特定目的：1）使用下一代测序技术对DNA和RNA模板进行测序， 测序平台（即Illumina MiSeq和HiSeq 2500，以及Ion Torrent PGM仪器）和毛细管电泳仪 桑格测序和基因分型（使用ABI 3730 DNA分析仪）; 2）使用敏感的分子生物学技术， 检测和定量RNA转录物表达水平和结构的杂交方法， 和/或DNA拷贝数和变异，包括数字（Nanostring）和最先进的微阵列 （亲和素）平台;和，3）进行基于聚合酶链反应（PCR）的扩增以检测， 定量并确认拷贝数变异、单核苷酸变异和小插入/缺失 多态性，包括定量PCR（ABI 3730 DNA分析仪）和高通量数字和 定制PCR测定（QuantStudio 12 K Flex和OpenArray）方法。GSR被整合为一个单一的 2012年，OSUCCC微阵列共享资源和核酸共享资源 一起来提高效率并便于用户容易地进行测定和选择。主任是大卫西默博士 （MBCG）和高级教师顾问是卡罗克罗齐博士（MBCG）。GSR位于生物医学 研究塔（BRT），因此位于大多数OSUCCC实验室的中心。在上一次拨款中 在此期间，OSUCCC购买了新的设备和/或升级，以保持在基因组学的前沿 研究，总计3，110，511美元。作为衡量海矿资源公司提供的服务对 在上一个资助期内，GSR为175个OSUCCC提供了基因组学服务 成员共有375篇出版物纳入了GSR服务，其中51篇具有期刊影响因子 大于10 μGSR支持了104项NCI赠款，包括16项计划赠款（即，N01，P50， P01，U10，U 54），57个R 01和20个R21。在未来五年内，GSR将继续更新其 技术平台和基因组学方法的专业知识，并将继续创新新的检测方法， 在这个快节奏的研究领域的方法。未来的计划包括实施新的方法， 机器人系统的长读序测序和采集，以生成可重复的高质量文库， 测序从技术上具有挑战性和更有限的起始材料制备。GSR利用 广泛的机构支持，仅寻求CCSG基金的22.1%的支持。枪击残留物是 诊断学

CORE-015: GENOMICS SHARED RESOURCE (GSR) PROJECT SUMMARY / ABSTRACT Genomics services, with its wide range of varying technologies and complexities, are essential for cancer research. A centralized resource is needed to conduct these analyses due to the expense of the instruments and the requisite expertise. The OSUCCC Genomics Shared Resource (GSR) is a state-of-the-art laboratory that has the following Specific Aims: 1) to sequence DNA and RNA templates, using next generation sequencing platforms (i.e. Illumina MiSeq and HiSeq 2500, and Ion Torrent PGM instruments) and capillary Sanger sequencing and genotyping (using ABI 3730 DNA Analyzers); 2) to use sensitive molecular hybridization methods to detect and quantify RNA transcript expression levels and structures such as splicing and/or DNA copy numbers and variation, including digital (Nanostring) and state-of-the-art microarray (Affymetrix) platforms; and, 3) to perform polymerase chain reaction (PCR)-based amplification to detect, quantify and confirm copy number variants, single nucleotide variants and small insertion/deletion polymorphisms including quantitative PCR (ABI 3730 DNA Analyzer) and by high throughput digital and custom PCR assay (QuantStudio 12K Flex and OpenArray) methods. The GSR was integrated as a single entity in 2012, bringing the prior OSUCCC MicroArray Shared Resource and Nucleic Acid Shared Resource together to enhance efficiency and facilitate user ease and choice of assays. The Director is Dr. David Symer (MBCG) and the Senior Faculty Advisor is Dr. Carlo Croce (MBCG). The GSR is located in the Biomedical Research Tower (BRT) and so is centrally located to most of the OSUCCC laboratories. Over the last grant period, the OSUCCC purchased new equipment and/or upgrades to stay on the cutting edge of genomics research, totaling $3,110,511. As a measure of the highly significant value of services provided by the GSR to OSUCCC researchers over the last grant period, the GSR provided genomics services to 175 OSUCCC members. A total of 375 publications incorporated GSR services of which 51 had a journal impact factor greater than 10. The GSR has supported 104 NCI grants, including 16 programmatic grants (i.e., N01, P50, P01, U10, U54), 57 R01s and 20 R21s. Over the next five year period, the GSR will continue to update its technological platforms and expertise in genomics methods and will continue to innovate with new assays and approaches in this very fast-paced area of research. Future plans include implementation of new methods in long-read sequencing and acquisition of robotic systems to generate reproducible high quality libraries for deep sequencing prepared from technically challenging and more limited starting materials. The GSR leverages extensive institutional support and seeks only 22.1% support from CCSG funds. The GSR is part of the Diagnostics Grouping.