Characterization, modeling and environmental manipulation of mammalian cell culture systems for optimal protein production

哺乳动物细胞培养包括用于生产许多高价值生物药物的主要平台，所述高价值生物药物包括酶、单克隆抗体（MAb）、病毒疫苗、激素和免疫调节剂。生物制药行业的未来将依赖于这些哺乳动物细胞培养生产系统的开发和增强。由于所需的高度专业化的培养条件以及培养物对由于培养条件的轻微变化而导致的生产率降低或显著细胞死亡的敏感性，此类生物制剂以相对小的量生产。虽然早期的生化工程师专注于生产的非生物方面，如反应器设计，但最近的工程发展已经认识到，提高产量的最大潜力在于动物细胞本身。

Mammalian cell culture comprises a major platform for producing a number of high-value biopharmaceuticals, including enzymes, monoclonal antibodies (MAbs), viral vaccines, hormones and immuno-regulators. The future of the biopharmaceutical industry will rely on the development and enhancement of these mammalian cell culture production systems. Such biologics are produced in relatively small quantities due to the highly specialized culture conditions needed and the susceptibility of the culture to either reduced productivity or significant cell death as a result of slight changes in culture conditions. While earlier generations of biochemical engineers focused on the non-biological aspects of production, such as reactor design, more recent engineering developments have recognized that the greatest potential for enhancing production lies within the animal cells themselves.