Cell line bioassay for measuring FSH bioactivity in pregnant mare serum

用于测量孕马血清中 FSH 生物活性的细胞系生物测定

• 批准号: 334972-2005
• 负责人: Price, Christopher
• 金额: $ 2.29万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Agriculture and Agri-Food Canada Research Partnership
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=361599
• 关键词: Cell; line; bioassay; measuring; FSH

Cattle breeders need reliable methods to increase production of genetically valuable animals, and to improvehereditary performance traits by selection. The common approach is inducing multiple ovulation in 'donor'cows followed by transfer of the resulting embryos into the wombs of recipient cows. Inducing multipleovulation is expensive and time-consuming, and the results can be highly variable; some cows do not respond to treatment.The cheapest and least labour-intensive drug for inducing multiple ovulation is equine chorionic gonadotropin (eCG). This is a hormone that is produced by the horse placenta during pregnancy, and is purified from horse serum. Some mares produce eCG which has a higher biological activity than that produced by other mares. However, on the horse farm this variability between mares is not taken into account when eCG is collected for use as a drug for inducing multiple ovulation in cattle. This contributes to the variation in activity of batches of commercially available eCG. Further, a large number of horsesare kept for eCG production; this number can be reduced if only the best producing mares are used.To overcome these problems, a rapid method of detecting highly active eCG is required for use at the site of eCG collection. This project aims to develop such a method. We will use an ovarian cell line, which is aculture of cells that divide continually and do not die. Using genetic engineering, we can incorporate into these cells a gene that is turned on when the cells are stimulated by eCG. The higher the activity of the eCG, the greater the expression of this gene will be. Once the gene is turned on, an enzyme is produced that will cause a chemical in the incubation medium to change colour. Thus the activity of eCG can be readily detected by comparing the colour of the cells to a standardized colour chart. This will enable the Canadian pharmaceutical industry to produce a better product, and to reduce the number of horses required to make eCG. This will result in significant economic savings for Canadian cattle breeders

养牛者需要可靠的方法来增加具有遗传价值的动物的产量，并通过选择来改善遗传性能特征。常见的方法是诱导“供体”奶牛多次排卵，然后将所得胚胎移植到受体奶牛的子宫中。诱导多次排卵既昂贵又耗时，而且结果变化很大；一些奶牛对治疗没有反应。诱导多次排卵最便宜且劳动强度最小的药物是马绒毛膜促性腺激素 (ECG)。这是一种由马胎盘在怀孕期间产生的激素，是从马血清中纯化出来的。有些母马产生的心电图比其他母马产生的生物活性更高。然而，在马场，当收集心电图用作诱导牛多次排卵的药物时，不会考虑母马之间的这种差异。这导致了市售心电图批次的活性变化。此外，还饲养了大量的马匹用于心电图制作；如果只使用产力最好的母马，这个数字就可以减少。为了克服这些问题，需要在心电图采集现场使用一种快速检测高活性心电图的方法。该项目旨在开发这样一种方法。我们将使用卵巢细胞系，它是不断分裂且不会死亡的细胞培养物。利用基因工程，我们可以将一个基因整合到这些细胞中，当细胞受到心电图刺激时，该基因就会被打开。 eCG的活性越高，该基因的表达量就越大。一旦基因被打开，就会产生一种酶，导致培养介质中的化学物质改变颜色。因此，通过将细胞的颜色与标准化比色卡进行比较，可以轻松检测心电图的活动。这将使加拿大制药业能够生产出更好的产品，并减少制作心电图所需的马匹数量。这将为加拿大养牛者节省大量经济开支