Digitized point-of-care diagnostics: Reading disc-based bioassays with a standard computer drive

数字化现场护理诊断：使用标准计算机驱动器读取基于光盘的生物测定结果

• 批准号: 356591-2007
• 负责人: Yu, HuaZhong
• 金额: $ 4.32万
• 依托单位: Simon Fraser University
• 依托单位国家: 加拿大
• 项目类别: Idea to Innovation
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=363210
• 关键词: Digitized; point; care; diagnostics; Reading

Microarray technology is a powerful tool for high-throughput analysis of biomolecular interactions, which is important for gene profiling, immunoassays, and drug discovery. However, its application is currently restrictec to biomedical laboratories or hospital settings with extensive infrastructure (e.g., robotic spotter, fluorescence scanner). Compact disc (CD)-based biomolecular screening is a promising, inexpensive alternative, especially suitable for point-of-care clinical diagnostics (e.g., in a doctor's office or remote areas). The direct adaptation of standard desktop/laptop computers and accessories for rapid biomedical diagnosis would constitute a major breakthrough in the field of health research: the optical drive can serve as detector and the plastic CD/DVD base as platform for the preparation of binding assays.This joint "Idea-to-innovation (121) Phase 1" project highlights the application potential of digitized biomolecular screening and provides a unique collaborative opportunity for a group of chemists, molecular biologists, and electrical engineers. The goals of the proposed research are the construction of DNA/protein microarrays on CD, validation with conventional fluorescence scanning, testing of standard bioassays (biotin-streptavidin) with unmodified computer drives, and digital reading of DNA hybridization microarrays (both complementary and mismatched target strands), followed by extension to biomedical samples (PCR products) and to protein assays. The success of this project may lead to the commercialization of an inexpensive, portable diagnostic system for near-patient clinical testing and on-site monitoring.

微阵列技术是生物分子相互作用高通量分析的有力工具，在基因分析、免疫分析和药物发现方面具有重要意义。然而，它的应用目前仅限于生物医学实验室或具有广泛基础设施的医院环境（例如，机器人观察者，荧光扫描仪）。基于光盘（CD）的生物分子筛选是一种有前途的、廉价的替代方法，特别适用于即时护理的临床诊断（例如，在医生办公室或偏远地区）。将标准台式/笔记本电脑及其附件直接用于快速生物医学诊断，将构成健康研究领域的重大突破：光驱可作为检测器，塑料CD/DVD底座可作为准备结合分析的平台。这个联合的“从想法到创新（121）第一阶段”项目突出了数字化生物分子筛选的应用潜力，并为化学家、分子生物学家和电气工程师提供了一个独特的合作机会。拟议研究的目标是在CD上构建DNA/蛋白质微阵列，使用常规荧光扫描进行验证，使用未修改的计算机驱动器测试标准生物测定（生物素-链亲和素），以及DNA杂交微阵列（互补和不匹配的目标链）的数字读取，然后扩展到生物医学样品（PCR产物）和蛋白质测定。该项目的成功可能会导致一种廉价的便携式诊断系统的商业化，用于近患者临床测试和现场监测。