Engineering defects in SAMs of alkylthiols for improved biosensing strategies

烷基硫醇 SAM 的工程缺陷可改善生物传感策略

• 批准号: 341979-2007
• 负责人: Shepherd, Jeffrey
• 金额: $ 1.71万
• 依托单位: Laurentian University of Sudbury
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=361503
• 关键词: Engineering; defects; SAMs; alkylthiols; improved

Biosensors are a class of device engineered to recognize a target biomolecule in a given environment. They have been used in the medical community for the early detection and treatment of disease but are also desirable as methods of detecting biological warfare agents. As such, the attributes of a sensor often include minaturized size, high specificity, multi-functionality and the absence of false positives. With the ever changing requirements of the sensor, new methods for the creation and characterization of the sensor material are ongoing. Typically the bio-recognition event is based on molecular binding with a substrate and surface modification strategies exist to functionalize the material with specific receptor groups. To enhance possible multi-functionality, a variety of receptor groups is desirable and is made possible through surface patterning methods. Our research focuses on the use of electrochemical techniques to uniquely design and characterize a surface coated with organic-receptor materials in a patterned geometry. We will combine our experience with surface patterning of bulk surfaces to decorate gold nanoparticles in a similar manner. Coupling the device to a spectroscopic method of Surface Plasmon Resonance Spectroscopy (SPR) will enhance our detection schemes beyond typical SPR investigations.

生物传感器是一类用于在给定环境中识别目标生物分子的设备。它们已在医学界用于疾病的早期发现和治疗，但作为发现生物战剂的方法也是可取的。因此，传感器的属性通常包括小型化尺寸、高特异性、多功能和无误报。随着传感器需求的不断变化，制造和表征传感器材料的新方法正在进行中。通常，生物识别事件是基于与底物的分子结合，存在表面修饰策略以使材料具有特定受体基团的功能化。为了增强可能的多功能性，需要多种受体群，并通过表面图案化方法使其成为可能。我们的研究重点是利用电化学技术来独特地设计和表征涂有有机受体材料的几何图形表面。我们将把我们的经验与体表面图案结合起来，以类似的方式装饰金纳米颗粒。将该装置与表面等离子体共振光谱（SPR）的光谱方法相结合，将使我们的检测方案超越典型的SPR研究。