Dynamics of synaptic vesicle endo- and exocytosis monitored by pH-sensitive fluorescent dye

pH 敏感荧光染料监测突触小泡内吞和胞吐作用的动态

• 批准号: 238834-2006
• 负责人: Toth, Katalin
• 金额: $ 1.82万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=361025
• 关键词: Dynamics; synaptic; vesicle; endo; exocytosis

Neurons communicate with each others via chemical synapses. Synaptic events can be electrically recorded; however the interpretation of these events can be a challenging task due to the complexity of the network. Moreover, detailed information about the kinetics of synaptic vesicle pool recycling and depletion can only be obtained with techniques visualizing active synaptic vesicles. Recent developments in optical recording techniques, in combination with advances in fluorescent molecular probes provide an alternative method to study synaptic function. In the present grant we aim to investigate the secretion of synaptic vesicles at the mossy fibre system in the hippocampus. The objective of our proposal is to determine how different synaptic vesicle pools communicate with each others, how their function is modulated by different cations and, how these presynaptic functions relate to the unique physiological features of the mossy fibres. These studies will help us to better understand how neurons communicate with each other and how information is processed in the brain.

神经元之间通过化学突触进行交流。突触事件可以被电子记录；然而，由于网络的复杂性，对这些事件的解释可能是一项具有挑战性的任务。此外，有关突触囊泡池循环和耗竭的详细信息只能通过显示活跃的突触小泡的技术来获得。光学记录技术的最新发展，结合荧光分子探针的发展，为研究突触功能提供了一种新的方法。在这项研究中，我们的目标是研究海马区苔藓纤维系统中突触小泡的分泌。我们建议的目的是确定不同的突触囊泡池如何相互通信，它们的功能是如何被不同的阳离子调制的，以及这些突触前功能如何与苔藓纤维的独特生理特征相关。这些研究将帮助我们更好地了解神经元之间是如何相互通信的，以及信息是如何在大脑中处理的。