Thermal cycler and laminar flow workstations for gene discovery in non-model organisms

用于非模型生物基因发现的热循环仪和层流工作站

• 批准号: 345366-2007
• 负责人: Marshall, Heather
• 金额: $ 1.97万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326082
• 关键词: Thermal; cycler; laminar; flow; workstations

The equipment in this proposal is required to support gene discovery research that aims to elucidate the genetic basic of inherited diseases in non-model organisms, particularly those of conservation, economic and management importance regionally. One immediate goal of this research is to discover the gene(s) and mutation(s) associated with hereditary hyperplastic gingivitis in silver foxes using genome-wide linkage analysis in combination with a candidate gene approach that incorporates bioinformatics tools and Real Time PCR and microarray expression analyses. The fox condition serves as a model of a similar disease in humans. Furthermore, the investigation of genes involved in these types of diseases contribute to the understanding of cellular growth and differentiation pathways and the prevalence and distribution of functionally relevant genomic variation. The requested equipment includes an Eppendorf Mastercycler Silver Block Gradient Thermal Cycler required for the amplification of the 400 microsatellite loci necessary to conduct genome-wide linkage analysis and the amplification and sequencing of candidate genes. Additionally two Envirico Tabletop Laminar Flow Workstations with Accessory UV Lights are required for the contamination-free preparation of PCR master mix cocktails and the separate addition of individual target DNAs. The additional UV light components ensure the DNA-free sterility of all reagents, pipettors, and other equipment. I am currently in the process of establishing a laboratory dedicated to gene discovery research and functional genomics of non-model, economically-important wildlife organisms. The requested equipment items are the foundation for this facility. They are vital to the attainment of my research goals and essential to any genomics-oriented laboratory. They will also serve an important role in the education and training of students.

本提案中的设备需要用于支持基因发现研究，目的是阐明非模式生物中遗传疾病的遗传基础，特别是那些在区域内具有保护、经济和管理重要性的遗传疾病。本研究的一个直接目标是发现基因（S）和突变（S）与遗传性增生性牙龈炎在银狐使用全基因组连锁分析结合候选基因的方法，结合生物信息学工具和真实的时间PCR和微阵列表达分析。狐狸的情况可以作为人类类似疾病的模型。此外，对这些类型疾病中涉及的基因的研究有助于理解细胞生长和分化途径以及功能相关基因组变异的流行和分布。申请的设备包括一台Eppendorf Mastercycler银块梯度热循环仪，用于扩增进行全基因组连锁分析所需的400个微卫星位点，以及扩增和测序候选基因。此外，还需要两个配有辅助紫外灯的Envirico桌面层流工作站，用于无污染地制备PCR主混合物混合物和单独添加单个靶DNA。额外的紫外光组件可确保所有试剂、移液器和其他设备的无DNA无菌性。我目前正在建立一个实验室，致力于基因发现研究和非模式的功能基因组学，经济上重要的野生生物。所要求的设备项目是该设施的基础。它们对实现我的研究目标至关重要，对任何以基因组学为导向的实验室都至关重要。它们还将在学生的教育和培训方面发挥重要作用。