HPLC for nucleoside and metabolite measurement

用于核苷和代谢物测量的 HPLC

• 批准号: 360078-2008
• 负责人: Blay, Jonathan
• 金额: $ 5.68万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=366441
• 关键词: HPLC; nucleoside; metabolite; measurement

We are investigating how the local tissue environment affects the behaviour of individual cells - their ability to divide, to relocate within the tissue, or to fulfil the unique functions inherent to their cell type. One factor that dramatically changes cell behaviour in this context is deprivation of oxygen ('hypoxia') and nutrients. In hypoxic tissues the energy metabolism of the cells is changed, and there is a dramatic release of certain chemicals that are involved in the energy supply cycle. One of the most important is adenosine, which our research has shown is able to alter cell proliferation, enhance the ability of cells to move, and modify the range of regulatory proteins that the cell has on its outer surface. This unassuming, simple regulator has probably played an important part in coordinating cell behaviour throughout much of evolution, and is important in human prenatal development, defence mechanisms and disease.   We showed some time ago that chronically hypoxic tissue has levels of adenosine that are about 100-fold higher than in normal tissues. However, our efforts since then, to measure the changes in adenosine in certain situations and to relate them to the changes we see, have been hampered by the lack of a reliable means of measuring this chemical. The equipment that we have received through this program will allow us to make a major leap forward, and to perform careful measurements not only of adenosine but also of other important metabolites. Not only will this help us in our understanding of a very fundamental process, but it will also enable us to move our work toward exploiting this phenomenon for scientific and therapeutic benefit.

我们正在研究局部组织环境如何影响单个细胞的行为--它们分裂的能力，在组织内重新定位的能力，或履行其细胞类型固有的独特功能的能力。在这种情况下，一个显著改变细胞行为的因素是缺乏氧气(‘低氧’)和营养。在低氧组织中，细胞的能量新陈代谢发生变化，能量供应周期中涉及的某些化学物质急剧释放。其中最重要的是腺苷，我们的研究表明，它能够改变细胞的增殖，增强细胞的移动能力，并改变细胞外表面的调节蛋白范围。这种不张扬的、简单的调节器可能在整个进化过程中协调细胞行为方面发挥了重要作用，在人类产前发育、防御机制和疾病中也很重要。我们不久前表明，慢性缺氧组织中的腺苷水平大约是正常组织的100倍。然而，自那时以来，我们一直在努力测量某些情况下腺苷的变化，并将它们与我们所看到的变化联系起来，但由于缺乏测量这种化学物质的可靠手段，我们的努力一直受到阻碍。我们通过这个项目获得的设备将使我们能够实现重大飞跃，不仅可以对腺苷进行仔细的测量，还可以对其他重要的代谢物进行仔细的测量。这不仅有助于我们理解一个非常基本的过程，还将使我们能够推动我们的工作，使我们能够利用这种现象来获得科学和治疗方面的好处。