Characterization, modeling and environmental manipulation of mammalian cell culture systems for optimal protein production

哺乳动物细胞培养系统的表征、建模和环境操作，以实现最佳蛋白质生产

• 批准号: 346131-2007
• 负责人: Dutton, Roshni
• 金额: $ 1.6万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=430348
• 关键词: Characterization; modeling; environmental; manipulation; mammalian

Mammalian cell culture comprises a major platform for producing a number of high-value biopharmaceuticals, including enzymes, monoclonal antibodies (MAbs), viral vaccines, hormones and immuno-regulators. The future of the biopharmaceutical industry will rely on the development and enhancement of these mammalian cell culture production systems. Such biologics are produced in relatively small quantities due to the highly specialized culture conditions needed and the susceptibility of the culture to either reduced productivity or significant cell death as a result of slight changes in culture conditions. While earlier generations of biochemical engineers focused on the non-biological aspects of production, such as reactor design, more recent engineering developments have recognized that the greatest potential for enhancing production lies within the animal cells themselves.

哺乳动物细胞培养是生产许多高价值生物药物的主要平台，包括酶、单抗、病毒疫苗、激素和免疫调节剂。生物制药行业的未来将依赖于这些哺乳动物细胞培养生产系统的发展和增强。这种生物制品的产量相对较小，因为所需的培养条件高度专业化，而且由于培养条件的轻微变化，培养物对生产率降低或细胞严重死亡的敏感性较高。虽然前几代生化工程师专注于生产的非生物方面，如反应堆设计，但最近的工程发展已经认识到，提高产量的最大潜力在于动物细胞本身。