Mouse Behavioral Assessment and Breeding Core

说明)； 小鼠行为评估和繁育核心是先前支持的 程序。核心有许多具体的任务来支持计划中的所有项目。这个 有经验的核心工作人员承担的具体任务如下：a)唤醒行为评估 并使用高通量视频对小鼠的睡眠阶段行为进行评估。在前一段时间 资助我们开发了一种新的方法，通过视频分析来评估觉醒和睡眠阶段；2) 用脑电和肌电记录觉醒和睡眠。这涉及到植入 电极和仔细的记录评分。核心工作人员还对脑电信号进行频谱分析；3) 这些研究产生了大量的行为数据。因此，核心还执行数据缩减 而且，使用我们开发的定制软件，可以轻松地向调查人员提供摘要数据 4)这项新的研究计划现在涉及到一些品种的培育和基因分型 特定的转基因小鼠。建议最好由一个中央设施来完成这项工作，因此该核心现在 提供小鼠的饲养和基因分型。虽然这些都是持续和增强的服务，但核心 将提供，核心也在从事创新活动。首先，核心员工将对变化进行研究 在C57BL/6NIA小鼠的整个生命周期中处于睡眠/清醒状态。这些鼠标在项目01和02中使用。因此， 这些数据将为这两个项目的科学提供信息。核心还解决了创建 新的转基因小鼠。作为第一步，我们建议创建有条件的转基因，其中表达 荷马L a可在年长的动物中增加。这是Project 02近期的未来方向。最后， Core将与Core C的员工合作，扩展基于视频的技术，以便唤醒和 在一起饲养的一组小鼠中，可以对单个小鼠的睡眠阶段进行评估。 相关性(请参阅实例)： 小鼠是研究睡眠和衰老影响的一个强大的模型系统。小鼠在尾流中表现出同样的变化 像人类一样睡觉。这个核心提供了研究所有年龄的小鼠的睡眠和醒来的能力，以提供 这一研究项目所需的行为评估。它还将创造出突变的小鼠，为 研究特定基因突变的小鼠并负责培育小鼠的能力。

instmctions); The Mouse Behavioral Assessment and Breeding Core is an extension of a core in the previously supported program. The Core has a number of specific tasks that support all of the projects in the program. The specific tasks undertaken by the experienced Core staff are the following: a) behavioral assessment of wake and sleep stages in mice using high throughput video assessment of behavior. In the previous period of funding we developed a novel methodology to assess wake and stages of sleep from video analysis; 2) recording of wake and sleep using electroencephalogram and electromyogram. This involves implantation of electrodes and careful scoring of records. Core staff also perform spectral analysis of the EEG signal; 3) these studies generate a considerable amount of behavioral data. Thus, the Core also does data reduction and, using customized software we have developed, provides summary data to investigators in an easy to access format; 4) this new program of research now involves breeding and genotyping of a number of specific transgenic mice. It is proposed that this is best done by a central facility and therefore this Core now provides breeding of mice and genotyping. While these are ongoing and enhanced services that the Core will provide, the Core is also engaged in innovative activities. First, Core staff will conduct a study of changes in sleep/wake across the lifespan in C57BL/6NIA mice. These mice are used in Projects 01 and 02. Thus, these data will inform the science of these two projects. The Core is also addressing the capability to create new transgenic mice. As a first step, we proposed to create conditional transgenics where expression of Homer l a can be increased in older animals. This is an immediate future direction for Project 02. Finally, the Core will work to extend, in collaboration with staff in Core C, the video-based technology so that wake and stages of sleep can be assessed in individual mice among a group of mice that are raised together. RELEVANCE (See Instmctions): Mice are a powerful model system to study sleep and aging effects. Mice show the same changes in wake and sleep as humans. This Core provides the ability to study sleep and wake in mice of all ages to provide the behavioral assessments needed for this program of research. It also will create mutant mice that provide the capability to study mice with alteration in a particular gene and be responsible for mouse breeding.