The HIV-1 Tat protein is an reverse transcription co-factor.

HIV-1 Tat 蛋白是一种逆转录辅助因子。

• 批准号: nhmrc : 389826
• 负责人: A/Pr David Harrich
• 金额: $ 26.98万
• 依托单位: Queensland Institute of Medical Research
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/hiv-1-tat-co-factor/81474
• 关键词: HIV; Tat; protein; reverse; transcription

HIV-1 is the virus that causes AIDS. In order for HIV-1 to grow, the viral genetic material must be converted into a form that is compatible with a human host. Specifically, the HIV-1 genetic material is made of RNA while the human genome is composed of DNA. An HIV-1 enzyme called reverse transcriptase (RT) is used for this purpose. We have discovered that another HIV-1 protein called Tat is also required for the efficient conversion of HIV-1 RNA into HIV-1 DNA. If HIV-1 lacks Tat, then this transformation process is inefficient and HIV-1 is not able to grow. Recently our group made a breakthrough discovery on how Tat works. Tat can directly bind to RT and stimulate the conversion process. This research is aimed at a detailed analysis of Tat and RT interaction. This information is required in order to understand how this interaction can be blocked in order to stop HIV-1 growth. In the long-term, results produced by this research will be required to discover novel drugs to combat HIV-AIDS.

HIV-1是导致艾滋病的病毒。为了使HIV-1生长，病毒遗传物质必须转化为与人类宿主相容的形式。具体而言，HIV-1遗传物质由RNA组成，而人类基因组由DNA组成。一种称为逆转录酶（RT）的HIV-1酶用于此目的。我们发现，另一种称为达特的HIV-1蛋白质也是HIV-1 RNA有效转化为HIV-1 DNA所必需的。如果HIV-1缺乏达特，那么这种转化过程是低效的，HIV-1就不能生长。最近，我们小组在达特的工作原理上有了突破性的发现。达特可以直接与RT结合并刺激转化过程。本研究旨在详细分析达特和RT的相互作用。为了了解如何阻断这种相互作用以阻止HIV-1的生长，需要这些信息。从长远来看，将需要这项研究的结果来发现对抗艾滋病毒/艾滋病的新药。