Mouse Behavioral Assessment and Breeding Core

instmctions）; 小鼠行为评估和育种核心是以前支持的核心的扩展。 程序.核心有许多支持计划中所有项目的具体任务。的 由经验丰富的核心人员承担的具体任务如下：a）唤醒的行为评估 和睡眠阶段。在前一时期， 资助我们开发了一种新的方法来评估唤醒和睡眠阶段的视频分析; 2） 用脑电图和肌电图记录清醒和睡眠。这涉及植入 电极和记录的仔细评分。核心人员还对EEG信号进行频谱分析; 3） 这些研究产生了大量的行为数据。因此，核心也进行数据缩减 并使用我们开发的定制软件，以易于理解的方式向调查人员提供汇总数据。 访问格式; 4）这项新的研究计划现在涉及一些 特定的转基因小鼠建议这最好由中央设施完成，因此， 提供小鼠的繁殖和基因分型。虽然这些都是核心部门正在提供的增强型服务， 此外，该中心还从事创新活动。首先，核心工作人员将对变化进行研究 在C57 BL/6 NIA小鼠的整个寿命中的睡眠/觉醒中。这些小鼠用于项目01和02。因此，在本发明中， 这些数据将为这两个项目的科学研究提供信息。核心还解决了创建 新的转基因小鼠作为第一步，我们建议创建条件转基因， 在老年动物中，Homer la可以增加。这是项目02的近期方向。最后 Core将与Core C的工作人员合作，努力扩展基于视频的技术，以便唤醒和 可以在一起饲养的一组小鼠中的单个小鼠中评估睡眠阶段。 相关性（见说明）： 小鼠是研究睡眠和衰老影响的强大模型系统。老鼠在清醒时也表现出同样的变化 像人类一样睡觉这个核心提供了研究所有年龄段小鼠睡眠和觉醒的能力， 这项研究所需的行为评估它还将创造出突变小鼠， 研究特定基因改变的小鼠并负责小鼠育种的能力。

instmctions); The Mouse Behavioral Assessment and Breeding Core is an extension of a core in the previously supported program. The Core has a number of specific tasks that support all of the projects in the program. The specific tasks undertaken by the experienced Core staff are the following: a) behavioral assessment of wake and sleep stages in mice using high throughput video assessment of behavior. In the previous period of funding we developed a novel methodology to assess wake and stages of sleep from video analysis; 2) recording of wake and sleep using electroencephalogram and electromyogram. This involves implantation of electrodes and careful scoring of records. Core staff also perform spectral analysis of the EEG signal; 3) these studies generate a considerable amount of behavioral data. Thus, the Core also does data reduction and, using customized software we have developed, provides summary data to investigators in an easy to access format; 4) this new program of research now involves breeding and genotyping of a number of specific transgenic mice. It is proposed that this is best done by a central facility and therefore this Core now provides breeding of mice and genotyping. While these are ongoing and enhanced services that the Core will provide, the Core is also engaged in innovative activities. First, Core staff will conduct a study of changes in sleep/wake across the lifespan in C57BL/6NIA mice. These mice are used in Projects 01 and 02. Thus, these data will inform the science of these two projects. The Core is also addressing the capability to create new transgenic mice. As a first step, we proposed to create conditional transgenics where expression of Homer l a can be increased in older animals. This is an immediate future direction for Project 02. Finally, the Core will work to extend, in collaboration with staff in Core C, the video-based technology so that wake and stages of sleep can be assessed in individual mice among a group of mice that are raised together. RELEVANCE (See Instmctions): Mice are a powerful model system to study sleep and aging effects. Mice show the same changes in wake and sleep as humans. This Core provides the ability to study sleep and wake in mice of all ages to provide the behavioral assessments needed for this program of research. It also will create mutant mice that provide the capability to study mice with alteration in a particular gene and be responsible for mouse breeding.