Fluorimeter upgrade

荧光计升级

• 批准号: 344472-2007
• 负责人: Bohne, Cornelia
• 金额: $ 0.94万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325865
• 关键词: Fluorimeter; upgrade

Fluorescence is a property where molecules emit light after absorbing light of a different color (higher energy, shorter wavelength).  Fluorescence measurements are very sensitive and at its limit emission can be detected from a single molecule.  Because of its sensitivity fluorescence has found many applications, as for example in sensors in medical diagnostic kits.  Chemists use fluorescence as a diagnostic tool for how efficiently reactions happen and as probes to understand how molecules can interact with one another.  In the Bohne group fluorescence is an essential tool to establish how guests bind to supramolecular systems.  This characterization precedes all of the kinetic studies to determine the binding dynamics of guests to host systems. In addition, the expertise and ability for fluorescence studies of complex systems has been the central theme for the collaboration of the Bohne group with researchers in Japan and Germany. The Wan group specializes in studying how photochemical reactions happen.  Many of the compounds after absorbing light (initiation process for photochemistry) and before reacting undergo fluorescence and this emission is used as a diagnostic tool for the efficiency of the reactions. The current fluorimeter used by both research groups is 10 years old and data acquisition and analysis is performed on an old computer with proprietary DOS software.  The request for an upgrade would eliminate the bottleneck in the acquisition of fluorescence data and would enhance the productivity of the groups involved.

荧光是分子吸收不同颜色的光后发出光的性质（更高的能量，更短的波长）。荧光测量是非常灵敏的，并且在其极限下可以从单个分子检测到发射。由于其灵敏度，荧光已经发现了许多应用，例如，在医疗诊断试剂盒中的传感器中。化学家使用荧光作为诊断工具，以了解反应如何有效地发生，并作为探针来了解如何分子可以相互作用。在Bohne群中，荧光是确定客体如何与超分子系统结合的重要工具。这种表征先于所有动力学研究，以确定客体与主体系统的结合动力学。此外，复杂系统荧光研究的专业知识和能力一直是Bohne集团与日本和德国研究人员合作的中心主题。Wan小组专门研究光化学反应是如何发生的。许多化合物在吸收光（光化学的起始过程）后和反应前会发生荧光，这种发射被用作反应效率的诊断工具。两个研究小组目前使用的荧光计已经使用了10年，数据采集和分析是在一台装有专有DOS软件的旧计算机上进行的，要求升级将消除采集荧光数据的瓶颈，并将提高有关小组的生产力。