Microbial and cell culture analytical system

拟议的设备，台式流式细胞仪(带有侧面散射升级和高级检测套件的番石榴EasyCyte Mini)，能够测量细胞浓度、细胞活力、细胞周期时相和细胞凋亡。它还能够检测细胞产生的生物分子，如蛋白质。拟议的设备将用于测量种群中的细胞密度和活力。此外，它将被用来根据细胞周期或在细胞凋亡中的位置将种群中的细胞分配到亚群，并评估细胞的相对蛋白质生产率。所有正在生长的细胞种群将被安置在拟议的二氧化碳孵化器中，并将储存在拟议的超低温冷冻箱中。等待用台式流式细胞仪进行分析的样本也将储存在拟议的超低温冷冻室中。所有通过使用拟议的台式流式细胞仪进行的分析收集的数据将用于开发培养系统的模型和制定培养优化策略。从哺乳动物细胞培养中产生的多种蛋白质代表着一种快速增长的生物制药类别。用于高度可重复性的哺乳动物细胞培养系统的高度合格的个人和稳健的模型和优化策略仍然是需求。此外，随着市场的不断扩大，动物细胞技术方面的HQP人才日益短缺。由拟议的设备实现的HQP的研究和培训与生物制药行业和整个生物技术行业高度相关。

The proposed equipment, a desk top flow cytometer (the Guava EasyCyte Mini with Side Scatter Upgrade and Advanced Assay Suite), is capable of measuring cell concentration, cell viability, cell cycle phase, and apoptosis. It is also capable of detecting biomolecules, such as proteins, produced by the cells. The proposed equipment will be used to measure cell concentration and viability within a population. Further, it will be used to assign the cells in the population to subpopulations on the basis of position in the cell cycle or in apoptosis, and to assess the relative protein productivity of the cells.    Growing cell populations will be housed in the proposed carbon dioxide incubator and will be stored in the proposed ultra low temperature freezer. Samples awaiting analysis with the desk top flow cytometer will also be stored in the proposed ultra low temperature freezer.    Data collected through assays performed with the proposed desk top flow cytometer will be used to develop models of the culture system and to develop culture optimization strategies.    Proteins produced from mammalian cell culture represent a rapidly growing class of biopharmaceuticals. Both highly qualified personal and robust models and optimization strategies for highly reproducible mammalian cell culture systems continue to be in demand. Moreover, as the market continues to expand, a growing shortage of HQP skilled in animal cell technology has developed. The research and the training of HQP enabled by the proposed equipment are highly relevant to the biopharmaceutical industry and to the biotechnology industry in general.