Microbial and cell culture analytical system

微生物和细胞培养分析系统

• 批准号: 345545-2007
• 负责人: Dutton, Roshni
• 金额: $ 6.89万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325643
• 关键词: Microbial; cell; culture; analytical; system

The proposed equipment, a desk top flow cytometer (the Guava EasyCyte Mini with Side Scatter Upgrade and Advanced Assay Suite), is capable of measuring cell concentration, cell viability, cell cycle phase, and apoptosis. It is also capable of detecting biomolecules, such as proteins, produced by the cells. The proposed equipment will be used to measure cell concentration and viability within a population. Further, it will be used to assign the cells in the population to subpopulations on the basis of position in the cell cycle or in apoptosis, and to assess the relative protein productivity of the cells.    Growing cell populations will be housed in the proposed carbon dioxide incubator and will be stored in the proposed ultra low temperature freezer. Samples awaiting analysis with the desk top flow cytometer will also be stored in the proposed ultra low temperature freezer.    Data collected through assays performed with the proposed desk top flow cytometer will be used to develop models of the culture system and to develop culture optimization strategies.    Proteins produced from mammalian cell culture represent a rapidly growing class of biopharmaceuticals. Both highly qualified personal and robust models and optimization strategies for highly reproducible mammalian cell culture systems continue to be in demand. Moreover, as the market continues to expand, a growing shortage of HQP skilled in animal cell technology has developed. The research and the training of HQP enabled by the proposed equipment are highly relevant to the biopharmaceutical industry and to the biotechnology industry in general.

所提议的设备是台式流式细胞仪（具有侧散射升级和高级分析套件的 Guava EasyCyte Mini），能够测量细胞浓度、细胞活力、细胞周期阶段和细胞凋亡。它还能够检测细胞产生的生物分子，例如蛋白质。拟议的设备将用于测量群体内的细胞浓度和活力。此外，它将用于根据细胞周期或细胞凋亡中的位置将细胞群中的细胞分配到亚群，并评估细胞的相对蛋白质生产力。    生长中的细胞群将被安置在拟议的二氧化碳培养箱中，并将储存在拟议的超低温冰箱中。等待用台式流式细胞仪分析的样品也将存储在建议的超低温冰箱中。    通过使用所提议的台式流式细胞仪进行的测定收集的数据将用于开发培养系统的模型并制定培养优化策略。    由哺乳动物细胞培养物产生的蛋白质代表了一类快速增长的生物制药。对于高度可重复的哺乳动物细胞培养系统，高质量的个人和稳健的模型以及优化策略仍然存在需求。此外，随着市场的不断扩大，动物细胞技术的总部人员日益短缺。拟议设备所支持的 HQP 研究和培训与生物制药行业和整个生物技术行业高度相关。