Electrochemical - surface plasmon resonance studies of thiol decorated nanoparticles as biosensors

硫醇修饰纳米粒子作为生物传感器的电化学-表面等离子共振研究

• 批准号: 345845-2007
• 负责人: Shepherd, Jeffrey
• 金额: $ 2.75万
• 依托单位: Laurentian University of Sudbury
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=325977
• 关键词: Electrochemical; surface; plasmon; resonance; studies

The equipment requested in this grant is a Surface Plasmon Resonance (SPR) Spectrometer that will ultimately be used in biosensing. Biosensors are a class of device engineered to recognize a target biomolecule in a given environment. The medical community has relied on these sensors for early detection and treatment of disease but biosensors are becoming increasingly attractive for the detection of biological warfare agents. As such, the attributes of a sensor often include miniaturized size, high specificity, multi-functionality and the absence of false positives. With the ever changing requirements of a sensor, new methods for the creation and characterization of the material are ongoing. Typically the bio-recognition event is based on molecular binding with a substrate and surface modification strategies exist to functionalize the material with specific receptor groups. To enhance possible multi-functionality, a variety of receptor groups is desirable and made possible through surface patterning methods. Our research focuses on the use of electrochemical techniques to uniquely design and characterize a surface coated with organic-receptor materials in a patterned geometry. We will combine our experience with surface patterning of bulk surfaces to decorate gold nanoparticles in a similar manner. Coupling the nanoparticles with SPR spectroscopy will enhance our detection schemes beyond typical SPR investigations. The creation of new and improved biosensors may lead to new detection limits and earlier diagnosis and treatment of disease thus having a positive impact on the health and well being of society.

本资助申请的设备是一台表面等离子体共振（SPR）光谱仪，最终将用于生物传感。生物传感器是一类被设计成在给定环境中识别目标生物分子的装置。医学界依赖这些传感器来早期检测和治疗疾病，但生物传感器在检测生物战剂方面正变得越来越有吸引力。因此，传感器的属性通常包括小型化尺寸、高特异性、多功能性和不存在假阳性。随着传感器需求的不断变化，用于材料的创建和表征的新方法正在进行中。通常，生物识别事件是基于与底物的分子结合，并且存在表面改性策略以用特定受体基团官能化材料。为了增强可能的多功能性，需要多种受体基团，并通过表面图案化方法使其成为可能。我们的研究重点是使用电化学技术来独特地设计和表征涂覆有图案化几何形状的有机受体材料的表面。我们将结合联合收割机我们的经验与表面图案的散装表面装饰金纳米粒子以类似的方式。耦合的纳米粒子与SPR光谱将提高我们的检测方案超越典型的SPR调查。新的和改进的生物传感器的创建可能导致新的检测极限和早期诊断和治疗疾病，从而对社会的健康和福祉产生积极影响。