High speed centrifuge

高速离心机

• 批准号: 345781-2007
• 负责人: Guillemette, JosephGuy
• 金额: $ 4.51万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326056
• 关键词: speed; centrifuge

A high speed centrifuge is an essential piece of equipment for researchers in biochemistry.  This instrument is used to harvest cells, sub cellular fractionation and for several of the steps involved in the purification of proteins, enzymes and nucleic acids.  It would be impossible to perform most of our experiments without this apparatus since the main focus of our work is on the fundamental properties of proteins, enzymes and nucleic acids.  We presently have two working centrifuges and a crippled 25 year old centrifuge that are shared by seven different research groups and serve over 60 scientists.  The available centrifuges include a 2 year old Beckman Avanti JE, 12 years old a Beckman Avanti J25I centrifuge and a Beckman J2-21 centrifuge that is over 25 years old.  The two Avanti centrifuges are the only ones capable of performing the tasks needed by our researchers.  We have maintained the older Beckman centrifuge due to the high demand for this type of equipment in our department.  Unfortunately the old Beckman J2-21 is no longer capable of exceeding 6,000 RPM and has recently needed expensive repairs.  Since these centrifuges usually perform for up to twenty years, our request for funding should provide seven research groups with an essential piece of shared equipment for several years to come.  Without this new centrifuge, considerable delays in the research programs of these researchers will result in significant and costly delays in their investigations.

高速离心机是生物化学研究人员必不可少的设备。该仪器用于收获细胞，亚细胞分离以及蛋白质，酶和核酸纯化中的几个步骤。如果没有该仪器，我们就不可能进行大部分实验，因为我们的工作主要集中在蛋白质的基本性质上，酶和核酸。我们目前有两台工作离心机和一台25年前报废的离心机，由七个不同的研究小组共享，为60多名科学家服务。可用的离心机包括一台2年前的贝克曼Avanti JE，12岁的贝克曼Avanti J25 I离心机和贝克曼J2- 21台离心机已经使用了25年以上。这两台Avanti离心机是唯一能够执行我们研究人员所需任务的离心机。我们保留了较旧的离心机。贝克曼离心机由于在我们部门对这种类型的设备需求量很大。不幸的是，旧的贝克曼J2-21不再能够超过6,000 RPM，最近需要昂贵的维修。由于这些离心机通常可以使用长达20年，我们申请的资金应该可以为七个研究小组提供一个在未来几年内共享的重要设备。如果没有这个新的离心机，这些研究人员的研究计划的相当大的延误将导致他们的调查的重大和昂贵的延误。