High speed, high volume centrifuge

高速大容量离心机

• 批准号: 359339-2008
• 负责人: Plettner, Erika
• 金额: $ 4.91万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=366435
• 关键词: speed; volume; centrifuge

We are four researchers in the Department of Chemistry and one researcher in the Department of Molecular Biology and Biochemistry at Simon Fraser University, and we are applying for a versatile high capacity and high speed floor-model centrifuge.  Our research is focused on the study of protein structure and function and on the discovery of new materials.  Proteins are major functional players in living organisms, and the relationship between their structure and their function is complex.  One project addresses odorant-binding proteins from insect chemosensory systems, with the aim of developing new compounds that interfere with pest insects.  A second set of projects addresses cytochrome P450, an enzyme that catalyzes one of the most difficult chemical reactions known: the insertion of an oxygen atom into a carbon-hydrogen bond.  This enzyme may be developed into catalysts in environmentally benign processes.  A third project addresses cryptochromes, proteins that are involved in the regulation of circadian rhythms.  A fourth project aims to understand the rapid molecular motions in several proteins.  This knowledge is important for the design of new drugs, pest control agents and materials.  A fifth project focuses on metal-transporting proteins, which can be developed into targeted anticancer agents. A sixth set of projects addresses bacterial pili, filamentous protein structures that are important in bacterial infection.  A seventh project specializes in the design of new nanostructured materials, for myriad applications, ranging from non-linear optic materials to imaging agents for diseased tissue.  A centrifuge is essential to all of these projects, because they require production of proteins or nanostructured materials in large quantities.  The centrifuge is used at various stages of isolation and purification of the required products. Currently we do not have sufficient access to a floor-model centrifuge for our research needs.  The requested centrifuge will enable us to produce larger quantities of the products we research more quickly.  This, in turn, will ensure a higher quality and productivity of our respective research programs.

我们是西蒙弗雷泽大学化学系的四名研究人员和分子生物与生物化学系的一名研究人员，我们正在申请一台多功能大容量高速地板式离心机。我们的研究重点是蛋白质结构和功能的研究以及新材料的发现。蛋白质是生物体的主要功能参与者，其结构与功能之间的关系是复杂的。一个项目研究昆虫化学感觉系统中的气味结合蛋白，目的是开发新的化合物来干扰害虫。第二组项目研究细胞色素P450，这是一种酶，可以催化已知最困难的化学反应之一：将氧原子插入碳氢键。该酶可开发成环境友好型工艺的催化剂。第三个项目涉及隐色素，一种参与昼夜节律调节的蛋白质。第四个项目旨在了解几种蛋白质的快速分子运动。这些知识对于设计新药、害虫防治剂和材料是很重要的。第五个项目侧重于金属运输蛋白，它可以被开发成靶向抗癌药物。第六组项目解决细菌菌毛，丝状蛋白结构，是重要的细菌感染。第七个项目专门设计新的纳米结构材料，用于各种应用，从非线性光学材料到病变组织的显像剂。离心机对所有这些项目都是必不可少的，因为它们需要大量生产蛋白质或纳米结构材料。离心机用于所需产品的各个阶段的分离和纯化。目前，我们没有足够的渠道获得地板式离心机来满足我们的研究需要。所要求的离心机将使我们能够更快地生产更多我们研究的产品。反过来，这将确保我们各自研究项目的更高质量和生产力。