Origins of variation in muscle energetics

肌肉能量变化的起源

• 批准号: 170572-2006
• 负责人: Moyes, Christopher
• 金额: $ 3.54万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=363372
• 关键词: Origins; variation; muscle; energetics

My program explores the origins and consequences of variation in muscle energy production: how muscle metabolism is remodelled when needed and how differences in metabolic machinery arise between tissues and animals as a consequence of evolution and development. In the next grant cycle we explore how the genes in muscle are regulated to control the levels of bioenergetic enzymes.     1. PGC1 is a gene regulator that appears to be important in determining the mitochondrial content of muscle in many contexts. We will examine how PGC1 and related genes arose early in the evolutionary history of vertebrates. We will also study how structural peculiarities in PGC1 of primitive vertebrates affect its function.     2. Each muscle possesses properties that are tailored to different types of activity. For example, the enzymes of energy metabolism occur at levels that can support either longterm exercise or short duration, burst exercise. Since each muscle has the same complement of genes, it is not clear how these differences arise between muscles. We will study how muscles become specialized by comparing the levels of gene regulators in nuclei of different muscles.     3. Animals with an ability to tolerate low levels of oxygen have many physiological and biochemical specializations. Muscles, for example, differ in the levels of enzymes that can produce energy without the need for oxygen. We will examine the factors that regulate the genes for these enzymes in three related fish that differ in oxygen dependency.    By comparing and contrasting models and experimental approaches, we hope to distinguish between regulatory pathways that are broadly conserved among animals and those that are responsible for unique features of specific tissues and animals.

我的课程探讨了肌肉能量产生变化的起源和后果：肌肉代谢如何在需要时被重塑，以及作为进化和发展的结果，组织和动物之间代谢机制的差异是如何产生的。在下一个资助周期中，我们将探索如何调节肌肉中的基因来控制生物能酶的水平。1. PGC1是一种基因调节因子，在许多情况下似乎在决定肌肉线粒体含量方面很重要。我们将研究PGC1和相关基因是如何在脊椎动物的进化史中出现的。我们还将研究原始脊椎动物PGC1的结构特性如何影响其功能。2. 每块肌肉都有适合不同类型活动的特性。例如，能量代谢酶的水平既可以支持长期运动，也可以支持短时间的爆发性运动。由于每块肌肉都有相同的基因补体，所以还不清楚这些差异是如何在肌肉之间产生的。我们将通过比较不同肌肉细胞核中基因调节因子的水平来研究肌肉是如何变得专门化的。3. 有能力忍受低氧水平的动物有许多生理和生化特征。例如，肌肉在不需要氧气就能产生能量的酶的水平上有所不同。我们将研究在三种不同氧依赖性的相关鱼类中调节这些酶基因的因素。通过比较和对比模型和实验方法，我们希望区分在动物中广泛保守的调控途径和那些负责特定组织和动物的独特特征的调控途径。