A thermal cycler for use in plant ecological research

用于植物生态研究的热循环仪

• 批准号: 422196-2012
• 负责人: Cahill, James
• 金额: $ 0.66万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495743
• 关键词: thermal; cycler; use; plant; ecological

I am requesting funds to purchase thermal cycler to support research in plant ecology. My lab focuses on belowground processes involving plants, including competition, mycorrhizal networks, and root foraging. Because of the cryptic nature of soil organisms - including both plant roots and mycorrhizal fungi, molecular techniques are critical to species identification. My lab has developed a number of protocols for plant root species determination. We use these, along with other protocols, to answer questions related to the role of mycorrhizae in recovery following pine beetle outbreak, community assembly and phylogenetic structure, impacts of genetic diversity on ecological interactions, and niche differentiation and habitat separation. At the base of all of these studies are molecular techniques that include the amplification of DNA. For this, a high quality thermal cycler is needed. Without this equipment, my research program will take a significant hit, reducing both the quantity and quality of work my lab is able to complete.

我申请资金购买热循环仪，以支持植物生态学的研究。 我的实验室专注于涉及植物的地下过程，包括竞争，菌根网络和根觅食。 由于土壤生物（包括植物根系和菌根真菌）的隐蔽性，分子技术对物种鉴定至关重要。 我的实验室已经开发了许多用于植物根种类测定的协议。 我们使用这些，沿着与其他协议，回答有关的问题，真菌在恢复后松甲虫爆发，社区组装和系统发育结构，遗传多样性对生态相互作用的影响，生态位分化和栖息地分离的作用。 所有这些研究的基础是包括DNA扩增的分子技术。 为此，需要高质量的热循环仪。 如果没有这些设备，我的研究计划将受到重大打击，降低我的实验室能够完成的工作的数量和质量。